Protein–polyelectrolyte interactions

Kayitmazer, A. Basak; Seeman, Daniel; Minsky, Burcu Baykal; Dubin, Paul L.; Xu, Yisheng

doi:10.1039/c2sm27002a

Cited by 360 publications

(306 citation statements)

References 322 publications

(464 reference statements)

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“…Thus, it is clear that besides favourable electrostatic interactions between the charged patches of the proteins and the lipid bilayer that drive the protein-lipid binding there are other short range interactions (van der Waals, hydrogen bonds, hydrophobic interactions and salt bridges) that decrease the protein dissociation from the lipid bilayers. This is a typical behaviour observed for proteins and polyelectrolytes in general as profoundly revised recently by Kayitmazer et al 29 For FBS, on the other hand, leakage events were observable starting from 0.1% dilution for negatively charged GUVs and from 1% dilution for neutral GUVs (Table 2 and Fig. 1).…”

Section: Discussionsupporting

confidence: 82%

“…However, both BSA and hemoglobin possess a highly heterogeneous surface charge distribution, 16,17 that gives them a dipolar character with positive patches across the domains (for an extensive recent review on the effects of charge patches on protein interactions see ref. 29). These positive patches are likely to be attracted by the negatively charged head group of the lipids.…”

Section: Discussionmentioning

confidence: 99%

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Non-specific interactions between soluble proteins and lipids induce irreversible changes in the properties of lipid bilayers

et al. 2013

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Soluble proteins in the extracellular matrix experience a crowded environment. However, most of the biophysical studies performed to date have focused on protein concentrations within the dilute regime (well below the mM range). Here, we systematically studied the interaction of model cell membrane systems (giant unilamellar vesicles and supported lipid bilayers) with soluble globular proteins, bovine serum albumin, hemoglobin and lysozyme at physiologically relevant concentrations. To mimic the extracellular environment more closely, we also used fetal bovine serum as a good representative of a biomimetic protein mixture. We found that regardless of the protein used (and thus of their biological function), the interactions between a model cell membrane and these proteins are determined by their physico-chemical characteristics, mainly their dipolar character (or charged patches). In this paper we discuss the specificity and reversibility of these interactions and their potential implications on the living cells. In particular, we report initial evidence for an additional role of glycolipids in cell membranes: that of reducing the effects of non-specific adsorption of soluble proteins on the cell membrane.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Non-specific interactions between soluble proteins and lipids induce irreversible changes in the properties of lipid bilayers

et al. 2013

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“…Characterization of coacervate phase behavior is typically performed using methods that take advantage of the light scattered by these small droplets in solution, such as turbidity and/or light scattering. While rheology can be used to identify the advent of complex formation based on an increase in viscosity [124], optical methods have been more widely utilized because they are amenable for high-throughput analysis to quantify the impact of variables such as the charge stoichiometry of the mixture, the ionic strength and pH of the solution, the total concentration of macro-ions, the charge density, and for samples containing polyelectrolytes, the polymer molecular weight [1][2][3]17,69,.…”

Section: Connecting Coacervate Phase Behavior With Materials Dynamicsmentioning

confidence: 99%

“…Coacervation has also recently been implicated in the formation of various biological assemblies [1,[14][15][16]55,[63][64][65][66][67][68][69][70]. Across nearly all of these applications, the vast majority of studies have focused on understanding and characterizing the equilibrium phase behavior of these materials as a function of parameters such as the chemistry of the charged species, the charge stoichiometry of the system, ionic strength, and pH [1][2][3]17,69,. However, these types of equilibrium characterizations do not provide sufficient insight into the dynamic behavior of coacervates.…”

Section: Introductionmentioning

confidence: 99%

Linear viscoelasticity of complex coacervates

Liu

Winter

Perry

2017

Advances in Colloid and Interface Science

125

163

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Rheology is a powerful method for materials characterization that can provide detailed information about the self-assembly, structure, and intermolecular interactions present in a material. Here, we review the use of linear viscoelastic measurements for the rheological characterization of complex coacervate-based materials. Complex coacervation is an electrostatically and entropically-driven associative liquid-liquid phase separation phenomenon that can result in the formation of bulk liquid phases, or the self-assembly of hierarchical, microphase separated materials. We discuss the need to link thermodynamic studies of coacervation phase behavior with characterization of material dynamics, and provide parallel examples of how parameters such as charge stoichiometry, ionic strength, and polymer chain length impact self-assembly and material dynamics. We conclude by highlighting key areas of need in the field, and specifically call for the development of a mechanistic understanding of how molecular-level interactions in complex coacervate-based materials affect both selfassembly and material dynamics.

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“…While complex coacervate materials may be comprised of any number of component types, such as colloids or folded proteins, [42][43][44][45][46] we will primarily focus on flexible, charged polymers. These systems typically have four non-water components, two oppositely-charged polyions and two oppositely-charged salt ions.…”

Section: Introductionmentioning

confidence: 99%

PRISM-Based Theory of Complex Coacervation: Excluded Volume versus Chain Correlation

2015

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Aqueous solutions of oppositely-charged polyelectrolytes can undergo liquid-liquid phase separation into materials known as complex coacervates. These coacervates have been a subject of intense experimental and theoretical interest. Efforts to provide a physical description of complex coacervates have led to a number of theories that qualitatively (and sometimes quantitatively) agree with experimental data. However, this agreement often occurs in a degeneracy of models with profoundly different starting assumptions and different levels of sophistication. Theoretical difficulties in these systems are similar to those in most polyelectrolyte systems where charged species are highly correlated. These highly-correlated systems can be described using Liquid State (LS) integral equation theories, which surpass mean field theories by providing information * To whom correspondence should be addressed † University of Massachusetts Amherst ‡ University of Illinois Urbana-Champaign 1 on local charge ordering. We extend these ideas to complex coacervate systems using PRISM-type theories, and are able to capture effects not observable in traditional coacervate models, particularly connectivity and excluded volume effects. We can thus bridge two traditional but incommensurate theories meant to describe complex coacervates: the Voorn-Overbeek theory and counterion release. Importantly, we hypothesize that a cancellation of connectivity and excluded volume effects provides an explanation for the ability of Voorn-Overbeek theory to fit experimental data despite its well-known approximations.

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Protein–polyelectrolyte interactions

Cited by 360 publications

References 322 publications

Non-specific interactions between soluble proteins and lipids induce irreversible changes in the properties of lipid bilayers

Non-specific interactions between soluble proteins and lipids induce irreversible changes in the properties of lipid bilayers

Linear viscoelasticity of complex coacervates

PRISM-Based Theory of Complex Coacervation: Excluded Volume versus Chain Correlation

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