Protein Phosphatase Inhibitors Enhance the Expression of an α-Amylase Gene, αAmy3, in Cultured Rice Cells

Lue, Ming-Yong; Lee, Hung-Tu

doi:10.1006/bbrc.1994.2737

Cited by 23 publications

(12 citation statements)

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“…OKA is capable of inducing specific genes at the transcriptional level in higher plants (Dominov et al, 1992;Raz and Fluhr, 1993;Gianfagna and Lawton, 1995;Lue and Lee, 1995;Rojo et al, 1998). In this study, we showed that OKA induced the accumulation of NADH-GOGAT mRNA (Fig.…”

Section: Discussionsupporting

confidence: 60%

Okadaic Acid Mimics Nitrogen-Stimulated Transcription of the NADH-Glutamate Synthase Gene in Rice Cell Cultures

Hirose

Yamaya

1999

Plant Physiol.

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Section: Discussionsupporting

confidence: 60%

Okadaic Acid Mimics Nitrogen-Stimulated Transcription of the NADH-Glutamate Synthase Gene in Rice Cell Cultures

Hirose

Yamaya

1999

Plant Physiol.

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“…In cultivated rice cells, the expression of α-amylase genes like RAmy3D is repressed by sugars (99). In this system, protein phosphatase inhibitors strongly induce amy3D expression and an AMP-activated protein kinase may be involved in induction.…”

Section: Cereal Seed Germinationmentioning

confidence: 94%

SUGAR-INDUCED SIGNAL TRANSDUCTION IN PLANTS

Smeekens

2000

Annu. Rev. Plant. Physiol. Plant. Mol. Biol.

677

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s Abstract Sugars have important signaling functions throughout all stages of the plant's life cycle. This review presents our current understanding of the different mechanisms of sugar sensing and sugar-induced signal transduction, including the experimental approaches used. In plants separate sensing systems are present for hexose and sucrose. Hexokinase-dependent and -independent hexose sensing systems can further be distinguished. There has been progress in understanding the signal transduction cascade by analyzing the function of the SNF1 kinase complex and the regulatory PRL1 protein. The role of sugar signaling in seed development and in seed germination is discussed, especially with respect to the various mechanisms by which sugar signaling controls gene expression. Finally, recent literature on interacting signal transduction cascades is discussed, with particular emphasis on the ethylene and ABA signal transduction pathways.

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“…In cultivated rice cells the expression of a-amylase genes such as amy3D is repressed by sugars (Lue and Lee, 1994). In this system protein phosphatase inhibitors strongly induce amy3D expression and an AMPactivated protein kinase may be involved in induction.…”

Section: How Is the Signal Transduced?mentioning

confidence: 97%

Sugar Sensing and Sugar-Mediated Signal Transduction in Plants

Smeekens

Rook²

1997

Plant Physiology

562

577

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Information concerning the sugar status of plant cells is of great importance during a11 stages of the plant life cycle. The availability of or lack of sugars triggers many metabolic and developmental responses, and it is not surprising, therefore, that sugars profoundly affect the expression of a large number of genes (for review, see Koch, 1996;Graham, 1996). Sugar sensing occurs at the level of individual cells and the responses of such cells must be integrated at the tissue, organ, and plant level. Therefore, sugar-induced signals will interact with other sensing and signaling pathways. The mechanisms used by plant cells to sense sugars and to process this information are essentially unknown, and only recently are these questions being addressed experimentally. This lack of knowledge contrasts with the situation in yeast and bacteria, in which the molecular and physiological analysis of mutants have yielded extensive information about sugar perception (Trumbly, 1992;Ronne, 1995;Saier et al., 1995). SUGAR SENSING IN YEAST A N D ANIMALSYeast (Sacckauomyces ceuevisiae) serves as a model for investigating many basic biological questions about eukaryotes and is also an important paradigm for sugar sensing in plants. In yeast the availability of the preferred sugar substrate Glc signals the Glc repression phenomenon (for review, see Trumbly, 1992;Ronne, 1995;Thevelein and Hohmann, 1995). Glc repression dramatically alters yeast intermediary carbohydrate metabolism such that only Glc is being used as a carbon source, despite the presence of other readily accessible carbon sources. Glc is converted into Glu-6-P by HXK and is further metabolized via glycolysis. Genes involved in the metabolism of other carbon substrates are switched off, as are genes encoding key steps in gluconeogenic metabolism. A number of yeast mutants that are impaired in aspects of the Glc repression phenomenon have been isolated and their analysis has provided insight into the complexity of sugar sensing and signaling pathways. From these studies it was concluded that the Glc-phosphorylating enzyme HXK2 is a major Glc sensor responsible for sus- tained Glc repression. HXK2 activity initiates a signal transduction pathway that involves a number of different gene products (Fig. 1) and results in the repression of a large set of genes. Thus, the entry of Glc into glycolytic metabolism as mediated by HXK2 is a key step in Glc sensing.In the repression pathway the function of two protein complexes has been elucidated. These are the GLC7 type 1 protein phosphatase complex (Tu and Carlson, 1995, and refs. therein) and the SSN6/TUP1 complex, which functions as a general repressor of transcription through modulation of chromatin structure. Binding of the SSN6/TUP1 complex to specific sites is directed by the DNA-binding protein MIG1, and in this way genes that contain MIG1-binding sites are repressed. Exactly how the HXK2, GLC7, and SSNG/TUPl /MIG1 complexes are connected is unknown. For example, in the repression pathway no substrates for the REG1 ...

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Protein Phosphatase Inhibitors Enhance the Expression of an α-Amylase Gene, αAmy3, in Cultured Rice Cells

Cited by 23 publications

References 0 publications

Okadaic Acid Mimics Nitrogen-Stimulated Transcription of the NADH-Glutamate Synthase Gene in Rice Cell Cultures

Okadaic Acid Mimics Nitrogen-Stimulated Transcription of the NADH-Glutamate Synthase Gene in Rice Cell Cultures

SUGAR-INDUCED SIGNAL TRANSDUCTION IN PLANTS

Sugar Sensing and Sugar-Mediated Signal Transduction in Plants

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