Protein Nitrogen Determination by Kjeldahl Digestion and Ion Chromatography

Wang, Hsiaoling; Pampati, Nagarani; McCormick, William; Bhattacharyya, Lokesh

doi:10.1016/j.xphs.2016.03.039

Cited by 83 publications

(44 citation statements)

References 8 publications

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“…The quantities were assessed using the Kjeldahl method [22], 3,5-dinitrosalicylic acid colorimetric estimation [23], phenol-sulfuric acid determination [23], the petroleum benzine extraction method, vanillin-glacial acetic acid and perchloric acid colorimetric spectrophotometry [24], the periodate oxidation method [25], an erinitrit-aluminium trichloride assay [23], a high-performance liquid chromatography analysis [23], and the ashing method [26], respectively.…”

Section: Methodsmentioning

confidence: 99%

Hepatoprotective Effects of Antrodia cinnamomea: The Modulation of Oxidative Stress Signaling in a Mouse Model of Alcohol‐Induced Acute Liver Injury

Liu

Wang

et al. 2017

Oxidative Medicine and Cellular Longevity

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In the present study, the components of A. cinnamomea (AC) mycelia were systematically analyzed. Subsequently, its hepatoprotective effects and the underlying mechanisms were explored using a mouse model of acute alcohol-induced liver injury. AC contained 25 types of fatty acid, 16 types of amino acid, 3 types of nucleotide, and 8 types of mineral. The hepatoprotective effects were observed after 2 weeks of AC treatment at doses of 75 mg/kg, 225 mg/kg, and 675 mg/kg in the mouse model. These effects were indicated by the changes in the levels of aspartate aminotransferase, alanine aminotransferase, several oxidation-related factors, and inflammatory cytokines in serum and/or liver samples. AC reduced the incidence rate of necrosis, inflammatory infiltration, fatty droplets formation, and cell apoptosis in liver detecting via histological and TUNEL assay. In addition, AC reduced the expression of cleaved caspase-3, -8, and -9 and the levels of phosphor-protein kinase B (Akt) and phosphor-nuclear factor-κB (NF-κB) in the liver samples. Collectively, AC-mediated hepatoprotective effects in a mouse model of acute alcohol-induced liver injury are the result of reduction in oxidative stress. This may be associated with Akt/NF-κB signaling. These results provide valuable evidence to support the use of A. cinnamomea as a functional food and/or medicine.

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Section: Methodsmentioning

confidence: 99%

Hepatoprotective Effects of Antrodia cinnamomea: The Modulation of Oxidative Stress Signaling in a Mouse Model of Alcohol‐Induced Acute Liver Injury

Liu

Wang

et al. 2017

Oxidative Medicine and Cellular Longevity

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“…As measured previously, AC contains 11.7% of total sugar, 30.6% of total protein, 30.1% of crude fat, 8.1% of triterpenoids, 5.4% of mannitol, 0.35% of flavonoids, and 0.16% of adenosine, which were measured using 3,5-dinitrosalicylic acid colorimetric assay [18], Kjeldahl method [19], petroleum benzene extraction method, vanillin-glacial acetic acid and perchloric acid colorimetric spectrophotometry [20], periodate oxidation method [21], erinitrit-aluminium trichloride assay [18], and high-performance liquid chromatography analysis [18], respectively.…”

Section: Methodsmentioning

confidence: 99%

Antifatigue Effects of Antrodia cinnamomea Cultured Mycelium via Modulation of Oxidative Stress Signaling in a Mouse Model

Liu

et al. 2017

BioMed Research International

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Antrodia cinnamomea, a folk medicinal mushroom, has numerous biological effects. In this study, we aim to assess whether the antifatigue effects of A. cinnamomea mycelia (AC) and its underlying mechanisms are related to oxidative stress signaling using behavioral mouse models and biochemical indices detection. Mice were orally treated with AC at doses of 0.1, 0.3, and 0.9 g/kg for three weeks. AC had no effect on the spontaneous activities of mice indicating its safety on central nervous system. Furthermore, results obtained from weight-loaded forced swimming test, rotary rod test, and exhausted running test confirmed that AC significantly enhanced exercise tolerance of mice. Biochemical indices levels showed that these effects were closely correlated with inhibiting the depletion of glycogen and adenosine triphosphate stores, regulating oxidative stress-related parameters (superoxide dismutase, glutathione peroxidase, reactive oxygen species, and malondialdehyde) in serum, skeletal muscle, and liver of mice. Moreover, the effects of AC may be related with its regulation on the activations of AMP-activated protein kinase, protein kinase B, and mammalian target of rapamycin in liver and skeletal muscle of mice. Altogether, our data suggest that the antifatigue properties of AC may be one such modulation mechanism via oxidative stress-related signaling in mice.

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“…Aer harvesting, the fruiting bodies were placed into a drying oven and dried to a constant weight at 50 C. The fruiting bodies (100 g) were then pulverized, sieved with a 200 mesh sieve, and stored at 4 C for further use. 9 Protein concentrations were determined using the Kjeldahl method (Kjeltec™ 8000, Foss, Hilleroed, Denmark) 19 and fatty acid contents were analyzed using a Soxhlet extractor system (Automatic Fats Analyzer, model 2050, Foss, Hilleroed, Denmark). Total sugar was also determined using the Watt and Merrill method and an ultravioletvisible spectrophotometer (model T6, New Century, Beijing General Instrument Co., Ltd., Beijing, China).…”

Section: Mushroom Cultivation and Preparationmentioning

confidence: 99%

Effects of mixed agro-residues (corn crop waste) on lignin-degrading enzyme activities, growth, and quality ofLentinula edodes

Wang

et al. 2020

RSC Adv.

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Protein Nitrogen Determination by Kjeldahl Digestion and Ion Chromatography

Cited by 83 publications

References 8 publications

Hepatoprotective Effects of Antrodia cinnamomea: The Modulation of Oxidative Stress Signaling in a Mouse Model of Alcohol‐Induced Acute Liver Injury

Hepatoprotective Effects of Antrodia cinnamomea: The Modulation of Oxidative Stress Signaling in a Mouse Model of Alcohol‐Induced Acute Liver Injury

Antifatigue Effects of Antrodia cinnamomea Cultured Mycelium via Modulation of Oxidative Stress Signaling in a Mouse Model

Effects of mixed agro-residues (corn crop waste) on lignin-degrading enzyme activities, growth, and quality ofLentinula edodes

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