Protein-protein interactions in plant antioxidant defense

Melicher, Pavol; Dvořák, Petr; Šamaj, Jozef; Takáč, Tomáš

doi:10.3389/fpls.2022.1035573

Cited by 11 publications

(6 citation statements)

References 271 publications

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“…Reactive oxygen species (ROS) are components of multiple metabolic and developmental pathways in plants under normal and stress conditions [43]. At low concentrations, ROS perform signaling functions and are involved in regulating plant growth and development and plant response to stress.…”

Section: Discussionmentioning

confidence: 99%

TMT-Based Proteomic Analysis Reveals the Molecular Mechanisms of Sodium Pheophorbide A against Black Spot Needle Blight Caused by Pestalotiopsis neglecta in Pinus sylvestris var. mongolica

Zhang,

Yang,

Wang

et al. 2024

JoF

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Black spot needle blight is a minor disease in Mongolian Scots pine (Pinus sylvestris var. mongolica) caused by Pestalotiopsis neglecta, but it can cause economic losses in severe cases. Sodium pheophorbide a (SPA), an intermediate product of the chlorophyll metabolism pathway, is a compound with photoactivated antifungal activity, which has been previously shown to inhibit the growth of P. neglecta. In this study, SPA significantly reduced the incidence and disease index and enhanced the chlorophyll content and antioxidant enzyme activities of P. sylvestris var. mongolica. To further study the molecular mechanism of the inhibition, we conducted a comparative proteomic analysis of P. neglecta mycelia with and without SPA treatment. The cellular proteins were obtained from P. neglecta mycelial samples and subjected to a tandem mass tag (TMT)-labelling LC-MS/MS analysis. Based on the results of de novo transcriptome assembly, 613 differentially expressed proteins (DEPs) (p < 0.05) were identified, of which 360 were upregulated and 253 downregulated. The 527 annotated DEPs were classified into 50 functional groups according to Gene Ontology and linked to 256 different pathways using the Kyoto Encyclopedia of Genes and Genomes database as a reference. A joint analysis of the transcriptome and proteomics results showed that the top three pathways were Amino acid metabolism, Carbohydrate metabolism, and Lipid metabolism. These results provide new viewpoints into the molecular mechanism of the inhibition of P. neglecta by SPA at the protein level and a theoretical basis for evaluating SPA as an antifungal agent to protect forests.

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Section: Discussionmentioning

confidence: 99%

TMT-Based Proteomic Analysis Reveals the Molecular Mechanisms of Sodium Pheophorbide A against Black Spot Needle Blight Caused by Pestalotiopsis neglecta in Pinus sylvestris var. mongolica

Zhang,

Yang,

Wang

et al. 2024

JoF

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“…Protein content is a vital component of the system that controls reactive oxygen species. The enzymes' folding, stability, degradation, and activation are controlled by proteins, and these processes have significant effects on ROS buildup and plant stress tolerance [70]. Compression has shown that total phenols (TPs) are active during the process of plant growth and play a significant positive role in the plant growth mechanism [71].…”

Section: Interpretation To Mgo Nps In Biochemical Characterizationmentioning

confidence: 99%

Influence of Magnesium Oxide (MgO) Nanoparticles on Maize (Zea mays L.)

Abbas,

Hassan,

Huang

et al. 2024

Agronomy

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An approximate revolution synthesis of magnesium oxide (MgO) nanoparticles has been prepared. For plant growth and development, MgO is essential. The effect and efficiency, respectively, in seed germination, seedling growth, and plant growth were also studied. These analyses examined maize with different concentrations and parameters. The concentration of 500 ppm was tested with extreme results in areas such as plant height, protein contents both in-vivo and in-vitro, and MgO effects shown both in shoot (12.83 ± 0.5 cm) and root (5.37 ± 0.5 cm). Maximum confirmations were fixed with the help of MgO NPs characterization through TEM, SEM, FTIR, zeta potential, and X-ray. The effect of MgO NPs showed a significant increase in root and shoot length, and simultaneous in-vivo studies also showed significant results in plant physiological parameters. In effect, the vital performance in germination rate, potential, and index MgO NPs was higher than the control. Moreover, the performance and absorption of MgO NPs was confirmed by physiological characterization with the help of a UV–Vis spectrophotometer applied to the leaves and roots. It was demonstrated that the influence of MgO NPs is positive and potentially can be used for seedling growth and also for plants. It may bolster farming methods, and help maintain high food quality, quantity, and production.

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“…(2020) found a viral protein that moved from the plasma membrane to chloroplasts upon plant sensing of biotic threats, suggesting a protein‐mediated regulatory defence pathway linking the plasma membrane and chloroplasts. Moreover, protein–protein interactions such as folding, stabilisation, degradation and activation play important roles in ROS scavenging within diverse metabolic and physiological processes, but are understudied (Melicher et al ., 2022).…”

Section: Defence In the Symplastic Spacementioning

confidence: 99%

Strategies of plants to overcome abiotic and biotic stresses

Du,

Haensch,

Alfarraj

et al. 2024

Biological Reviews

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In their environment, plants are exposed to a multitude of abiotic and biotic stresses that differ in intensity, duration and severity. As sessile organisms, they cannot escape these stresses, but instead have developed strategies to overcome them or to compensate for the consequences of stress exposure. Defence can take place at different levels and the mechanisms involved are thought to differ in efficiency across these levels. To minimise metabolic constraints and to reduce the costs of stress defence, plants prioritise first‐line defence strategies in the apoplastic space, involving ascorbate, defensins and small peptides, as well as secondary metabolites, before cellular processes are affected. In addition, a large number of different symplastic mechanisms also provide efficient stress defence, including chemical antioxidants, antioxidative enzymes, secondary metabolites, defensins and other peptides as well as proteins. At both the symplastic and the apoplastic level of stress defence and compensation, a number of specialised transporters are thought to be involved in exchange across membranes that still have not been identified, and information on the regeneration of different defence compounds remains ambiguous. In addition, strategies to overcome and compensate for stress exposure operate not only at the cellular, but also at the organ and whole‐plant levels, including stomatal regulation, and hypersensitive and systemic responses to prevent or reduce the spread of stress impacts within the plant. Defence can also take place at the ecosystem level by root exudation of signalling molecules and the emission of volatile organic compounds, either directly or indirectly into the rhizosphere and/or the aboveground atmosphere. The mechanisms by which plants control the production of these compounds and that mediate perception of stressful conditions are still not fully understood. Here we summarise plant defence strategies from the cellular to ecosystem level, discuss their advantages and disadvantages for plant growth and development, elucidate the current state of research on the transport and regeneration capacity of defence metabolites, and outline insufficiently explored questions for further investigation.

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Protein-protein interactions in plant antioxidant defense

Cited by 11 publications

References 271 publications

TMT-Based Proteomic Analysis Reveals the Molecular Mechanisms of Sodium Pheophorbide A against Black Spot Needle Blight Caused by Pestalotiopsis neglecta in Pinus sylvestris var. mongolica

TMT-Based Proteomic Analysis Reveals the Molecular Mechanisms of Sodium Pheophorbide A against Black Spot Needle Blight Caused by Pestalotiopsis neglecta in Pinus sylvestris var. mongolica

Influence of Magnesium Oxide (MgO) Nanoparticles on Maize (Zea mays L.)

Strategies of plants to overcome abiotic and biotic stresses

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