Protein partitioning and transport in supported cationic acrylamide‐based hydrogels

Russell, Shawn M.; Belcher, Emily B.; Carta, Giorgio

doi:10.1002/aic.690490510

Cited by 26 publications

(42 citation statements)

References 31 publications

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“…14 [62]. The length of the capillary was over 5 mm, which assured semi-infinite conditions for the 8 hr duration of the experiment.…”

Section: Optical Microscopy With Slab-shaped Matricessupporting

confidence: 42%

“…The length of the capillary was over 5 mm, which assured semi-infinite conditions for the 8 hr duration of the experiment. The myoglobin solution concentration was 1 g/L which gives q*~400 mg/mL [62]. As seen in Fig. 14, the concentration profiles are diffuse and become broader as time elapses.…”

Section: Optical Microscopy With Slab-shaped Matricesmentioning

confidence: 38%

“…The diffuse nature of the profiles is consistent with a homogeneous or adsorbed-phase transport mechanism. As shown in [62], analysis of the profiles with Eq. (43) yields a fairly constant diffusivity D = (8 ± 2) 10 ±8 cm 2 /s.…”

Section: Optical Microscopy With Slab-shaped Matricesmentioning

confidence: 44%

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Protein Mass Transfer Kinetics in Ion Exchange Media: Measurements and Interpretations

2005

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The paper gives a summary of successful methods for measuring protein mass transfer kinetics in ion exchange matrices along with models needed to interpret experimental results. Both macroscopic methods (isocratic elution, gradient elution, batch adsorption, frontal analysis) and microscopic methods are considered. In all cases the main focus is the determination of the effective intraparticle diffusivity in order to permit a comparison of different stationary phases and provide a basis for predicting chromatographic process performance. Experimental results for representative systems are evaluated alongside the experimental and modeling aspects. Practical criteria for the selection of experimental conditions and the application of different models are discussed along with the advantages and disadvantages of the various approaches.

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“…14 [62]. The length of the capillary was over 5 mm, which assured semi-infinite conditions for the 8 hr duration of the experiment.…”

Section: Optical Microscopy With Slab-shaped Matricessupporting

confidence: 42%

Section: Optical Microscopy With Slab-shaped Matricesmentioning

confidence: 38%

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Protein Mass Transfer Kinetics in Ion Exchange Media: Measurements and Interpretations

2005

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“…This could also explain the high D e,app /D 0 -values. However, surface or homogeneous diffusion would predict smooth profiles, [13][14][15] quite unlike those observed experimentally in our case. A final possibility is the existence of parallel pore and surface diffusion mechanisms.…”

Section: Modelingmentioning

confidence: 48%

“…The latter approaches give not only rates but also information about the mechanism of transport and include confocal laser scanning microscopy (CLSM), [4][5][6][7][8][9][10] refractive index based microscopy, 11,12 and light microscopy using chromophoric proteins diffusing in slab-shaped matrices. [13][14][15] Each of these methods has advantages and disadvantages. CLSM allows measurements in transparent chromatographic beads using fluorescently labeled protein tracers.…”

Section: Introductionmentioning

confidence: 44%

Protein adsorption kinetics in charged agarose gels: Effect of agarose content and modeling

Schirmer

Carta

2008

AIChE Journal

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in Wiley InterScience (www.interscience.wiley.com).The adsorption kinetics of myoglobin in charged gels of varying agarose content have been measured macroscopically, through batch uptake experiments, and microscopically, using light microscopy with gels supported in microfluidics chips. The apparent effective pore diffusivities, determined by fitting either set of rate data to the shrinking core model, were greater than the free solution diffusivity and concentrationdependent. Moreover, the microscopically derived concentration profiles were qualitatively different from the predicted ones. Therefore, a new model taking into account an assumed favorable partitioning of the protein in the pore liquid is proposed to describe the adsorption kinetics. The new model yields effective pore diffusivities that are in approximate agreement with the values determined chromatographically under nonbinding conditions and with hindered diffusion theory. In addition, it predicts concentration profiles in the gel that are consistent with those observed microscopically. The overall increase in mass transfer is attributed to the favorable partitioning of the protein in the pores at low ionic strength, which results in a greater diffusional driving force.

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Adsorption Kinetics

2010

Protein Chromatography

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Protein partitioning and transport in supported cationic acrylamide‐based hydrogels

Cited by 26 publications

References 31 publications

Protein Mass Transfer Kinetics in Ion Exchange Media: Measurements and Interpretations

Protein Mass Transfer Kinetics in Ion Exchange Media: Measurements and Interpretations

Protein adsorption kinetics in charged agarose gels: Effect of agarose content and modeling

Adsorption Kinetics

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