Protein phosphorylation and membrane association of sucrose synthase in developing tomato fruit

Anguenot, Raphaël; Nguyen‐Quoc, Binh; Yelle, Serge; Michaud, Dominique

doi:10.1016/j.plaphy.2006.06.009

Cited by 22 publications

(13 citation statements)

References 42 publications

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“…In this regard, the in vitro phosphorylation of the ZmSUS1 protein in maize, belonging to group I-2, was found to cause the release of SUS from the membrane fraction (Winter et al, 1997). In the developing tomato fruit, the subcellular localization of SUS isoforms has also been found to be developmentally controlled, the membrane form being specifically detected in actively growing fruits, depending on the phosphorylation of SUS proteins (Anguenot et al, 2006). It will thus be interesting to determine whether the phosphorylation/dephosphorylation of OsSUS5 and OsSUS7 alters their subcellular localization.…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of the Duplicate Rice Sucrose Synthase Genes OsSUS5 and OsSUS7 Which Are Associated with the Plasma Membrane

Cho

Kim

et al. 2011

Molecules and Cells

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Systematic searches using the complete genome sequence of rice (Oryza sativa) identified OsSUS7, a new member of the rice sucrose synthase (OsSUS) gene family, which shows only nine single nucleotide substitutions in the OsSUS5 coding sequence. Comparative genomic analysis revealed that the synteny between OsSUS5 and OsSUS7 is conserved, and that significant numbers of transposable elements are scattered at both loci. In particular, a 17.6-kb genomic region containing transposable elements was identified in the 5′ upstream sequence of the OsSUS7 gene. GFP fusion experiments indicated that Os-SUS5 and OsSUS7 are largely associated with the plasma membrane and partly with the cytosol in maize mesophyll protoplasts. RT-PCR analysis and transient expression assays revealed that OsSUS5 and OsSUS7 exhibit similar expression patterns in rice tissues, with the highest expression evident in roots. These results suggest that two redundant genes, OsSUS5 and OsSUS7, evolved via duplication of a chromosome region and through the transposition of transposable elements.

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Section: Discussionmentioning

confidence: 99%

Identification and Characterization of the Duplicate Rice Sucrose Synthase Genes OsSUS5 and OsSUS7 Which Are Associated with the Plasma Membrane

Cho

Kim

et al. 2011

Molecules and Cells

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“…Nevertheless, in these correlation analyses, one cannot rule out that posttranslational modifications or proteinprotein interactions modulate V max while the enzyme content remains constant. For instance, a Susy isoform has been shown to be phosphorylated and subsequently translocated to the plasma membrane in developing tomato fruit (Anguenot et al, 2006), raising the possibility that UDP-glucose could somehow be channeled into cell wall constituents (Winter and Huber, 2000). Moreover, acid invertases can be inhibited by proteinaceous inhibitors in tomato fruit (Tauzin et al, 2014), thus interfering in vivo with its development and hexose content (Jin et al, 2009).…”

Section: Inherent (Enzyme-based) Versus Hierarchical (Network-based) mentioning

confidence: 99%

Model-Assisted Analysis of Sugar Metabolism throughout Tomato Fruit Development Reveals Enzyme and Carrier Properties in Relation to Vacuole Expansion

et al. 2014

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A kinetic model combining enzyme activity measurements and subcellular compartmentation was parameterized to fit the sucrose, hexose, and glucose-6-P contents of pericarp throughout tomato (Solanum lycopersicum) fruit development. The model was further validated using independent data obtained from domesticated and wild tomato species and on transgenic lines. A hierarchical clustering analysis of the calculated fluxes and enzyme capacities together revealed stage-dependent features. Cell division was characterized by a high sucrolytic activity of the vacuole, whereas sucrose cleavage during expansion was sustained by both sucrose synthase and neutral invertase, associated with minimal futile cycling. Most importantly, a tight correlation between flux rate and enzyme capacity was found for fructokinase and PPi-dependent phosphofructokinase during cell division and for sucrose synthase, UDP-glucopyrophosphorylase, and phosphoglucomutase during expansion, thus suggesting an adaptation of enzyme abundance to metabolic needs. In contrast, for most enzymes, flux rates varied irrespectively of enzyme capacities, and most enzymes functioned at <5% of their maximal catalytic capacity. One of the major findings with the model was the high accumulation of soluble sugars within the vacuole together with organic acids, thus enabling the osmotic-driven vacuole expansion that was found during cell division.

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“…Soluble Sus has been described extensively in different plants (Winter and Huber, 2000) and may be involved in metabolic activities related to glycolysis and energy production. Soluble Sus exists in equilibrium with the membraneassociated form, as in tobacco (Matic et al, 2004), cotton (Gossypium hirsutum; Amor et al, 1995), tomato (Solanum lycopersicum) fruits (Anguenot et al, 2006), and maize (Carlson and Chourey, 1996). Plasma membrane-associated Sus probably provides metabolites for both callose synthase and cellulose synthase.…”

Section: Pollen Tubes Contain a Protein Biochemically And Immunologicmentioning

confidence: 99%

“…The second form of Sus is associated with plasma membranes or cell walls and is generally involved in the synthesis of cell wall components, providing metabolites (usually UDP-Glc) for callose synthase and cellulose synthase (Amor et al, 1995). The association of Sus with cell membranes may be regulated by phosphorylation/dephosphorylation processes (Anguenot et al, 2006). This model is supported by evidence obtained with cotton fibers (Ruan et al, 1997), in which mutants deficient in fiber synthesis showed altered levels of Sus and suppression of its gene activity inhibited fiber growth (Ruan et al, 2003).…”

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confidence: 99%

Sucrose Synthase Is Associated with the Cell Wall of Tobacco Pollen Tubes

et al. 2008

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Sucrose synthase (Sus; EC 2.4.1.13) is a key enzyme of sucrose metabolism in plant cells, providing carbon for respiration and for the synthesis of cell wall polymers and starch. Since Sus is important for plant cell growth, insights into its structure, localization, and features are useful for defining the relationships between nutrients, growth, and cell morphogenesis. We used the pollen tube of tobacco (Nicotiana tabacum) as a cell model to characterize the main features of Sus with regard to cell growth and cell wall synthesis. Apart from its role during sexual reproduction, the pollen tube is a typical tip-growing cell, and the proper construction of its cell wall is essential for correct shaping and direction of growth. The outer cell wall layer of pollen tubes consists of pectins, but the inner layer is composed of cellulose and callose; both polymers require metabolic precursors in the form of UDP-glucose, which is synthesized by Sus. We identified an 88-kD polypeptide in the soluble, plasma membrane and Golgi fraction of pollen tubes. The protein was also found in association with the cell wall. After purification, the protein showed an enzyme activity similar to that of maize (Zea mays) Sus. Distribution of Sus was affected by brefeldin A and depended on the nutrition status of the pollen tube, because an absence of metabolic sugars in the growth medium caused Sus to distribute differently during tube elongation. Analysis by bidimensional electrophoresis indicated that Sus exists as two isoforms, one of which is phosphorylated and more abundant in the cytoplasm and cell wall and the other of which is not phosphorylated and is specific to the plasma membrane. Results indicate that the protein has a role in the construction of the extracellular matrix and thus in the morphogenesis of pollen tubes.

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Protein phosphorylation and membrane association of sucrose synthase in developing tomato fruit

Cited by 22 publications

References 42 publications

Identification and Characterization of the Duplicate Rice Sucrose Synthase Genes OsSUS5 and OsSUS7 Which Are Associated with the Plasma Membrane

Identification and Characterization of the Duplicate Rice Sucrose Synthase Genes OsSUS5 and OsSUS7 Which Are Associated with the Plasma Membrane

Model-Assisted Analysis of Sugar Metabolism throughout Tomato Fruit Development Reveals Enzyme and Carrier Properties in Relation to Vacuole Expansion

Sucrose Synthase Is Associated with the Cell Wall of Tobacco Pollen Tubes

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