Protein nitration induced by Hemin/NO: A complementary mechanism through the catalytic functions of hemin and NO-scavenging

Alsharabasy, Amir M.; Glynn, Sharon A.; Farràs, Pau; Pandit, Abhay

doi:10.1016/j.niox.2022.04.005

Cited by 13 publications

(29 citation statements)

References 87 publications

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“…The calculated area under each curve is shown in Figure C, and the corresponding changes in the voltage readings are shown in Figures S2A and B. Similar inhibitory effects for • NO release from DETA-NO were reported when the polarization solution contained the BSA or FBS-containing culture medium . However, it is still unclear how the components of the testing solution affect the degradation of the NO-releasing NONOate compounds.…”

Section: Resultsmentioning

confidence: 53%

“…Similar inhibitory effects for • NO release from DETA-NO were reported when the polarization solution contained the BSA or FBS-containing culture medium. 37 However, it is still unclear how the components of the testing solution affect the degradation of the NO-releasing NONOate compounds. Interestingly, the effects of DMTMM-activated HA on the release of • NO from DETA-NO were less than those of the native HA.…”

Section: Resultsmentioning

confidence: 99%

“…A comparison of • NO release from 30 and 300 μM DETA-NO in phosphate buffer and the FBS-containing medium showed a degradation rate of the NO-donor in the former solution higher than that in the culture medium (Alsharabasy et al, 2022). 37 …”

Section: Resultsmentioning

confidence: 99%

“…The effects of different concentrations of DETA-NO on the metabolic activity of MDA-MB-231 were evaluated before. 37 To assess the possibility of the remaining NO donor or H 2 O 2 in the lyophilized HA products, these were dissolved in PBS containing 1 mM luminol, and the luminescence readings were measured over time. This was to find whether these products contain certain components which can enhance the luminescence intensity, such as ROS or RNS.…”

Section: Resultsmentioning

confidence: 99%

“…It is important to note that the • NO measurements were performed in PBS, so the influence of HA on • NO levels can be investigated with minimal interference from the culture medium components on DETA-NO degradation. A comparison of • NO release from 30 and 300 μM DETA-NO in phosphate buffer and the FBS-containing medium showed a degradation rate of the NO-donor in the former solution higher than that in the culture medium (Alsharabasy et al, 2022) …”

Section: Resultsmentioning

confidence: 99%

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Interactions between Nitric Oxide and Hyaluronan Implicate the Migration of Breast Cancer Cells

et al. 2022

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Nitric oxide ( • NO) is one of the prominent free radicals, playing a pivotal role in breast cancer progression. Hyaluronic acid (HA) plays an essential role in neutralizing free radicals in tumor tissues. However, its interactions with nitric oxide have not been thoroughly investigated. Hence, this study attempts to understand the mechanism of these interactions and the different effects on the intracellular • NO levels and migration of breast cancer cells. The affinity of HA to scavenge • NO was investigated alongside the accompanying changes in specific physico-chemical properties and the further effects on the • NO-induced attachment and migration of the breast cancer cell lines, MDA-MB-231 and HCC1806. The reaction of the nitrogen dioxide radical, formed via • NO/O 2 interactions, with HA initiated a series of oxidative reactions, which, in the presence of • NO, induce the fragmentation of the polymeric chains. Furthermore, these interactions were found to hinder the NO-induced migration of cancer cells. However, the NO-induced HA modification/fragmentation was inhibited in the presence of hemin, a NO-scavenging compound. Collectively, these results help toward understanding the involvement of HA in the • NO-induced cell migration and suggest the possible modification of HA, used as one of the main materials in different biomedical applications.

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Section: Resultsmentioning

confidence: 53%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Interactions between Nitric Oxide and Hyaluronan Implicate the Migration of Breast Cancer Cells

et al. 2022

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Facile Synthesis of Hemin Derivatives with Modulated Aggregation Behaviour and Enhanced Nitric‐Oxide Scavenging Properties as New Therapeutics for Breast Cancer

Alsharabasy,

Cherukaraveedu,

Warneke

et al. 2024

Small Science

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Nitric oxide (•NO) plays various pathophysiological roles in breast cancer, significantly influencing the migration of tumour cells through concentration gradients. Therefore, modulating •NO levels via selective scavenging presents a promising approach to treating aggressive •NO‐dependent cancers, such as triple‐negative breast cancer (TNBC). Hemin emerges as a potential scavenger of •NO; however, its metalloporphyrin molecules tend to aggregate in physiological solutions, which limits its biomedical applications. To address this, a modification strategy is employed to minimize aggregation and protect against physiological oxidative degradation while preserving •NO‐scavenging properties. This is achieved through a simple chemical transformation that involves hemin conjugation to aromatic residues, tyrosine, and tyramine via carbodiimide reactions. These derivatives exhibit altered electronic properties and oxidation potential compared to hemin, alongside reduced aggregation tendencies and retained •NO‐binding affinity in aqueous solutions. Furthermore, depending on the type of hemin derivative, there is an associated inhibition of TNBC cell migration. These model hemin compounds demonstrate varying •NO‐binding affinities and resistance levels to oxidative degradation and aggregation, offering insights into the design of •NO‐scavenging molecules with enhanced properties for cancer treatment.

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Heme and Cu2+-induced vasoactive intestinal peptide (VIP) tyrosine nitration: A possible molecular mechanism for the attenuated anti-inflammatory effect of VIP in inflammatory diseases

Zeng,

Zhang,

Xia

et al. 2023

Biochimie

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Protein nitration induced by Hemin/NO: A complementary mechanism through the catalytic functions of hemin and NO-scavenging

Cited by 13 publications

References 87 publications

Interactions between Nitric Oxide and Hyaluronan Implicate the Migration of Breast Cancer Cells

Interactions between Nitric Oxide and Hyaluronan Implicate the Migration of Breast Cancer Cells

Facile Synthesis of Hemin Derivatives with Modulated Aggregation Behaviour and Enhanced Nitric‐Oxide Scavenging Properties as New Therapeutics for Breast Cancer

Heme and Cu2+-induced vasoactive intestinal peptide (VIP) tyrosine nitration: A possible molecular mechanism for the attenuated anti-inflammatory effect of VIP in inflammatory diseases

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