Protein Profile of Human Lung Epithelial Cells (A549) Revealing Deviation in Cytoskeleton Proteins in Response to Zinc Oxide Nanoparticles Exposure

Abstract

Zinc oxide nanoparticles (ZnO NPs) are widely used in biomedicine and scientific research because of their high dissolution property and bioavailability. On the contrary, this property also increases the intracellular reactivity, accessibility and cytotoxicity. These nano-bio interactions could induce undesirable changes in the proteome of the interacting cells, especially in the lung cells as these are the primary contact site. However, the potential effects of ZnO NPs exposure on proteome remain unclear. Proteomics data will substantiate the detailed mechanism of cellular interactions and modulatory effects of ZnO NPs on cells. Quantitative proteomic profiling was done using MALDI-TOF/TOF and MS/MS to identify differential protein expression on exposure to NPs among non exposed and exposed cells. Twenty-two proteins, with approximately 1.5 fold differential expression in cells exposed to ZnO NPs as compared to control cells were identified. Differentially expressed proteins were further classified using PANTHER software on the basis of functional gene ontology term: molecular function, biological process and cellular component. ToppGene suite was used to study protein-protein interaction and network was enriched with STRING. This study is a systematic analysis of protein modulation of the A549 cells exposed to ZnO NPs indicating alterations in the cytoskeleton.

Zinc oxide nanoparticles trigger dysfunction of mitochondrial respiratory complexes and repair dynamics in human alveolar cells

“…The characterization of these NPs in the culture medium was performed through dynamic light scattering (DLS). The actual size and morphology of the particles were measured through transmission electron microscopy (TEM) (Anand et al, 2020; Jain et al, 2014). A microliter aliquot of NPs in Milli-Q water was added to a 300 mesh carbon-coated grid.…”

Phytoalexin and biomarker peptide detection using mass spectrometry

“…They also suggested that HSP70 play an important role for cellular motility, migration, and invasion. Increased expression of HSP70 was also found in A549 cells to protect them from hypoxic injury and to response to zinc oxide nanoparticles exposure (141,142). The exposure of cigarette smoke has been reported as another cause for inducing the up-regulated expression of HSP70 in rat brain (143).…”