Protein/polysaccharide‐based scaffolds mimicking native extracellular matrix for cardiac tissue engineering applications

Rosellini, Elisabetta; Zhang, Yu Shrike; Migliori, Bianca; Barbani, Niccoletta; Lazzeri, Luigi; Shin, Su Ryon; Dokmeci, Mehmet R.; Cascone, Maria Grazia

doi:10.1002/jbm.a.36272

Cited by 91 publications

(97 citation statements)

References 60 publications

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“…To realize the desired 3D cellular processes, numerous 3D biomimetic scaffolds that incorporate different biochemical, mechanical, or architectural cues have been developed for cell cultures [12][13][14][15]. Benefiting from their 3D structural features, these scaffolds have found various applications, such as cell therapy, basic organ physiology, drug discovery, and tissue engineering [16][17][18]. However, because of their polydispersity and the inability to control the degree of connectivity between their pores, most of these scaffolds could only provide limited external surfaces for random cell enrichment and attachment [19,20].…”

Section: Introductionmentioning

confidence: 99%

Responsive Inverse Opal Scaffolds with Biomimetic Enrichment Capability for Cell Culture

et al. 2019

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Three-dimensional (3D) porous scaffolds have a demonstrated value for tissue engineering and regenerative medicine. Inspired by the predation processes of marine predators in nature, we present new photocontrolled shrinkable inverse opal graphene oxide (GO) hydrogel scaffolds for cell enrichment and 3D culture. The scaffolds with adjustable pore sizes and morphologies were created using a GO and N-isopropylacrylamide dispersed solution as a continuous phase of microfluidic emulsions for polymerizing and replicating. Because of the interconnected porous structures and the remotely controllable volume responsiveness of the scaffolds, the suspended cells could be enriched into the inner spaces of the scaffolds through predator-like swallowing and discharging processes. Hepatocyte cells concentrated in the scaffold pores could form denser 3D spheroids more quickly via the controlled compression force caused by the shrinking of the dynamic scaffolds. More importantly, with a program of scaffold enrichment with different cells, an unprecedented 3D multilayer coculture system of endothelial-cell-encapsulated hepatocytes and fibroblasts could be generated for applications such as liver-on-a-chip and bioartificial liver. It was demonstrated that the resultant multicellular system offered significant improvements in hepatic functions, such as albumin secretion, urea synthesis, and cytochrome P450 expression. These features of our scaffolds make them highly promising for the biomimetic construction of various physiological and pathophysiological 3D tissue models, which could be used for understanding tissue level biology and in vitro drug testing applications.

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Section: Introductionmentioning

confidence: 99%

Responsive Inverse Opal Scaffolds with Biomimetic Enrichment Capability for Cell Culture

et al. 2019

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“…The nanostructures demonstrated an improvement of biocompatibility, cell proliferation, and neovascularization. Rosellini et al mimicked the chemical composition and molecular interactions found in the native cardiac ECM with scaffolds made of alginate and collagen/gelatin material [121]. The alginate and gelatin scaffolds were superior biochemically and mechanically when compared to alginate and collagen scaffolds.…”

Section: Tissue Regenerationmentioning

confidence: 99%

Protein–Polysaccharide Composite Materials: Fabrication and Applications

et al. 2020

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Protein–polysaccharide composites have been known to show a wide range of applications in biomedical and green chemical fields. These composites have been fabricated into a variety of forms, such as films, fibers, particles, and gels, dependent upon their specific applications. Post treatments of these composites, such as enhancing chemical and physical changes, have been shown to favorably alter their structure and properties, allowing for specificity of medical treatments. Protein–polysaccharide composite materials introduce many opportunities to improve biological functions and contemporary technological functions. Current applications involving the replication of artificial tissues in tissue regeneration, wound therapy, effective drug delivery systems, and food colloids have benefited from protein–polysaccharide composite materials. Although there is limited research on the development of protein–polysaccharide composites, studies have proven their effectiveness and advantages amongst multiple fields. This review aims to provide insight on the elements of protein–polysaccharide complexes, how they are formed, and how they can be applied in modern material science and engineering.

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“…The formation of materials from various polysaccharides has been utilized in tissue engineering, the process of regeneration of damaged tissue or reconstruction of organs by adapting appropriate 3D microenvironments for cell adhesion, proliferation, followed by the formation of new tissues . By using polysaccharides as key components, or as additives, the material scaffolds can mimic parts of the natural extracellular matrices, which are mainly composed of proteoglycans that are long‐chain polysaccharides and fibrous proteins, thus allowing the material to host new cells and improve their survival . Rousseau and Gagnieu used periodate‐oxidized glycogen as a collagen crosslinker to prepare macroscopic films with defined crosslinking degrees for cellular adhesion and proliferation.…”

Section: Glycogen As a Building Block Of Nanostructured Materialsmentioning

confidence: 99%

Glycogen as a Building Block for Advanced Biological Materials

2019

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complex drug delivery systems that are challenging to scale up and therefore have limited clinical and commercial translation. Although numerous nanoparticles have been widely developed and used for various applications, including biomedical applications, [2] there is a growing interest in nontoxic and degradable alternatives to first-generation synthetic nanomaterials. Ideally the synthesis of such nanoparticles needs to be cost effective, simple, green, reproducible, scalable, and the constitutive building blocks of the nanoparticles should be nontoxic, functional, biodegradable, and available on a large scale. Naturally occurring nanoparticles could be such a valid alternative. These nanoparticles include intracellular structures, such as magnetosomes [3] and glycogen, and extracellular assemblies such as lipoproteins and viruses. [3] Among these, glycogen is the most abundant and versatile biological nanoparticle, which fulfils the requirements for the fabrication of nanostructured biomaterials. Glycogen is nature's prime nanoparticle that exists in most organisms, from bacteria and archaea to humans, [4,5] as a vital component of the cellular energy machinery. It is a highly branched polysaccharide comprising repeating units of glucose connected by linear α-d-(1,4) glycosidic linkages with α-d-(1,6) branching, structured into roughly spherical nanoparticles that have a high water solubility and molecular weight.The use of polysaccharides as components for advanced materials has been an attractive concept for decades. [6,7] A key motive is that polysaccharides, as a natural biomaterial, are endowed with a level of biodegradability and biocompatibility. In addition, they can be easily modified chemically or biochemically to produce functional derivatives, which are important for various therapeutic applications. [8] The structures of polysaccharides vary considerably but span from linear to highly branched polymers with molecular weights ranging from thousands to millions, composed of mono-or disaccharides, or short-chain oligomers bound together by glycosidic linkages. [9] There is a rich history of research on the use of polysaccharides in a range of therapeutic applications including: i) soft tissue engineering, [10,11] where the materials can be designed to mimic the mechanical properties of the extracellular matrix in the tissue; ii) as drug, protein, or gene delivery systems, [7,12,13] where polysaccharides have a large number of reactive groups [14] that allow for tunable properties [12] such as pH-responsiveness; [15] and iii) as nanoprobes for cellular Biological nanoparticles found in living systems possess distinct molecular architectures and diverse functions. Glycogen is a unique biological polysaccharide nanoparticle fabricated by nature through a bottom-up approach. The biocatalytic synthesis of glycogen has evolved over time to form a nanometer-sized dendrimer-like structure (20-150 nm) with a highly branched surface and a dense core. This makes glycogen markedly different from other natur...

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Protein/polysaccharide‐based scaffolds mimicking native extracellular matrix for cardiac tissue engineering applications

Cited by 91 publications

References 60 publications

Responsive Inverse Opal Scaffolds with Biomimetic Enrichment Capability for Cell Culture

Responsive Inverse Opal Scaffolds with Biomimetic Enrichment Capability for Cell Culture

Protein–Polysaccharide Composite Materials: Fabrication and Applications

Glycogen as a Building Block for Advanced Biological Materials

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