Protein partitioning in PEG/dextran aqueous two‐phase systems

Diamond, Alan D.; Hsu, Jong-Ping

doi:10.1002/aic.690360707

Cited by 97 publications

(53 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, we selected dextran as the hydrophilic segment to prepare an amphiphilic diblock polymer. Dextran is widely used as blood substitution, drug carriers and aqueous phase material for protein purification because its definite biocompatibility, biodegradability, hydrophilicity and high affinity to bio-macromolecules [22,23]. In addition, dextran possesses multiple hydroxyls which are convenient for chemical modification to endow polymersome surfaces with various desired functions such as drug-targeting and diagnostic recognition.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and self-assembly behavior of amphiphilic diblock copolymer dextran-block-poly(ε-caprolactone) (DEX-b-PCL) in aqueous media

Zhang¹,

Dou²,

Jin³

2010

Express Polym. Lett.

View full text Add to dashboard Cite

Abstract. An amphiphilic diblock copolymer, dextran-block-poly(ε-caprolactone) (DEX-b-PCL), with a series of welldefined chain lengths of each block was prepared by conjugating a dextran chain with a PCL block via aza-Michael addition reaction under mild conditions. For the dextran block, samples with relatively uniform molecular weight, 3.5 and 6.0 kDa, were used, and the PCL blocks were prepared via ring-opening polymerization at defined ratios of ε-caprolactone to initiator in order to give copolymers with mass fraction of dextran (fDEX) ranging from 0.16 to 0.45. When these copolymers were allowed to self-assemble in aqueous solution, the morphology of assembled aggregates varied as a function of fDEX when characterized by transmission electron microscope (TEM), fluorescence microscope (FM) and dynamic laser scattering (DLS). As fDEX decreases gradually from 0.45 to 0.16, the morphology of the copolymer assembly changes from spherical micelles to worm-like micelles and eventually to polymersomes, together with an increase in particle sizes.

show abstract

Section: Introductionmentioning

confidence: 99%

Synthesis and self-assembly behavior of amphiphilic diblock copolymer dextran-block-poly(ε-caprolactone) (DEX-b-PCL) in aqueous media

Zhang¹,

Dou²,

Jin³

2010

Express Polym. Lett.

View full text Add to dashboard Cite

show abstract

“…[4][5][6][7] In the synthesis of biomaterials, such as protein microspheres and hydrogel beads, organic solvents are usually involved. Upon solidifying the dispersed phase to form solid materials, organic solvents must be extracted by repeated washing.…”

Section: Introductionmentioning

confidence: 99%

All-aqueous multiphase microfluidics

Song¹,

Sauret²,

Shum³

2013

Biomicrofluidics

104

View full text Add to dashboard Cite

Immiscible aqueous phases, formed by dissolving incompatible solutes in water, have been used in green chemical synthesis, molecular extraction and mimicking of cellular cytoplasm. Recently, a microfluidic approach has been introduced to generate all-aqueous emulsions and jets based on these immiscible aqueous phases; due to their biocompatibility, these all-aqueous structures have shown great promises as templates for fabricating biomaterials. The physico-chemical nature of interfaces between two immiscible aqueous phases leads to unique interfacial properties, such as an ultra-low interfacial tension. Strategies to manipulate components and direct their assembly at these interfaces needs to be explored. In this paper, we review progress on the topic over the past few years, with a focus on the fabrication and stabilization of all-aqueous structures in a multiphase microfluidic platform. We also discuss future efforts needed from the perspectives of fluidic physics, materials engineering, and biology for fulfilling potential applications ranging from materials fabrication to biomedical engineering.

show abstract

“…The ATPS formed by these inexpensive and biocompatible polymers was originally described for the purification of biomolecules by way of molecular partitioning 2,10 . Partitioning occurs when additional molecules or particles that do not contribute to the phase system are mixed with PEG and DEX.…”

Section: Introductionmentioning

confidence: 99%

Cell Co-culture Patterning Using Aqueous Two-phase Systems

Frampton

White

Abraham

et al. 2013

JoVE

View full text Add to dashboard Cite

Cell patterning technologies that are fast, easy to use and affordable will be required for the future development of high throughput cell assays, platforms for studying cell-cell interactions and tissue engineered systems. This detailed protocol describes a method for generating co-cultures of cells using biocompatible solutions of dextran (DEX) and polyethylene glycol (PEG) that phase-separate when combined above threshold concentrations. Cells can be patterned in a variety of configurations using this method. Cell exclusion patterning can be performed by printing droplets of DEX on a substrate and covering them with a solution of PEG containing cells. The interfacial tension formed between the two polymer solutions causes cells to fall around the outside of the DEX droplet and form a circular clearing that can be used for migration assays. Cell islands can be patterned by dispensing a cell-rich DEX phase into a PEG solution or by covering the DEX droplet with a solution of PEG. Co-cultures can be formed directly by combining cell exclusion with DEX island patterning. These methods are compatible with a variety of liquid handling approaches, including manual micropipetting, and can be used with virtually any adherent cell type. Video LinkThe video component of this article can be found at

show abstract

Protein partitioning in PEG/dextran aqueous two‐phase systems

Cited by 97 publications

References 30 publications

Synthesis and self-assembly behavior of amphiphilic diblock copolymer dextran-block-poly(ε-caprolactone) (DEX-b-PCL) in aqueous media

Synthesis and self-assembly behavior of amphiphilic diblock copolymer dextran-block-poly(ε-caprolactone) (DEX-b-PCL) in aqueous media

All-aqueous multiphase microfluidics

Cell Co-culture Patterning Using Aqueous Two-phase Systems

Contact Info

Product

Resources

About