Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells

Abstract

Protein-coated resorbable synthetic polymeric nanofibrous membranes are promising for the fabrication of advanced skin substitutes. We fabricated electrospun polylactic acid and poly(lactide- co -glycolic acid) nanofibrous membranes and coated them with fibrin or collagen I. Fibronectin was attached to a fibrin or collagen nanocoating, in order further to enhance the cell adhesion and spreading. Fibrin regularly formed a coating around individual nanofibers in the membranes, and also formed a thin noncontinuous nanofibrous mesh on top of the membranes. Collagen also coated most of the fibers of the membrane and randomly created a soft gel on the membrane surface. Fibronectin predominantly adsorbed onto a thin fibrin mesh or a collagen gel, and formed a thin nanofibrous structure. Fibrin nanocoating greatly improved the attachment, spreading, and proliferation of human dermal fibroblasts, whereas collagen nanocoating had a positive influence on the behavior of human HaCaT keratinocytes. In addition, fibrin stimulated the fibroblasts to synthesize fibronectin and to deposit it as an extracellular matrix. Fibrin coating also showed a tendency to improve the ultimate tensile strength of the nanofibrous membranes. Fibronectin attached to fibrin or to a collagen coating further enhanced the adhesion, spreading, and proliferation of both cell types.

Morphology of a fibrin nanocoating influences dermal fibroblast behavior

“…The wet pristine membrane and the membrane with an F1 or F2 nanocoating were tested using the puncture testing protocol, described in detail in our earlier study. 13 Six samples were used for each experimental group. In brief, the samples were placed in a customized holder and were punctured with a spherical cup probe at a constant speed of 0.3 mm/s (stepper motor PD28-3-1021; Trinamic, Hamburg, Germany) while the force was being recorded (1,000 Hz, Force sensor Kistler 9203; Kistler, Winterthur, Switzerland).…”

“…Moreover, the coated nanofibrous membrane showed good mechanical properties for withstanding the traction forces generated by fibroblasts. 12,13 In this study, we prepared electrospun PLA membranes with fibrin nanocoatings of various structures. The first nanocoating only coated each individual membrane fiber.…”

“…In addition, we attached Fn to the fibrin nanocoating to enhance cell adhesion. 13 The purpose of this study was to compare the behavior of human dermal fibroblasts on fibrin-coated membranes depending on the different structure of the fibrin nanocoating. We observed the fibroblast adhesion, spreading, proliferation, and synthesis of ECM proteins -collagen I and Fn.…”

“…12 In a subsequent study, we observed that fibrin increased the synthesis of another extracellular protein, Fn. 13 In recent times, nanostructured materials have been applied increasingly in the fabrication of tissue substitutes. In our studies, we also use biodegradable nanofibrous membranes for cell cultivation, because these materials can provide more support for cell adhesion, proliferation, and differentiation than flat and microstructured surfaces.…”

Colon-specific pulsatile drug release provided by electrospun shellac nanocoating on hydrophilic amorphous composites

“…[15][16][17][18][19] In particular, core-shell nanostructures (with extended nanocoating and hollow nanotube) have found a major application in a wide variety of fields. 20 Numerous publications have demonstrated that they are popular for tailoring compositions and space distributions within nanoproducts. 21,22 Core-shell nanostructures should have great potential to develop materials for colon-specific (or colon-targeted) pulsatile drug-release profiles.…”