Protein phosphatase type 2C PP2CA together with ABI1 inhibits SnRK2.4 activity and regulates plant responses to salinity

Krzywińska, Ewa; Kulik, Anna; Bucholc, Maria; Fernández, María Á.; Rodriguez, Pedro L.; Dobrowolska, Grażyna

doi:10.1080/15592324.2016.1253647

Cited by 40 publications

(29 citation statements)

References 25 publications

(10 reference statements)

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“…SnRK2.4 and OST1 were comparably inhibited by the ABI1‐related subclass of ABA coreceptors including ABI2, HAB1, and HAB2 but SnRK2.4 was less efficiently regulated by PP2CA‐type phosphatases encompassing PP2CA, HAI1 to HAI3, and AHG1. The finding is consistent with the reported inhibition of SnRK2.4 by ABI1 and PP2CA (Krzywińska et al , ).…”

Section: Discussionsupporting

confidence: 93%

Rebuilding core abscisic acid signaling pathways of Arabidopsis in yeast

et al. 2019

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The phytohormone abscisic acid (ABA) regulates plant responses to abiotic stress, such as drought and high osmotic conditions. The multitude of functionally redundant components involved in ABA signaling poses a major challenge for elucidating individual contributions to the response selectivity and sensitivity of the pathway. Here, we reconstructed single ABA signaling pathways in yeast for combinatorial analysis of ABA receptors and coreceptors, downstream‐acting SnRK2 protein kinases, and transcription factors. The analysis shows that some ABA receptors stimulate the pathway even in the absence of ABA and that SnRK2s are major determinants of ABA responsiveness by differing in the ligand‐dependent control. Five SnRK2s, including SnRK2.4 known to be active under osmotic stress in plants, activated ABA‐responsive transcription factors and were regulated by ABA receptor complexes in yeast. In the plant tissue, SnRK2.4 and ABA receptors competed for coreceptor interaction in an ABA‐dependent manner consistent with a tight integration of SnRK2.4 into the ABA signaling pathway. The study establishes the suitability of the yeast system for the dissection of core signaling cascades and opens up future avenues of research on ligand‐receptor regulation.

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Section: Discussionsupporting

confidence: 93%

Rebuilding core abscisic acid signaling pathways of Arabidopsis in yeast

et al. 2019

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“…Overexpression of some PYL, PP2C, and SAPK2 genes significantly increases or decreases drought tolerance in transgenic plants [ 56 – 58 ]. Moreover, the ABA receptor PYL5 can inhibit the activity of clade A PP2Cs [ 59 ], and PP2CA together with ABI1 inhibits SnRK2.4 activity and regulates plant responses to salinity [ 60 ]. These data clarify the function and nature of the cascade relationship of these genes in the stress response of plants.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Expression Profiles of Hemp (Cannabis sativa L.) in Response to Drought Stress

Gao

Cheng

Zhao

et al. 2018

International Journal of Genomics

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Drought is the main environmental factor impairing hemp growth and yield. In order to decipher the molecular responses of hemp to drought stress, transcriptome changes of drought-stressed hemp (DS1 and DS2), compared to well-watered control hemp (CK1 and CK2), were studied with RNA-Seq technology. RNA-Seq generated 9.83, 11.30, 11.66, and 11.31 M clean reads in the CK1, CK2, DS1, and DS2 libraries, respectively. A total of 1292 differentially expressed genes (DEGs), including 409 (31.66%) upregulated and 883 (68.34%) downregulated genes, were identified. The expression patterns of 12 selected genes were validated by qRT-PCR, and the results were accordant with Illumina analysis. Gene Ontology (GO) and KEGG analysis illuminated particular important biological processes and pathways, which enriched many candidate genes such as NAC, B3, peroxidase, expansin, and inositol oxygenase that may play important roles in hemp tolerance to drought. Eleven KEGG pathways were significantly influenced, the most influenced being the plant hormone signal transduction pathway with 15 differentially expressed genes. A similar expression pattern of genes involved in the abscisic acid (ABA) pathway under drought, and ABA induction, suggested that ABA is important in the drought stress response of hemp. These findings provide useful insights into the drought stress regulatory mechanism in hemp.

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“…Salt stress also triggers abscisic acid (ABA) pathways and osmotic stress signaling. Salt treatment increases ABA concentrations in plant cells (Duan et al ., ; Geilfus et al ., ) and activates sucrose nonfermenting 1‐related protein kinase 2 (SnRK2) kinase activity (Krzywińska et al ., ), and salt treatment of a protein phosphatase 2c ( pp2c ) double mutant ( abi1‐2 pp2ca‐1 ) leads to higher SnRK2 kinase activity (Krzywińska et al ., ). However, some evidence also indicates that salt/osmotic stress‐mediated SnRK2 activation is independent of ABA signaling (Boudsocq et al ., ; Zhang H. et al ., 2011; Zhang et al ., ).…”

Section: Sensing Salt Stressmentioning

confidence: 99%

Elucidating the molecular mechanisms mediating plant salt‐stress responses

2017

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Contents Summary523I.Introduction523II.Sensing salt stress524III.Ion homeostasis regulation524IV.Metabolite and cell activity responses to salt stress527V.Conclusions and perspectives532Acknowledgements533References533 Summary Excess soluble salts in soil (saline soils) are harmful to most plants. Salt imposes osmotic, ionic, and secondary stresses on plants. Over the past two decades, many determinants of salt tolerance and their regulatory mechanisms have been identified and characterized using molecular genetics and genomics approaches. This review describes recent progress in deciphering the mechanisms controlling ion homeostasis, cell activity responses, and epigenetic regulation in plants under salt stress. Finally, we highlight research areas that require further research to reveal new determinants of salt tolerance in plants.

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Protein phosphatase type 2C PP2CA together with ABI1 inhibits SnRK2.4 activity and regulates plant responses to salinity

Cited by 40 publications

References 25 publications

Rebuilding core abscisic acid signaling pathways of Arabidopsis in yeast

Rebuilding core abscisic acid signaling pathways of Arabidopsis in yeast

Genome-Wide Expression Profiles of Hemp (Cannabis sativa L.) in Response to Drought Stress

Elucidating the molecular mechanisms mediating plant salt‐stress responses

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