Protein patterning by a DNA origami framework

Aslan, Hüsnü; Krissanaprasit, Abhichart; Besenbacher, Flemming; Gothelf, Kurt V.; Dong, Mingdong

doi:10.1039/c6nr03199d

Cited by 12 publications

(10 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, proteins may have a relatively weak interaction with our origami device because there is seldom empty space available for protein filling. For example, the study reported by Aslan et al indicated that the unfolded part of DNA can bind to the positively charged portion of BSA (Bovine Serum Albumin). Therefore, the mechanical stability, toxicity, and function of our origami device in cells require additional related assays.…”

Section: Resultsmentioning

confidence: 99%

A DNA Origami Mechanical Device for the Regulation of Microcosmic Structural Rigidity

Wan

Hong

Wang

et al. 2017

Small

View full text Add to dashboard Cite

DNA origami makes it feasible to fabricate a tremendous number of DNA nanostructures with various geometries, dimensions, and functionalities. Moreover, an increasing amount of research on DNA nanostructures is focused on biological and biomedical applications. Here, the reversible regulation of microcosmic structural rigidity is accomplished using a DNA origami device in vitro. The designed DNA origami monomer is composed of an internal central axis and an external sliding tube. Due to the external tube sliding, the device transforms between flexible and rigid states. By transporting the device into the liposome, the conformational change of the origami device induces a structural change in the liposome. The results obtained demonstrate that the programmed DNA origami device can be applied to regulate the microcosmic structural rigidity of liposomes. Because microcosmic structural rigidity is important to cell proliferation and function, the results obtained potentially provide a foundation for the regulation of cell microcosmic structural rigidity using DNA nanostructures.

show abstract

Section: Resultsmentioning

confidence: 99%

A DNA Origami Mechanical Device for the Regulation of Microcosmic Structural Rigidity

Wan

Hong

Wang

et al. 2017

Small

View full text Add to dashboard Cite

show abstract

“…DNA origami nanostructures are also increasingly employed in various materials science applications. Many studies have used DNA origami nanostructures as templates or lithography masks in order to transfer their shapes into other biological [ 22 , [91] , [92] , [93] ], organic [ [94] , [95] , [96] , [97] ], and especially inorganic materials [ 21 , 23 , 29 , 30 , [98] , [99] , [100] , [101] ]. Even if this requires conditions that deviate from the usual solution conditions, the shapes of the DNA origami templates are often transferred in a single processing step, so that their structural stability is usually of little concern.…”

Section: Materials Science Applicationsmentioning

confidence: 99%

Structural stability of DNA origami nanostructures under application-specific conditions

Ramakrishnan

Ijäs

Linko

et al. 2018

Computational and Structural Biotechnology Journal

146

124

View full text Add to dashboard Cite

With the introduction of the DNA origami technique, it became possible to rapidly synthesize almost arbitrarily shaped molecular nanostructures at nearly stoichiometric yields. The technique furthermore provides absolute addressability in the sub-nm range, rendering DNA origami nanostructures highly attractive substrates for the controlled arrangement of functional species such as proteins, dyes, and nanoparticles. Consequently, DNAorigami nanostructures have found applications in numerous areas of fundamental and applied research, ranging from drug delivery to biosensing to plasmonics to inorganic materials synthesis. Since many of those applications rely on structurally intact, well-definedDNA origami shapes, the issue of DNA origami stability under numerous application-relevant environmental conditions has received increasing interest in the past few years. In this mini-review we discuss the structural stability, denaturation, and degradation of DNA origami nanostructures under different conditions relevant to the fields of biophysics and biochemistry, biomedicine, and materials science, and the methods to improve their stability for desired applications.

show abstract

“…The production of functional crystalline frameworks is arguably the ultimate goal of DNA nanotechnology, and surely one of its most applicable outcomes. 1,2 Methodologies have been developed to create a variety of DNA-based arrays with high spatial resolution in 1D [3][4][5][6] and 2D, [6][7][8][9][10][11][12][13][14][15][16] and a number of routes exists to extend geometrical control to the third dimension. 6,[15][16][17][18][19][20] None of the available approaches, however, has been able provide a general route for the preparation of 3D DNA frameworks that combine high porosity, embedded functionality, robustness to design changes, and the ability to retain local order over large lengthscales.…”

Section: Introductionmentioning

confidence: 99%

Amphiphilic-DNA Platform for the Design of Crystalline Frameworks with Programmable Structure and Functionality

et al. 2018

View full text Add to dashboard Cite

The reliable preparation of functional, ordered, nanostructured frameworks would be a game changer for many emerging technologies, from energy storage to nanomedicine. Underpinned by the excellent molecular recognition of nucleic acids, along with their facile synthesis and breadth of available functionalizations, DNA Nanotechnology is widely acknowledged as a prime route for the rational design of nanostructured materials. Yet, the preparation of crystalline DNA frameworks with programmable structure and functionality remains a challenge. Here we demonstrate the potential of simple amphiphilic DNA motifs, dubbed C-stars, as a versatile platform for the design of programmable DNA crystals. In contrast to all-DNA materials, in which structure depends on the precise molecular details of individual building blocks, the self-assembly of C-stars is controlled uniquely by their topology and symmetry. Exploiting this robust self-assembly principle we design a range of topologically identical, but structurally and chemically distinct C-stars that following a one-pot reaction selfassemble into highly porous, functional, crystalline frameworks. Simple design variations allow us to fine-tune the lattice parameter and thus control the partitioning of macromolecules within the frameworks, embed responsive motifs that can induce isothermal disassembly, and include chemical moieties to capture target proteins specifically and reversibly.

show abstract

Protein patterning by a DNA origami framework

Cited by 12 publications

References 75 publications

A DNA Origami Mechanical Device for the Regulation of Microcosmic Structural Rigidity

A DNA Origami Mechanical Device for the Regulation of Microcosmic Structural Rigidity

Structural stability of DNA origami nanostructures under application-specific conditions

Amphiphilic-DNA Platform for the Design of Crystalline Frameworks with Programmable Structure and Functionality

Contact Info

Product

Resources

About