Protein–Nanoparticle Complex Structure Determination by Cryo-Electron Microscopy

Sen, Sagnik; Thaker, Amar; Sirajudeen, Luqmanal; Williams, Dewight; Nannenga, Brent L.

doi:10.1021/acsabm.2c00130

Cited by 3 publications

(3 citation statements)

References 40 publications

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“…It was because that soy protein is prone to flocculation and often exists in the form of flocculated particles. As soy proteins are sensitive to the surrounding elements (e.g., temperature, pH, and ionic strength), there are some discrepancies between the above particle size data and those in some references …”

Section: Resultsmentioning

confidence: 81%

“…As soy proteins are sensitive to the surrounding elements (e.g., temperature, pH, and ionic strength), there are some discrepancies between the above particle size data and those in some references. 48 The type of Pickering emulsion was decided by the wettability (expressed by the contact angle θ) of the particles. 49 The contact angle is related to many factors.…”

Section: ■ Introductionmentioning

confidence: 99%

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Soy Protein Isolate/Genipin-Based Nanoparticles for the Stabilization of Pickering Emulsion to Design Self-Healing Guar Gum-Based Hydrogels

et al. 2023

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Nowadays, stretchable self-healing hydrogels designed by biomass-based materials have gathered remarkable attention in numerous frontier fields such as wound healing, health monitoring issues, and electronic skin. In this study, soy protein isolate (SPI), a common plant protein, was cross-linked to nanoparticles (SPI NPs) by Genipin, (Gen) which was attracted from the native Geniposide. Oil-in-water (O/W) Pickering emulsion was formed by SPI NPs wrapping the linseed oil, and further implanted into poly(acrylic acid)/guar gum (PAA/GG)-based self-healing hydrogels by multiple reversible weak interactions. With the addition of Pickering emulsion, the hydrogels have achieved a remarkable self-healing ability (self-healing efficiency could reach 91.6% within 10 h) and mechanical properties (tensile strength of 0.89 MPa and strain of 853.2%). Therefore, these hydrogels with good reliable durability have outstanding application prospects in sustainable materials.

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Section: Resultsmentioning

confidence: 81%

Section: ■ Introductionmentioning

confidence: 99%

Soy Protein Isolate/Genipin-Based Nanoparticles for the Stabilization of Pickering Emulsion to Design Self-Healing Guar Gum-Based Hydrogels

et al. 2023

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“…Over time, these proteins may disassociate, making room for others with lower concentrations, slower exchange rates or stronger affinities. This phenomenon, encompassing the competitive adherence of proteins to a finite surface based on abundance, affinities and incubation time, is commonly referred to as the “Vroman Effect” [ 52 , 53 ]. This effect holds considerable significance concerning the distribution of particles within the body.…”

Section: Functionalization Of Au Npsmentioning

confidence: 99%

Review of Advances in Coating and Functionalization of Gold Nanoparticles: From Theory to Biomedical Application

Arcos Rosero,

Bueno Barbezan,

Daruich de Souza

et al. 2024

Pharmaceutics

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Nanoparticles, especially gold nanoparticles (Au NPs) have gained increasing interest in biomedical applications. Used for disease prevention, diagnosis and therapies, its significant advantages in therapeutic efficacy and safety have been the main target of interest. Its application in immune system prevention, stability in physiological environments and cell membranes, low toxicity and optimal bioperformances are critical to the success of engineered nanomaterials. Its unique optical properties are great attractors. Recently, several physical and chemical methods for coating these NPs have been widely used. Biomolecules such as DNA, RNA, peptides, antibodies, proteins, carbohydrates and biopolymers, among others, have been widely used in coatings of Au NPs for various biomedical applications, thus increasing their biocompatibility while maintaining their biological functions. This review mainly presents a general and representative view of the different types of coatings and Au NP functionalization using various biomolecules, strategies and functionalization mechanisms.

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Characterizing protein–surface and protein–nanoparticle conjugates: Activity, binding, and structure

Correira

Handali

Webb

2022

The Journal of Chemical Physics

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Many sensors and catalysts composed of proteins immobilized on inorganic materials have been reported over the past few decades. Despite some examples of functional protein-surface and protein-nanoparticle conjugates, thorough characterization of the biological-abiological interface at the heart of these materials and devices is often overlooked in lieu of demonstrating acceptable system performance. This has resulted in a focus on generating functioning protein-based devices without a concerted effort to develop reliable tools necessary to measure the fundamental properties of the bio-abio interface such as surface concentration, biomolecular structure, and activity. In this Perspective we discuss current methods used to characterize these critical properties of devices that operate by integrating a protein into both flat surfaces and nanoparticle materials. We highlight the advantages and drawbacks of each method as they relate to understanding the function of the protein-surface interface, and explore the manner in which an informed understanding of this complex interaction leads directly to the advancement of protein-based materials and technology.

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Protein–Nanoparticle Complex Structure Determination by Cryo-Electron Microscopy

Cited by 3 publications

References 40 publications

Soy Protein Isolate/Genipin-Based Nanoparticles for the Stabilization of Pickering Emulsion to Design Self-Healing Guar Gum-Based Hydrogels

Soy Protein Isolate/Genipin-Based Nanoparticles for the Stabilization of Pickering Emulsion to Design Self-Healing Guar Gum-Based Hydrogels

Review of Advances in Coating and Functionalization of Gold Nanoparticles: From Theory to Biomedical Application

Characterizing protein–surface and protein–nanoparticle conjugates: Activity, binding, and structure

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