Protein–nanoparticle interactions evaluation by immunomethods: Surfactants can disturb quantitative determinations

Fornaguera, Cristina; Calderó, Gabriela; Solans, Conxita; Vauthier, Christine

doi:10.1016/j.ejpb.2015.05.025

Cited by 7 publications

(2 citation statements)

References 23 publications

(48 reference statements)

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“…These size properties make them adequate for an intravenous administration. [31,32] After freeze-drying, surface charge and encapsulation efficiency were also measured to ensure that they were appropriate for intravenous administration. Surface charges were positive, as expected, due to the presence of cationic oligopeptides at the end termini of the polymers ( Figure 1A,C).…”

Section: Physicochemical Characterization Of the Complexesmentioning

confidence: 99%

mRNA Delivery System for Targeting Antigen‐Presenting Cells In Vivo

Fornaguera

Guerra-Rebollo

Lázaro

et al. 2018

Adv Healthcare Materials

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110

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The encapsulation of mRNA in nanosystems as gene vaccines for immunotherapy purposes has experienced an exponential increase in recent years. Despite the many advantages envisaged within these approaches, their application in clinical treatments is still limited due to safety issues. These issues can be attributed, in part, to liver accumulation of most of the designed nanosystems and to the inability to transfect immune cells after an intravenous administration. In this context, this study takes advantage of the known versatile properties of the oligopeptide end-modified poly (β-amino esters) (OM-PBAEs) to complex mRNA and form discrete nanoparticles. Importantly, it is demonstrated that the selection of the appropriate end-oligopeptide modifications enables the specific targeting and major transfection of antigen-presenting cells (APC) in vivo, after intravenous administration, thus enabling their use for immunotherapy strategies. Therefore, with this study, it can be confirmed that OM-PBAE are appropriate systems for the design of mRNA-based immunotherapy approaches aimed to in vivo transfect APCs and trigger immune responses to fight either tumors or infectious diseases.

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Section: Physicochemical Characterization Of the Complexesmentioning

confidence: 99%

mRNA Delivery System for Targeting Antigen‐Presenting Cells In Vivo

Fornaguera

Guerra-Rebollo

Lázaro

et al. 2018

Adv Healthcare Materials

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110

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“…For radial immunodiffusion, the calibration curve consists in a linear relationship between the square of the diameter of the sedimentation rings and the concentration of the protein in the well; for rocket immunoelectrophoresis, it is a linear relationship between the height of the immunoprecipitation peak and the protein concentration in the well [ 10 , 30 ]. Another important drawback is the difficulty of performing these techniques with surfactant-containing samples, since some surfactants, such as the widely used polysorbate 80, have been described to perturb the quantification of the results [ 38 ].…”

Section: Experimental Techniques For the Analysis Of Nanoparticle mentioning

confidence: 99%

Methods for the In Vitro Characterization of Nanomedicines—Biological Component Interaction

Fornaguera

Solans

2017

JPM

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The design of colloidal nanosystems intended for biomedical applications, specifically in the field of personalized medicine, has increased notably in the last years. Consequently, a variety of characterization techniques devoted to studying nanomedicine interactions with proteins and cells have been developed, since a deep characterization of nanosystems is required before starting preclinical and clinical studies. In this context, this review aims to summarize the main techniques used to assess the interaction of nanomedicines with biological systems, highlighting their advantages and disadvantages. Testing designed nanomaterials with these techniques is required in order to have more information about their behavior on a physiological environment. Moreover, techniques used to study the interaction of nanomedicines with proteins, such as albumin and fibrinogen, are summarized. These interactions are not desired, since they usually are the first signal to the body for the activation of the immune system, which leads to the clearance of the exogenous components. On the other hand, techniques for studying the cell toxicity of nanosystems are also summarized, since this information is required before starting preclinical steps. The translation of knowledge from novel designed nanosystems at a research laboratory scale to real human therapies is usually a limiting or even a final point due to the lack of systematic studies regarding these two aspects: nanoparticle interaction with biological components and nanoparticle cytotoxicity. In conclusion, this review will be a useful support for those scientists aiming to develop nanosystems for nanomedicine purposes.

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