Seasonal changes in N, P, K, Ca, Mg, Fe, B, Cu, Zn, Mn, Na, and Al of first- and second-year ovulate strobili and developing seeds of Pinus resinosa Ait. were investigated. During the first year, the major macronutrients moved steadily into developing strobili. Nitrogen and P accumulated in high amounts and Ca and Mg in low amounts. Micronutrients also moved into strobili throughout the first year as did Na and Al.The seasonal pattern of uptake and accumulation of nutrients during the second year of strobilus development differed from the first-year pattern. During the period of rapid growth of strobili following fertilization, macro- and micro-nutrients continued to move into cone tissue. However, movement of Mn into strobili stopped abruptly during mid-June when strobili reached maximum size. Beginning in mid-July, N, P, and K began moving out of maturing strobili. Other macro- and micro-nutrients continued to accumulate in strobili during the entire second year.At the same time that mature strobili were beginning to senesce, N, P, K, Mg, Mn, Zn, and Fe rapidly moved into developing seeds. Nitrogen particularly accumulated in high concentrations. Senescing cone tissue appeared to supply much of the N, P, and K that was translocated into seeds. Calcium was present in very low concentrations in seeds whereas Na accumulated in high amounts. Aluminum did not move into seeds after fertilization occurred.