Protein profiling identified dissociations between growth hormone-mediated longitudinal growth and bone mineralization in short prepubertal children

Protein markers predict body composition during growth hormone treatment in short prepubertal children

“…Within this study, much effort was put into developing and testing existing strategies to identify the proteins represented by the peaks of interest. So far, we have not identified any more protein peaks than previously reported . The difficulty to identify the proteins corresponding to the peaks of interest is considered to be the main limitation of the method chosen in this study.…”

“…Different isoforms of these proteins have previously been associated with longitudinal bone growth and bone mineralization response to GH treatment in short prepubertal children, suggesting that these proteins may play a role in tuning GH response . Interestingly, there are discrepancies regarding different isoforms and the association with GH effects on specific body compartments.…”

“…Transthyretin and haemoglobin subunit α (HBA) were found to be associated with effects on total body FM. We have previously shown other specific isoforms of TTR to be associated with the bone mineralization and growth response to GH treatment . The expression of TTR is under the control of hepatocyte nuclear factor 6 (HNF‐6), which is activated by GH .…”

“…Sets of potential regression models were generated using between 1 and 10 peaks. Data were analysed using forward stepwise selection (StepwiseForwardP) and stepwise selection (StepwiseP) using fine‐tuning with lower P ‐values to select variables for inclusion in the model, least absolute shrinkage and selection operator (Lasso), a rescaled version of Lasso (ConeLasso) and orthogonal matching pursuit (OMP) as previously described . The threshold for inclusion within both the StepwiseForwardP and StepwiseP models was set to a probability of 5% ( P = 0·05).…”

“…The function of these newly identified factors then needs to be tested using other systems. Today, we have proteomic tools to identify not only biomarkers related to longitudinal growth, but also those associated with bone mass in short prepubertal children …”