Protein preparation, crystallization and preliminary X-ray analysis of<i>Polygonum cuspidatum</i>bifunctional chalcone synthase/benzalacetone synthase

Lu, Hongyu; Yang, Mo; Liu, Chunmei; Lu, Ping; Cang, Huaixing; Ma, Lanqing

doi:10.1107/s1744309113017004

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…There are many PKS super family genes, known as CHS-like genes, including genes encoding stilbene synthase (STS), 2-pyrone synthase (2-PS), benzalacetone synthase, biphenyl synthase, phlorisovalerophenone synthase, p-coumaroyltriacetic acid synthase, aloesone synthase, hexaketide synthase, pentaketide chromone synthase, etc. [21][22][23][24][25][26][27]. CHS-like genes differ from CHS in many aspects, such as their initial substrates, intermediate products and catalytic mechanisms, etc.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification, Characterization and Expression Analysis of Mango (Mangifera indica L.) chalcone synthase (CHS) Genes in Response to Light

Shi

Zhu

et al. 2022

Horticulturae

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Light is one the most important environmental factors regulating the accumulation of specific metabolites in plants, such as flavonoid. Chalcone synthase (CHS) is the key rate-limiting enzyme of the flavonoid biosynthesis pathway, while little is known about the CHS genes in mango (Mangifera indica L.). Therefore, the aim of the study was to obtain the genome-wide identification of the CHS genes in mango and screen the light-responsive family members. In this study, a total of 21 CHS genes were identified from the mango genome, and most of the members contained the Cys-His-Asn triad and the CHS/STS signature motif. Most of the MiCHS genes were located on chromosomes 2 and 4, and seven pairs of tandem duplication genes and five pairs of segmental duplication genes were detected among the MiCHS members. The expression of MiCHSs showed a tissue-specific pattern and was not correlated with the flavonoid or anthocyanin accumulation in cultivars with different peel colors. MiCHS4, MiCHS16 and MiCHS17 were found to respond to preharvest and postharvest UV-B plus visible light treatments, which exhibited no significant relationship with the light-responsive cis-acting element distribution in the promoter region. Our results are helpful and can be used to further study the function of MiCHS genes in light-induced flavonoid biosynthesis in mango.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification, Characterization and Expression Analysis of Mango (Mangifera indica L.) chalcone synthase (CHS) Genes in Response to Light

Shi

Zhu

et al. 2022

Horticulturae

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“…CHS is a representative of the CHS family. This family also includes other members such as biphenyl synthase [ 5 ], 2-pyrone synthase (2-PS) [ 6 ], benzalacetone synthase [ 7 ], stilbene synthase [ 8 ], bibenzyl synthase [ 9 ], benzophenone synthase [ 10 ], pentaketide chromone synthase [ 11 ], octaketide synthase [ 12 ] and p -coumaroyltriacetic acid synthase [ 13 ]. All these other enzymes have differences from CHS in many aspects, such as the choice of initial substrates, the number of condensation reactions, and the mechanisms by which the resulting intermediates are cyclized and aromatized.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification, Characterization and Expression Analysis of the Chalcone Synthase Family in Maize

Han

Ding

et al. 2016

IJMS

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Members of the chalcone synthase (CHS) family participate in the synthesis of a series of secondary metabolites in plants, fungi and bacteria. The metabolites play important roles in protecting land plants against various environmental stresses during the evolutionary process. Our research was conducted on comprehensive investigation of CHS genes in maize (Zea mays L.), including their phylogenetic relationships, gene structures, chromosomal locations and expression analysis. Fourteen CHS genes (ZmCHS01–14) were identified in the genome of maize, representing one of the largest numbers of CHS family members identified in one organism to date. The gene family was classified into four major classes (classes I–IV) based on their phylogenetic relationships. Most of them contained two exons and one intron. The 14 genes were unevenly located on six chromosomes. Two segmental duplication events were identified, which might contribute to the expansion of the maize CHS gene family to some extent. In addition, quantitative real-time PCR and microarray data analyses suggested that ZmCHS genes exhibited various expression patterns, indicating functional diversification of the ZmCHS genes. Our results will contribute to future studies of the complexity of the CHS gene family in maize and provide valuable information for the systematic analysis of the functions of the CHS gene family.

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“…The crystal structure of PcPKS1 from Polygonum cuspidatum (Japanese knotweed) was also recently solved, and studies showed PcPKS1 to have both CHS and BAS activities [47]. Unlike rhubarb BAS and the classical BAS PcPKS2, the 'gatekeeper' Phe215 remained in PcPKS1, thus suggesting a different catalytic mechanism in the bifunctional PcPKS1.…”

Section: Benzalacetone Synthasementioning

confidence: 99%

Exploiting the Biosynthetic Potential of Type III Polyketide Synthases

Lim

Yew

2016

Molecules

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Abstract:Polyketides are structurally and functionally diverse secondary metabolites that are biosynthesized by polyketide synthases (PKSs) using acyl-CoA precursors. Recent studies in the engineering and structural characterization of PKSs have facilitated the use of target enzymes as biocatalysts to produce novel functionally optimized polyketides. These compounds may serve as potential drug leads. This review summarizes the insights gained from research on type III PKSs, from the discovery of chalcone synthase in plants to novel PKSs in bacteria and fungi. To date, at least 15 families of type III PKSs have been characterized, highlighting the utility of PKSs in the development of natural product libraries for therapeutic development.

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Protein preparation, crystallization and preliminary X-ray analysis ofPolygonum cuspidatumbifunctional chalcone synthase/benzalacetone synthase

Cited by 4 publications

References 14 publications

Genome-Wide Identification, Characterization and Expression Analysis of Mango (Mangifera indica L.) chalcone synthase (CHS) Genes in Response to Light

Genome-Wide Identification, Characterization and Expression Analysis of Mango (Mangifera indica L.) chalcone synthase (CHS) Genes in Response to Light

Genome-Wide Identification, Characterization and Expression Analysis of the Chalcone Synthase Family in Maize

Exploiting the Biosynthetic Potential of Type III Polyketide Synthases

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