Protein packaging in ionic liquid mixtures: an ecofriendly approach towards the improved stability of β-lactoglobulin in cholinium-based mixed ionic liquids

Abstract

<p>The present work demonstrates a pioneering approach for the packaging of β-LG with improved stability in the presence of aqueous solutions containing cholinium-based ionic liquid mixtures.</p>

Recent Advances in Ionic Liquids in Biomedicine

“…[160] And -lactoglobulin experiences structural changes when exposed to choline iodide, but those effects can be negated by the addition of choline dihydrogen phosphate. [161] ILs have also shown great promise in enzyme activity retention. Lysozyme and Lipase are two enzymes that have been explored extensively.…”

“…A majority of the papers reviewed in this section utilize either E. coli , [ 157 , 160 , 161 , 170 , 190 ] Staphylococcus aureus , [ 184 , 192 ] or both [ 153 , 154 , 155 , 156 , 158 , 162 , 165 , 166 , 168 , 169 , 171 , 173 , 174 , 176 , 183 , 184 ] to test IL/formulation bactericidal efficacy. The two bacterial strains are extremely clinically relevant, as these are both common bacterial infections in humans, and they E. coli and S. aureus represent two general categories of bacteria, Gram‐negative and Gram‐positive, respectively.…”

“…[160] And 𝛽-lactoglobulin experiences structural changes when exposed to choline iodide, but those effects can be negated by the addition of choline dihydrogen phosphate. [161] ILs have also shown great promise in enzyme activity retention. The formulations allowed for full enzyme activity even after heat treating at 120 °C, with the authors attributing the activity retention to the stabilization of Trp residues in the active site at the EG-SAIL interface.…”

“…[ 160 ] And β ‐lactoglobulin experiences structural changes when exposed to choline iodide, but those effects can be negated by the addition of choline dihydrogen phosphate. [ 161 ]…”

Absorption of the [bmim][Cl] Ionic Liquid in DMPC Lipid Bilayers across Their Gel, Ripple, and Fluid Phases

“… 35 , 40 − 45 In this context, several biochemical and chemical physics studies on the effects of ILs on proteins, biomembranes, saccharides, and cells have been carried out in the last decade. 46 , 47 It has been shown, for example, that ILs are able to stabilize proteins, 48 , 49 to either favor or inhibit the formation of amyloids, 50 , 51 to disrupt biomembranes, 52 − 55 and to kill bacteria and cancer cells at doses that are not lethal to healthy cells. 56 , 57 Among all of these studies, one of the major focuses has been devoted to the investigation of the interactions between ILs and model biomembranes, mimicked by lipid bilayers.…”