Protein Recovery Using Fibrous Materials

Chen, Li Ang; Serad, George A.; Carbonell, Ruben G.

doi:10.1007/978-1-4757-2521-6_6

Cited by 4 publications

(8 citation statements)

References 14 publications

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“…Microscopic observations showed that salted out WSCA consists of spherical particles of around 1 mm that are agglomerated into bigger¯ocs. 24 This is different from the micro®brillar structure of CAF or CTF, with dimensions on the order of tens and hundreds of microns. Figure 5 shows that the protein removal by ®ltering the BSA precipitates through the 20 mm frit or through a CAF layer 20 mm frit resulted in similarly low recoveries.…”

Section: Effect Of the Acetyl Contentmentioning

confidence: 97%

“…12 Figure 4 compares the performance of salt-induced BSA recovery in the presence of WSCA, CAF and CTF, which have increasing DS: 0.6±0.8 for WSCA, 2.4 for CAF and 3.0 for CTF. As the acetyl content of the cellulose acetate increased, less salt was required to remove the same amount of protein.…”

Section: Effect Of the Acetyl Contentmentioning

confidence: 99%

“…12 Charged cotton ®bers resulted in good protein recovery but required high dosages (100 g dyed cotton per g BSA). Cellulose triacetate ®brets (CTF) resulted in high BSA recovery at moderate dosages (30 g CTF per g BSA) and moderate salt concentration (15% (w/v) ammonium sulfate saturation).…”

Section: Introductionmentioning

confidence: 99%

“…12 Cellulose acetate is produced from waste cotton ®bers, 14 or by direct acetylation of pulp and mill sludge. 15 Depending on the degree of substitution (DS), ie the number of acetyl groups contained in a unit glucose ring, various types of cellulose acetate can be obtained, and some of these were studied in the present work: water-soluble cellulose acetate (WSCA) at a DS of 0.6±0.8, CAF at a DS of 2.4 and CTF at a DS of 3.0.…”

Section: Introductionmentioning

confidence: 99%

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Recovery of proteins and other biological compounds using fibrous materials: I. Adsorption by salt addition

Chen

Carbonell

Serad

1999

J. Chem. Technol. Biotechnol.

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An adsorption and ®ltration process for the recovery of proteins and other biological compounds from aqueous streams has been developed, using cellulose-based ®brous materials. Of the many cellulose derivatives studied, cellulose acetate ®brets (CAF) and cellulose triacetate ®brets (CTF) have been shown to be the most effective. In the presence of salts, they lead to protein adsorption by hydrophobic interactions. Model proteins, such as bovine serum albumin (BSA), have been recovered by incubating these solutions with CTF in the presence of ammonium sulfate, followed by ®ltration through a 20 mm pore size ®lter. The amount of salt necessary varies with the protein type, but decreases with increasing temperature and protein concentration. High protein recovery has been obtained from an actual wastewater system at low salt dosages.

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Section: Effect Of the Acetyl Contentmentioning

confidence: 97%

Section: Effect Of the Acetyl Contentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Recovery of proteins and other biological compounds using fibrous materials: I. Adsorption by salt addition

Chen

Carbonell

Serad

1999

J. Chem. Technol. Biotechnol.

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“…It is often termed as a polymeric-electrolyte with dual characteristics of a polymer and an electrolyte. The most significant property of polyelectrolytes turns out to be the water solubility, which opens the door for various applications in food industry, 1, 2 ultrafiltration, 3, 4 waste water treatment, 5, 6 fuel cells, 7–10 biomaterials 11–14 and nanotechnology. 15–18 Cationic polyelectrolytes have basic groups such as phosphonium, sulfonium, ammonium, imidazolium or pyridinium in their structure, while anionic polyelectrolytes have acidic groups like sulfonate, phosphate, carboxylate, phosphonate and arsenate.…”

Section: Introductionmentioning

confidence: 99%

Binding of Cobaltocenium-Containing Polyelectrolytes with Anionic Probes

Pageni

Kabir

Yang

et al. 2017

J Inorg Organomet Polym

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Cationic cobaltocenium-containing polyelectrolytes have a unique ability to form ionic complex with various anionic species. We carried out two sets of model study to compare the relative binding strength of a cobaltocenium-containing polyelectrolyte. First, the nature and relative strength of intermolecular interaction between cobaltocenium-containing polyelectrolytes and different anionic probes were investigated by spectroscopic methods. A dye-displacement method was used to monitor absorbance and fluorescence emissions. Second, the binding strength of this cobaltocenium-containing polyelectrolyte was compared with a classical quaternary ammonium polymer. Formation of polyelectrolyte complex between the cobaltocenium-containing polyelectrolyte and a common anionic polyelectrolyte at various concentrations was examined by optical absorption and light scattering.

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Recovery of proteins and other biological compounds using fibrous materials: II. Flocculation by polyelectrolyte addition

Chen

Carbonell

Serad

1999

J. Chem. Technol. Biotechnol.

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Polyelectrolytes have been used in wastewater treatment processes to destabilize colloidal suspensions of proteins, cells and other biological compounds, resulting in¯occulation. When a solution containing a single model protein, bovine serum albumin (BSA), is treated with a polyelectrolyte, carboxymethyl cellulose (CMC), large and strong¯ocs are formed, which are easily retained by a 20 mm pore size ®lter. However, when a mixture of proteins, cells, and fats from an actual wastewater sample is treated in the same manner, smaller and weaker¯ocs are observed. An adsorption and ®ltration process for the recovery of valuable biological compounds using cellulosebased ®brous materials has been developed. When used simultaneously with CMC, cellulose acetate and triacetate ®brets (CAF and CTF) resulted in high recovery of biomolecules from solution at very low dosages of both polyelectrolyte and ®brets. CMC interacts with biomolecules by electrostatic interactions and polymer bridging, while CTF/CAF facilitate¯oc growth by adsorption and bridging of primary particles and by entrapment of small aggregates within their highly ®brillated microstructure.

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Protein Recovery Using Fibrous Materials

Cited by 4 publications

References 14 publications

Recovery of proteins and other biological compounds using fibrous materials: I. Adsorption by salt addition

Recovery of proteins and other biological compounds using fibrous materials: I. Adsorption by salt addition

Binding of Cobaltocenium-Containing Polyelectrolytes with Anionic Probes

Recovery of proteins and other biological compounds using fibrous materials: II. Flocculation by polyelectrolyte addition

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