Protein profile of the HeLa cell line

Fountoulakis, Michael; Tsangaris, George Th.; Oh, Jieun; Maris, Antony; Lubec, Gert

doi:10.1016/j.chroma.2004.03.032

Cited by 35 publications

(35 citation statements)

References 40 publications

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“…By comparison, DNA and RNA-binding proteins comprise only 10.3% of total nuclear proteome of human fibroblasts. 27 Many of the identified proteins (14, or 41.2%) play a role in transcriptional regulation, e.g. transcriptional factors, activators, and repressors (Figure 4C).…”

Section: Resultsmentioning

confidence: 99%

Mechlorethamine-Induced DNA–Protein Cross-Linking in Human Fibrosarcoma (HT1080) Cells

et al. 2011

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Antitumor nitrogen mustards, such as bis(2-chloroethyl)methylamine (mechlorethamine), are useful chemotherapeutic agents with a long history of clinical application. The antitumor effects of nitrogen mustards are attributed to their ability to induce DNA-DNA and DNA-protein cross-links (DPCs) that block DNA replication. In the present work, a mass spectrometry based methodology was employed to characterize in vivo DNA-protein cross-linking following treatment of human fibrosarcoma (HT1080) cells with cytotoxic concentrations of mechlorethamine. A combination of mass spectrometry-based proteomics and immunological detection was used to identify 38 nuclear proteins which were covalently cross-linked to chromosomal DNA following treatment with mechlorethamine. Isotope dilution HPLC-ESI+-MS/MS analysis of total proteolytic digests revealed a concentration-dependent formation of N-[2-(S-cysteinyl)ethyl]-N-[2-(guan-7-yl)ethyl]methylamine (Cys-N7G-EMA) conjugates, indicating that mechlorethamine cross-links cysteine thiols within proteins to N-7 positions of guanine in DNA.

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Section: Resultsmentioning

confidence: 99%

Mechlorethamine-Induced DNA–Protein Cross-Linking in Human Fibrosarcoma (HT1080) Cells

et al. 2011

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“…Although expression level profiles of cells, including telomerase transfected cells [28], have been extensively studied at mRNA level, this is to our knowledge the first study that compares hTERT overexpressing cells with their normal parental cells by a proteomic analysis. Since especially in eukaryotic cells mRNA and protein levels are poorly correlated [71,72], such a comparison at protein level is of high interest. In the present study, a good correlation between protein and mRNA level was observed only in the cases of cytokeratin 7, which is in accordance with a previous report showing that cell-structure-related proteins show a high correlation between mRNA and protein levels [72], and glutathione S-transferase P. The uncharacterized FBXO21 shows no different regulation at mRNA level at all.…”

Section: Discussionmentioning

confidence: 99%

Comparison of early passage, senescent and hTERT immortalized endothelial cells

Chang

Grillari‐Voglauer

Mayrhofer

et al. 2005

Experimental Cell Research

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“…We have therefore utilised a proteomic approach to begin investigating the response of in vitro cultured HeLa cells to sub-physiological temperature culturing. Although HeLa cells are not used industrially, the advantage of using these as model system is the availability of the human genome, unlike the CHO genome, and the availability of well characterised 2D-PAGE maps of HeLa cells in the public domain (Fountoulakis et al 2004). Further, we wished to investigate whether those responses observed in CHO cells to date were conserved in other mammalian cell lines.…”

Section: Introductionmentioning

confidence: 99%

The cold-shock response in mammalian cells: investigating the HeLa cell cold-shock proteome

Underhill

Smales

2007

Cytotechnology

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In recent years there have been a number of reports that suggest the sub-physiological (<37°C) temperature in vitro culturing of mammalian cells can result in enhanced heterologous protein production. Despite these reports, the mechanisms by which mammalian cells respond to such conditions are largely unknown. We therefore set out to use a model in vitro culture HeLa cell system to begin investigating the cold-shock response in mammalian cell systems. Sub-physiological temperature cultivation resulted in reduced growth and proliferation and a lower total cell protein content. Proteomic analysis confirmed that HeLa cells actively respond to sub-physiological temperature by upregulating a number of proteins and immunoblot analysis confirmed that specific proteins are indeed up-regulated in a time and temperature dependent manner. Additional work is likely to improve our understanding of the cold-shock response in mammalian cells and identify candidate target proteins for cell engineering to further enhance heterologous protein production at sub-physiological temperatures.

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Protein profile of the HeLa cell line

Cited by 35 publications

References 40 publications

Mechlorethamine-Induced DNA–Protein Cross-Linking in Human Fibrosarcoma (HT1080) Cells

Mechlorethamine-Induced DNA–Protein Cross-Linking in Human Fibrosarcoma (HT1080) Cells

Comparison of early passage, senescent and hTERT immortalized endothelial cells

The cold-shock response in mammalian cells: investigating the HeLa cell cold-shock proteome

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