Protein Patterns in Response to Cold Stress on Clones of Olea Europaea L., Cv Leccino

Bartolini, G.; Monte, G. Di; Rea, Elvira; Toponi, M. A.

doi:10.17660/actahortic.1999.474.99

Cited by 5 publications

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“…Olive ( Olea europaea L.) is one of the most cultivated woody plants, due to its adaptability to different environmental conditions and also to the nutritional value of its fruit (Bartolini et al 1999). The olive tree is an evergreen plant that grows mostly between latitudes of 30 and 45° in both hemispheres.…”

Section: Introductionmentioning

confidence: 99%

Cold-hardiness of olive (Olea europaeaL.) cultivars in cold-acclimated and non-acclimated stages: seasonal alteration of antioxidative enzymes and dehydrin-like proteins

2008

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SUMMARYSeasonal patterns of antioxidative enzymes and proteins and their relations to cold-hardiness of nine olive (Olea europaeaL.) cultivars (Ascolona, Domat, Gemlik, Hojoblanca, Lecquest, Manzanilla, Meski, Samanli and Uslu) are documented in the current study. Fully expanded, uniformly sized leaves from 2-year-old shoots of the cultivars were collected from 20-year-old trees in cold-acclimated (CA, in January) and non-acclimated (NA, in July) stages. Leaf samples were exposed to low temperature at 4, −5, −10 and −20°C for 12 h to determine their cold-hardiness (LT50; assessed by electrolyte leakage). Cold-acclimation produced an increase in freezing tolerance of all cultivars (by lowering LT50). Domat and Lecquest were found to have the highest cold-hardiness among the nine cultivars investigated. Ascolona, Gemlik, Hojoblanca had moderate cold-hardiness, while Samanli, Meski, Uslu and Manzanilla were more sensitive. Activities of catalase (CAT: EC 1·11·1·6), ascorbate peroxidase (APX: EC 1·11·1·11) and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase significantly varied depending on the cold-acclimation stage and the cold-hardiness level of the cultivars. Activities of the three antioxidative enzymes and total soluble proteins (TSP) were higher in the CA stage than in the NA stage. Although no accumulation of major polypeptides, except a 23 kDa protein, was detected either in CA samples or NA samples by SDS-PAGE, anti-dehydrin immunoblots revealed that the 43 and 23 kDa polypeptides were detectable during cold-acclimation of olive cultivars. Accumulation of both 43 and 23 kDa dehydrin was significantly higher in the CA stage than in the NA stage in all cultivars. Accumulation of 43 kDa dehydrin was correlated with cold-hardiness of the cultivars, while 23 kDa dehydrin was considered as cultivar-dependent since its accumulation was not parallel to LT50values of the cultivars. Indeed, the tissues of cvs Domat, Lecquest, Ascolona, Hojoblanca and Gemlik were found to enhance the structural stability of cellular membranes in the CA stage by increasing both the activity of such enzymes as CAT, APX and NADPH oxidase to activate the antioxidative systems and the expression of 43 kDa dehydrins.

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Section: Introductionmentioning

confidence: 99%

Cold-hardiness of olive (Olea europaeaL.) cultivars in cold-acclimated and non-acclimated stages: seasonal alteration of antioxidative enzymes and dehydrin-like proteins

2008

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“…In addition, knowledge of key proteins that play a crucial role in the development of a plant has the potential to complement metabolomics to elucidate the biochemistry of the phenotype being expressed (Deshmukh et al, 2014). There are many examples of proteomics-based studies that include the investigation of biotic and abiotic stresses, which negatively affect crop growth and productivity worldwide (Hashiguchi et al, 2010;Bartolini et al, 1999). Proteomics approaches are also useful to explore the molecular mechanisms underpinning plant-microbe interactions (Lodha et al, 2013;Quirino et al, 2010;Mehta et al, 2008).…”

Section: Snpmentioning

confidence: 99%

“…High throughput proteomics technologies coupled with advanced bioinformatics are increasingly leveraged to identify molecular signatures of economic importance based on protein pathways and signalling cascades. Bartolini et al (1999) Olive…”

Section: Technological Perspectives For Proteomics and Metabolomicsmentioning

confidence: 99%

Omics Platform Technologies for Discovery and Understanding the Systems Biology of Oil Palm

Ramli¹

2020

JOPR

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Palm oil is the leading vegetable oil in terms of volume in the world market. Indonesia and Malaysia are the top producers and exporters of the commodity. The global production of palm oil reached 73.5 million tonnes in the period 2018/2019, up from approximately 70.5 million tonnes in 2017/2018. Oil palm is the most productive crop in the world being 10 times more productive than soyabean which produces only about 0.45 t oil per hectare. Nonetheless, the industry is continuously under pressure to improve productivity and sustainability. This will require concerted innovations across the entire palm oil supply chain and fully committed research efforts including upstream technologies to expedite crop improvement. At the Malaysian Palm Oil Board (MPOB), research efforts at development of tools for improving oil palm traits by genetic modification have been augmented more recently with omics-based approaches and all of these innovations are synchronised towards improved product quality. Omics is a multidisciplinary field encompassing genomics, epigenomics, transcriptomics, proteomics and metabolomics. Each of these fields provides an opportunity to understand and view oil palm biology from a global perspective, enabling accelerated discoveries for improved productivity and the development of new and improved varieties. An integrative omics approach promises great value in both phenotyping and diagnostic analyses. With the current technological capabilities, metabolomics is also being exploited for identifying unique chemical fingerprints to detect product contamination and adulteration in oil palm. This effort is actively being conducted in order to position the oil palm industry to meet and optimise the delivery of the highest quality oil with minimum environmental and social concerns. In this review, an overview is given on the current knowledge and progress made in oil palm research, focusing on the application of omics strategies and their integration with high-throughput technologies for oil palm crop improvement, development of geographical traceability system and ensuring that palm oil is free from residual oil contamination.

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“…Although olive cultivars have the genetic potential to tolerate freezing, the degree of resistance in the existing genotypes is not enough to ensure an effective protection against severe frost events. To improve olive freezing resistance, the physiological and biochemical mechanisms involved in olive cold acclimation must be studied Bartolini et al 1999;.…”

Section: Low (Freezing) Temperaturesmentioning

confidence: 99%

Physiological and Biochemical Remarks on Environmental Stress in Olive (Olea Europaea L.)

Vitagliano¹,

Sebastiani²

2002

Acta Hortic.

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Olive (Olea europaea L.) is an evergreen tree traditionally cultivated in the Mediterranean basin where plants undergo, to some degree, stress due to unfavourable environmental conditions. Water deficit, freezing, salinity and air pollution are a few of the stress factors restricting growth, so that olive productivity at the end of the growing season expresses only a fraction of the plant's genetic potential. Understanding the physiological and biochemical processes that enable olive adaptation and acclimation, as well as the mechanisms of stress injury, is therefore of relevant importance. However, the studies on the physiology and biochemistry of stress resistance and on the role of gene expression and protein synthesis in olive are almost in an initial phase when compared with the progress achieved for other cultivated plants. In this paper, the physiological and biochemical studies performed on olive plants exposed to the main environmental stresses will be analysed and discussed. WATER DEFICIT In Mediterranean climates water deficit is probably one of the main restrictions for olive cultivation, though olive behaves as an intermediate drought-tolerant species when compared with other temperate fruit trees. Olive leaves can, in fact, tolerate very low water potential (up to-6÷8 MPa) and lose almost 40% of tissue water, while maintaining a full rehydration capacity (Rhizopoulou et al. 1991). These adaptations enable olive

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Protein Patterns in Response to Cold Stress on Clones of Olea Europaea L., Cv Leccino

Cited by 5 publications

References 0 publications

Cold-hardiness of olive (Olea europaeaL.) cultivars in cold-acclimated and non-acclimated stages: seasonal alteration of antioxidative enzymes and dehydrin-like proteins

Cold-hardiness of olive (Olea europaeaL.) cultivars in cold-acclimated and non-acclimated stages: seasonal alteration of antioxidative enzymes and dehydrin-like proteins

Omics Platform Technologies for Discovery and Understanding the Systems Biology of Oil Palm

Physiological and Biochemical Remarks on Environmental Stress in Olive (Olea Europaea L.)

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