Protein metabolism during an acute phase response in chickens

Abstract

Fractional rates of liver, muscle, plasma and acute phase portein synthesis were measured in chickens injected with saline or E. coli lipopolysaccharide (LPS). Male Single Comb White Leghorns were infused with a primed constant infusion of 15N-L-methionine and 2H5-L-phenylalanine into the portal vein for 2 h. Changes in plasma amino acid enrichment were similar for both amino acids reaching an apparent plateau by the 30 min sampling time. The enrichment of plasma protein-bound amino acid was measurable after 1 h of isotope infusion and increased linearly over 2h. LPS injection decreased free phenylalanine enrichment in the carotid artery (50%), and reduced tissue free methionine enrichment in the liver, pectoralis, and gastrocnemius by 16, 41, and 31% respectively. Isotopic enrichment of phenylalanine in liver protein, plasma protein and hemopexin increased in LPS injected birds relative to control birds. Fractional rates of muscle protein synthesis were not affected by LPS injection, however, liver protein, plasma protein, and hemopexin fractional synthesis rates increased 141, 161 and 266% respectively compared with untreated animals.

Serum acute phase response (APR)-related proteome of loach to trauma

“…In contrast to other methods [17][18][19][20], our approach displayed several APR-related proteins on 2-D gels and it was possible to identify these spots quickly by MALDI-TOF/MS and ESI-MS/MS. The approach demonstrates that comparative proteomics can greatly improve the chances of APP identification.…”

Net effect of an acute phase response—Partial alleviation with probiotic supplementation

“…The characterization of APR in chicken after an LPS challenge has been well defined by the manifestations of inflammation, fever, altered cytokine profile and muscle catabolism, and accompanied anorexia (Klasing, 1998b(Klasing, , 2007Barnes et al, 2002;Gruys et al, 2005;Millet et al, 2007). In regards to animal nutrition, with its emphasis on nutrient efficiencies, the characteristics of which performance-associated parameters (e.g., body tissue loss and anorexia) are affected have received considerable attention for their economic importance in poultry production.…”

Acute phase proteins: a review of their function, behaviour and measurement in chickens

“…Its predominant function is to sequester and transport haem, though it is a multifunctional protein having involvement in iron homeostasis, antioxidant production and signalling pathways that promote cell survival and gene expression (Tolosano et al, 2010). Barnes et al (2002), using E. coli LPS to induce an APR found a 2.6 fold increase in HX. Adler et al (2001) used S. typhimurium LPS and complete Freund's adjuvant and found HX levels to increase three fold at 24 hours post infection, HX levels were still significantly higher than the control birds 14 days after the LPS challenge.…”