Protein permeation through poly(vinyl alcohol) hydrogel membranes

K, Burczak; Fujisato, Toshiya; Hatada, Motoyoshi; Ikada, Yoshito

doi:10.1016/0142-9612(94)90072-8

Cited by 139 publications

(82 citation statements)

References 15 publications

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“…It is unclear whether cross-linking results in more coating or a denser coating. There are some reports on the structure of PVA cross-link including the effect of cross-linking agent on the structure of PVA hydrogel, such as the study of Burczak et al [8]. They illustrated that the cross-linked PVA hydrogel consists of networks of macromolecular chains (macromolecular mesh).…”

Section: Effect Of Dynamic Coating Timementioning

confidence: 99%

Dynamically formed poly (vinyl alcohol) ultrafiltration membranes with good anti-fouling characteristics

2000

Additives for Polymers

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The anti-fouling poly (vinyl alcohol) TFC membranes were dynamically prepared when an aqueous solution containing poly (vinyl alcohol), cross-linking agents and additives passed through porous substrate membranes under definite pressure followed by heat treatment resulting in cross-linking reaction and drying. By means of dead-end filtration the poly (vinyl alcohol) solution deposited on the surface and entered the pores of porous substrate and thus an even poly (vinyl alcohol) gel layer was formed on both the external and internal surface. The effects of poly (vinyl alcohol) concentration, dynamic coating time, additive concentration, cross-linking agent concentration, curing time and support membrane on pure water flux and protein rejection of the resultant membranes were studied. Membrane morphology was characterized by SEM studies. The modified membranes were investigated in ultrafiltration experiments with pepsin to assess their resistance to fouling. The experimental results prove that modified membranes with an even poly (vinyl alcohol) hydrogel layer show dramatically high anti-fouling characteristics compared to inadequately modified and unmodified membranes. Moreover, it is possible that a series of dynamic membranes within broader molecular weight cut-off can be readily obtained by suitable control and combination of various preparative conditions.

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Section: Effect Of Dynamic Coating Timementioning

confidence: 99%

Dynamically formed poly (vinyl alcohol) ultrafiltration membranes with good anti-fouling characteristics

2000

Additives for Polymers

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“…Agarose (5%) immunoisolation membranes characterized by Iwata et al [8], showed signi®cant permeability to IgG and serum complement proteins, but still provided short term immunoisolation. Similarly, Burczak et al [9] found that poly vinyl alcohol (PVA) membranes with number average molecular weights of either 48 000 or 247 000 also resulted in IgG passage. Such studies indicate the variations inherent in polymeric membrane pore size and the dif®culties in preventing of the passage of immunoglobulins through such membranes.…”

Section: Current Membrane Technologymentioning

confidence: 99%

“…The ability of the membrane to allow diffusion of glucose and insulin while restricting the passage of IgG was studied in vitro by measuring relative concentrations of the desired molecule on both sides of the membrane using a mini-diffusion chamber and monitoring diffusion out of the actual biocapsule [9]. The mini-diffusion chamber was designed and machined out of transparent acrylic.…”

Section: Diffusion Of Glucose and Insulin Through Microfabricated Memmentioning

confidence: 99%

Characterization of micromachined silicon membranes for immunoisolation and bioseparation applications

Desai

Hansford

Ferrari

1999

Journal of Membrane Science

141

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The application of microfabrication technology to create precise separation and isolation membranes for biomedical applications is described. By utilizing fabrication techniques commonly employed in the microelectronics industry (MEMS), membranes can be fabricated with well-controlled and uniform pore sizes, allowing the optimization of membrane parameters for biomedical applications in cell immunoisolation and viral ®ltration. Using bulk and surface micromachining to create diffusion membranes, pore sizes down to 18 nm have been attained through deposition and subsequent etching of sacri®cial layers on silicon. Membranes were shown to be suf®ciently permeable to small biomolecules such as oxygen, glucose, and insulin, while excluding the passage of larger proteins such as immunoglobulin G (IgG). The semipermeability of microfabricated membranes, their biocompatibility, ease in sterilization, along with their thermal and chemical stability, may provide a signi®cant advantages for biomedical applications. Microfabrication technology may also be applied to other materials of interest for the development of highly controlled membranes. #

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“…The cross-linking reaction occurred when hydroxyl groups in the PVA and aldehyde groups in the GA underwent an acid-catalyzed acetalization process, producing acetal and hemiacetal bridges between polymer chains. This process is stochastic in nature and has already been shown to depend on factors such as polymer concentration and reactive group tacticity [15,29,30] . A reaction scheme is shown in Figure 1.…”

Section: Sample Analysismentioning

confidence: 99%

Time domain NMR evaluation of poly(vinyl alcohol) xerogels

2016

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SbstractPoly(vinyl alcohol) (PVA)-based chemically cross-linked xerogels, both neat and loaded with nanoparticulate hydrophilic silica (SiO 2 ), were obtained and characterized mainly through time domain NMR experiments (TD-NMR). Fourier-transform infrared (FT-IR) and wide angle X-ray diffraction (WAXD) analyses were employed as secondary methods. TD-NMR, through the interpretation of the spin-lattice relaxation constant values and related information, showed both cross-linking and nanoparticle influences on PVA matrix. SiO 2 does not interact chemically with the PVA chains, but has effect on its molecular mobility, as investigated via TD-NMR. Apparent energy of activation, spin-lattice time constant and size of spin domains in the sample have almost linear dependence with the degree of cross-linking of the PVA and are affected by the addition of SiO 2 . These three parameters were derived from a single set of TD-NMR experiments, which demonstrates the versatility of the technique for characterization of inorganic-organic hybrid xerogels, an important class of materials.

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Protein permeation through poly(vinyl alcohol) hydrogel membranes

Cited by 139 publications

References 15 publications

Dynamically formed poly (vinyl alcohol) ultrafiltration membranes with good anti-fouling characteristics

Dynamically formed poly (vinyl alcohol) ultrafiltration membranes with good anti-fouling characteristics

Characterization of micromachined silicon membranes for immunoisolation and bioseparation applications

Time domain NMR evaluation of poly(vinyl alcohol) xerogels

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