Protein Pattern Difference in the Colon Cancer Cell Lines Examined by Two-Dimensional Differential In-Gel Electrophoresis and Mass Spectrometry

Katayama, Masafumi; Nakano, Hiroshi; Ishiuchi, Atsuko; Wu, Wenwen; Oshima, Ryuji; Sakurai, Joe; Nishikawa, Hiroyuki; Yamaguchi, Susumu; Otsubo, Takehito

doi:10.1007/s00595-006-3301-y

Cited by 67 publications

(64 citation statements)

References 35 publications

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“…3). The SW480 CRC clone was characterized by a spreading epithelial-type morphology, although the SW620 clone displayed ovoid to elongated fibroblast-like morphology, indicating the cells maintained their original phenotypes and consistent with previous data (25,27,28). When grown in soft agar, the SW620 clone displayed enhanced growth manifested by higher colony formation ability than the SW480 clone (Fig.…”

Section: Discussionsupporting

confidence: 90%

O-Linked β-N-Acetylglucosaminylation (O-GlcNAcylation) in Primary and Metastatic Colorectal Cancer Clones and Effect of N-Acetyl-β-d-glucosaminidase Silencing on Cell Phenotype and Transcriptome

Yehezkel¹,

Cohen²,

Kliger³

et al. 2012

Journal of Biological Chemistry

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Section: Discussionsupporting

confidence: 90%

O-Linked β-N-Acetylglucosaminylation (O-GlcNAcylation) in Primary and Metastatic Colorectal Cancer Clones and Effect of N-Acetyl-β-d-glucosaminidase Silencing on Cell Phenotype and Transcriptome

Yehezkel¹,

Cohen²,

Kliger³

et al. 2012

Journal of Biological Chemistry

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“…Previous studies on protein expression report 9 differently expressed proteins in SW480 and SW620 cell lines [34]. Among reported proteins, we have confirmed a decreased expression of peroxiredoxin 2 (PRDX2) and an increased expression of alpha enolase (ENOA) in SW620 cells relative to SW480.…”

Section: Discussionsupporting

confidence: 73%

Death by UVC Light Correlates with Protein Damage in Isogenic Human Tumor Cells: Primary Tumor SW480 versus its Metastasis SW620

2015

J Proteomics Computational Biol

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A correlation between protein damage and death, but not DNA damage, was found among cells from robust and standard bacterial and invertebrate species. However, the bottleneck in DNA repair efficacy appears more at the level of proteome damage than DNA damage. Here we present a comparative study of SW480 cells derived from a primary colon adenocarcinoma and SW620 metastatic cells derived from the same primary tumor and provide evidence that correlation between death and proteome damage extends to human tumor cells. A higher resistance of SW620 cells, compared to SW480, to killing by UVC light correlates with reduced levels of incurred irreversible oxidative protein damage (carbonylation) related to lower levels of ROS and of proteins intrinsically susceptible to oxidative damage due to imperfect folding. This study provides a concept for sensitizing tumor cells to cancer therapies and assessment of cancer cell fitness.

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“…Many different proteins are expressed in SW480 and SW620 cell lines (19). In the current study, we selected 4 tumor biomarkers-HSP27, integrin b 3, VEGFR2, and Ki67-known to relate to tumor differentiation, invasion, angiogenesis, and proliferation, respectively (20)(21)(22)(23).…”

Section: Discussionmentioning

confidence: 99%

Using Dual-Tracer PET to Predict the Biologic Behavior of Human Colorectal Cancer

Wang

Zhang²,

Tian³

et al. 2009

J Nucl Med

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18 F-FDG and 39-deoxy-39-18 F-fluorothymidine ( 18 F-FLT) have been proven useful in diagnosing and staging many types of cancer but with emphasis on different aspects of tumor biology. The aim of the current study was to evaluate whether 18 F-FDG and 18 F-FLT can be used complementarily in monitoring the biologic characteristics of colorectal cancer (CRC). Methods: Human CRC cell lines SW480 and SW620 of the same genetic origin but different metastatic potential were cultured and implanted into nude mice to create CRC models. Uptake of 18 F-FDG and 18 F-FLT in SW480 and SW620 cells in vitro was assessed after incubation with radiotracers for 0, 30, 60, 90, and 120 min. In vivo imaging of SW480 and SW620 tumor-bearing mice was performed using small-animal PET/CT at 60 min after injection of each tracer. A region of interest was drawn over tumor and background to calculate the tumor-to-nontumor ratio (T/NT) using software on reconstructed images. Tumor growth rate, metastatic status, and survival time were assessed in tumor-bearing mice. The relationship between uptake of the tracers, metastatic capability, and tumor marker expression was evaluated using linear regression. Results: SW480 tumors grew more quickly than SW620 tumors (t 5 3.332, P 5 0.004). A higher incidence of lung and liver metastases was noted for SW480 than for SW620 (P 5 0.023). Uptake in SW480 and SW620 cells was significantly different between 18 F-FDG and 18 F-FLT (t 5 2.507, P 5 0.021, vs. t 5 3.497, P 5 0.002). In the small-animal PET study, the T/NT of 18 F-FDG did not differ between SW480 and SW620 tumors (2.69 6 0.98 vs. 3.09 6 1.26, P 5 0.524), but the T/NT of 18 F-FLT differed significantly between SW480 and SW620 tumors (3.65 6 0.51 vs. 2.22 6 0.42, P , 0.001). Heat shock protein 27 (HSP27) expression and integrin b 3 expression were higher, whereas vascular endothelial growth factor receptor 2 (VEGFR2) expression and Ki67 expression were lower, in SW480 cells than in SW620 cells. For SW480, metastases in lung and liver correlated significantly with 18 F-FLT uptake in tumors (r 5 0.763, P 5 0.005) and with expression of HSP27 (r 5 0.894, P 5 0.008) and integrin b 3 (r 5 0.635, P 5 0.088). A correlation was also found between 18 F-FLT uptake and expression of HSP27 (r 5 0.924, P 5 0.004) and integrin b 3 (r 5 0.813, P 5 0.025). No correlation was found between 18 F-FDG uptake in tumors and metastasis in lung and liver (r 5 20.111, P 5 0.388). However, there was a significantly negative correlation between 18 F-FDG uptake and the survival time of tumor-bearing mice (r 5 20.500, P 5 0.017), to which 18 F-FLT did not relate (r 5 0.262, P 5 0.182). Conclusion: High uptake of 18 F-FDG and 18 F-FLT may reflect poorer survival and a higher metastatic potential for CRC in mice. Combining 18 F-FDG with 18 F-FLT PET would be helpful in better predicting the biologic behavior of CRC.

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Protein Pattern Difference in the Colon Cancer Cell Lines Examined by Two-Dimensional Differential In-Gel Electrophoresis and Mass Spectrometry

Cited by 67 publications

References 35 publications

O-Linked β-N-Acetylglucosaminylation (O-GlcNAcylation) in Primary and Metastatic Colorectal Cancer Clones and Effect of N-Acetyl-β-d-glucosaminidase Silencing on Cell Phenotype and Transcriptome

O-Linked β-N-Acetylglucosaminylation (O-GlcNAcylation) in Primary and Metastatic Colorectal Cancer Clones and Effect of N-Acetyl-β-d-glucosaminidase Silencing on Cell Phenotype and Transcriptome

Death by UVC Light Correlates with Protein Damage in Isogenic Human Tumor Cells: Primary Tumor SW480 versus its Metastasis SW620

Using Dual-Tracer PET to Predict the Biologic Behavior of Human Colorectal Cancer

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