Protein-protein interactions in the yeastPKC1 pathway: Pkc1p interacts with a component of the MAP kinase cascade

Paravicini, Gerhard; Friedli, Laurence

doi:10.1007/bf02174117

Cited by 20 publications

(19 citation statements)

References 57 publications

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“…Interestingly, however, two-hybrid analysis indicates that Pkc1p interacts with Mkk1p but not with the immediate downstream kinase, Bck1p. Mkk1p also interacts with Bck1p and Mkk2p, and Mkk2p interacts with Slt2/Mpk1p (450,567). Taken together, these observations indicate that, as in the HOG pathway, the MAPKK could play an important role in mediating the formation of a complex between pathway components.…”

Section: Cell Integrity Pathwaymentioning

confidence: 79%

Osmotic Stress Signaling and Osmoadaptation in Yeasts

Hohmann

2002

Microbiol Mol Biol Rev

1,486

1,431

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The ability to adapt to altered availability of free water is a fundamental property of living cells. The principles underlying osmoadaptation are well conserved. The yeast Saccharomyces cerevisiae is an excellent model system with which to study the molecular biology and physiology of osmoadaptation. Upon a shift to high osmolarity, yeast cells rapidly stimulate a mitogen-activated protein (MAP) kinase cascade, the high-osmolarity glycerol (HOG) pathway, which orchestrates part of the transcriptional response. The dynamic operation of the HOG pathway has been well studied, and similar osmosensing pathways exist in other eukaryotes. Protein kinase A, which seems to mediate a response to diverse stress conditions, is also involved in the transcriptional response program. Expression changes after a shift to high osmolarity aim at adjusting metabolism and the production of cellular protectants. Accumulation of the osmolyte glycerol, which is also controlled by altering transmembrane glycerol transport, is of central importance. Upon a shift from high to low osmolarity, yeast cells stimulate a different MAP kinase cascade, the cell integrity pathway. The transcriptional program upon hypo-osmotic shock seems to aim at adjusting cell surface properties. Rapid export of glycerol is an important event in adaptation to low osmolarity. Osmoadaptation, adjustment of cell surface properties, and the control of cell morphogenesis, growth, and proliferation are highly coordinated processes. The Skn7p response regulator may be involved in coordinating these events. An integrated understanding of osmoadaptation requires not only knowledge of the function of many uncharacterized genes but also further insight into the time line of events, their interdependence, their dynamics, and their spatial organization as well as the importance of subtle effects

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Section: Cell Integrity Pathwaymentioning

confidence: 79%

Osmotic Stress Signaling and Osmoadaptation in Yeasts

Hohmann

2002

Microbiol Mol Biol Rev

1,486

1,431

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“…Mutation of Thr1119 to Ala, Cys, or Tyr also results in constitutive signaling (D. E. Levin and A. K. Sobering, unpublished), suggesting that disruption of an interaction involving Thr1119 (either by phosphorylation or mutation) is the key to activation of this MEKK. Bck1 is presumed to phosphorylate and activate Mkk1/2 based on genetic epistasis studies, two-hybrid interaction, and its requirement for activation of Mpk1 (137,148,254). Mkk1/2 phosphorylate Mpk1 on neighboring tyrosyl and threonyl residues in a T-X-Y motif that is diagnostic for MAP kinases.…”

Section: Cwi Map Kinase Cascadementioning

confidence: 99%

Cell Wall Integrity Signaling inSaccharomyces cerevisiae

Levin¹

2005

Microbiol Mol Biol Rev

1,036

1,208

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The yeast cell wall is a highly dynamic structure that is responsible for protecting the cell from rapid changes in external osmotic potential. The wall is also critical for cell expansion during growth and morphogenesis. This review discusses recent advances in understanding the various signal transduction pathways that allow cells to monitor the state of the cell wall and respond to environmental challenges to this structure. The cell wall integrity signaling pathway controlled by the small G-protein Rho1 is principally responsible for orchestrating changes to the cell wall periodically through the cell cycle and in response to various forms of cell wall stress. This signaling pathway acts through direct control of wall biosynthetic enzymes, transcriptional regulation of cell wall-related genes, and polarization of the actin cytoskeleton. However, additional signaling pathways interface both with the cell wall integrity signaling pathway and with the actin cytoskeleton to coordinate polarized secretion with cell wall expansion. These include Ca2+ signaling, phosphatidylinositide signaling at the plasma membrane, sphingoid base signaling through the Pkh1 and -2 protein kinases, Tor kinase signaling, and pathways controlled by the Rho3, Rho4, and Cdc42 G-proteins

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“…Pkc1 phosphorylates Bck1 in vitro at several sites in a hinge region between its putative regulatory domain and its catalytic domain Levin 2005) that is also the site of activating mutations (Lee and Levin 1992). Bck1 is presumed to phosphorylate and activate Mkk1/2 on the basis of genetic epistasis studies, two-hybrid interactions, and its requirement for activation of Mpk1 Kamada et al 1995;Paravicini and Friedli 1996;Ho et al 2002). Mkk1 and -2 phosphorylate Mpk1 on neighboring tyrosyl and threonyl residues in a T-X-Y motif within the activation loop conserved among MAPKs.…”

Section: Pkc1 and The Cwi Mapk Cascadementioning

confidence: 99%

Regulation of Cell Wall Biogenesis in Saccharomyces cerevisiae: The Cell Wall Integrity Signaling Pathway

2011

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The yeast cell wall is a strong, but elastic, structure that is essential not only for the maintenance of cell shape and integrity, but also for progression through the cell cycle. During growth and morphogenesis, and in response to environmental challenges, the cell wall is remodeled in a highly regulated and polarized manner, a process that is principally under the control of the cell wall integrity (CWI) signaling pathway. This pathway transmits wall stress signals from the cell surface to the Rho1 GTPase, which mobilizes a physiologic response through a variety of effectors. Activation of CWI signaling regulates the production of various carbohydrate polymers of the cell wall, as well as their polarized delivery to the site of cell wall remodeling. This review article centers on CWI signaling in Saccharomyces cerevisiae through the cell cycle and in response to cell wall stress. The interface of this signaling pathway with other pathways that contribute to the maintenance of cell wall integrity is also discussed.

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Protein-protein interactions in the yeastPKC1 pathway: Pkc1p interacts with a component of the MAP kinase cascade

Cited by 20 publications

References 57 publications

Osmotic Stress Signaling and Osmoadaptation in Yeasts

Osmotic Stress Signaling and Osmoadaptation in Yeasts

Cell Wall Integrity Signaling inSaccharomyces cerevisiae

Regulation of Cell Wall Biogenesis in Saccharomyces cerevisiae: The Cell Wall Integrity Signaling Pathway

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