Protein purification by affinity precipitation

Functional Glyco-Affinity Precipitation/Capturing for Enhanced Affinity Proteomics

“…The glyco-affinity precipitations described above are the so-called primary effect affinity precipitation (Hilbrig et al, 2003). The precipitation is the direct result of the formation of affinity complex through multivalent binding interactions between multivalent carbohydrates and multivalent binding sites in the proteins.…”

“…This stimulusresponsive affinity precipitation is so-called secondary effect affinity precipitation or indirect precipitation and has been well reviewed recently (see review Freitag, 1998;Hilbrig et al, 2003). The basic stimulus-responsive affinity precipitation is outlined with a thermal-responsive glyco-affinity precipitation in Fig.…”

“…In particular, functional affinity precipitation, i.e. simple environmental change, such as temperature and pH triggered affinity precipitation, has been the focus of growing attention as an effective purification approach through selective recovery of target proteins (Hilbrig et al, 2003). Notably, in the functional affinity precipitation process, both capture and release of target protein are conducted in solution and separation of target protein from non-target ones is performed between pellet and supernatant, both which highly improve the efficiency of the purification.…”

Development of an ELP-Z based mAb affinity precipitation process using scaled-down filtration techniques

“…However, relatively high resin costs and slow volumetric throughputs for this step are often an impediment for dealing with increasingly higher upstream mAb titers. Affinity precipitation, on the other hand, may offer advantages as a mAb capture step in terms of scalability and processing simplicity at reduced costs (Hilbrig and Freitag, 2003).…”

Adsorption of human IgG on to poly(N-isopropylacrylamide)-based polymer particles

“…PNIPAM macroligands are particularly useful for the design and synthesis of heterobifunctional conjugates for affinity precipitation (Fong et al 2002). Affinity precipitation is a highly promising alternative technique for affinity chromatography in downstream processing for high value proteins, namely for the purification of antibodies (Taipa et al 2000;Kumar et al 2003;Kim et al 2005) as it combines the simplicity and easy performance of precipitation methods with the specificity and selectivity of affinity interactions (Hilbrig and Freitag 2003;Roque et al 2004). Nevertheless, the commercial application of this methodology has been limited by the availability of specific macroligands with minimal non-specific adsorption.…”