Protein Phosphatase Inhibition Assay for Detection of Microcystins in Lake Water and Microcystis Cultures.

Yao, Yasuko; Ujiie, Yumi; Watanabe, Masayuki; Yagi, Osami; Takamura, Yoshichika

doi:10.1264/jsme2.13.149

Cited by 1 publication

(1 citation statement)

References 30 publications

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“…Those values are lower than the values (1.40 µg/L and 0.90 µg/L, respectively) reported by Campàs et al [27] using colorimetric inhibition assay. The value is well correlated with the LD 50 value measured by mouse bioassays [37]. Thus, the PP2A inhibition assay could be a suitable alternative of mouse bioassay to evaluate MC-LR toxicity in water samples.…”

Section: Mc-lr Assaymentioning

confidence: 54%

Micro-droplet Hydrodynamic Voltammetry for the Determination of Microcystin-LR Based on Protein Phosphatase

Islam

Sazawa

Sugawara

et al. 2019

J. of Wat. & Envir. Tech.

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Microcystin-LR (MC-LR) is one of the most frequent and most lethal toxicants and a vital environmental pollutant due to its toxicity and persistence. It is hepatotoxins and have been shown to be potent tumor-promoters which pose a serious threat to human health in the form of chronic exposure through drinking water. In this study, a novel electrochemical method for the determination of MC-LR has been developed using protein phosphatase 2A (PP2A) enzyme inhibition. The rapid and simple determination of PP2A activity was accomplished by means of hydrodynamic voltammetry in a 50 µL micro-droplet with a rotating disk electrode (RDE). The chronoamperometric response were obtained from the electrochemical oxidation of p-aminophenol (PAP) following the enzymatic conversion of p-aminophenyl phosphate (PAPP) as a substrate. PP2A activity estimated by using PAPP substrate is affected by MC-LR, and this phenomenon decreases the current signal of PAP. The IC 50 value was calculated as 0.08 µg/L which is well below the World Health Organization (WHO) provisional guideline value for total MC-LR of 1 µg/L in drinking water. The enzymatic reaction using RDE is possible to perform using fewer chemicals in a rapid manner. Therefore, the method can be very attractive for MC contaminated drinking water routine assessment.

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Section: Mc-lr Assaymentioning

confidence: 54%

Micro-droplet Hydrodynamic Voltammetry for the Determination of Microcystin-LR Based on Protein Phosphatase

Islam

Sazawa

Sugawara

et al. 2019

J. of Wat. & Envir. Tech.

View full text Add to dashboard Cite

show abstract

Protein Phosphatase Inhibition Assay for Detection of Microcystins in Lake Water and Microcystis Cultures.

Abstract: The protein phosphatase inhibition assay was used to quantify low levels of microcystin in lake

Cited by 1 publication

References 30 publications

Micro-droplet Hydrodynamic Voltammetry for the Determination of Microcystin-LR Based on Protein Phosphatase

Micro-droplet Hydrodynamic Voltammetry for the Determination of Microcystin-LR Based on Protein Phosphatase

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