Previous studies (1-6) of experimental ascites primarily concerned with protein metabolism and exchange have emphasized the importance of sodium in ascitic fluid production. Recently, the importance of sodium retention in several forms of effusions and edema has been described by numerous observers (7-15) who have all directly or indirectly implicated the kidney in the causal relationships of this abnormality of sodium' metabolism. This report concerns sodium, potassium, and chloride balance studies in dogs with experimentally produced ascites. Following the administration of large amounts of sodium, these ascitic animals, in contrast to the normal dog, do not excrete the excess sodium in their urine. Whereas normally in the dog there is a slightly delayed excretion of sodium with temporary retention of sodium in extracellular and intracellular spaces, these ascitic animals produce predictable amounts of ascites following an increased salt load. Potassium is promptly excreted by the kidneys and chloride is retained only when there is sodium retention. The ascites of these animals seems to resemble in some ways that of the human cirrhotic and the patient with right sided heart failure or chronic constrictive pericarditis. The liver seems implicated in this abnormality of sodium metabolism.
EXPERIMENTAL METHODSHealthy mongrel dogs averaging 8 to 12 kilograms in weight were selected for these experiments following observation for several weeks under standard conditions in the animal house. Aluminum bands were used for partial and complete occlusion of the portal vein and the vena cava below and above the liver by techniques previously described (2). Two animals were studied before and after partial and complete occlusion of the portal vein and the vena cava below the liver and above the kidneys. A third animal was studied before and after three operative procedures that first partially, then completely, occluded the portal vein and the vena cava below the liver and above the kidneys, and finally partially occluded the vena cava above the liver. The fourth and fifth animals were studied following partial occlusion of the inferior vena cava above the liver.During these periods of observation the animals were maintained in metabolism cages with a constant daily diet of horsemeat, 200 or 250 grams, and 50 grams of a low protein mixture (16) that contained sodium, potassium, chloride, and nitrogen in amounts indicated (Table I). The daily dietary intake, therefore, was 14.5 milliequivalents of potassium, 8.5 milliequivalents of sodium, and 5.6 milliequivalents of chloride for animal 50-5, and was 17.0 milliequivalents of potassium, 9.0 milliequivalents of sodium, and 6.4 milliequivalents of chloride for the other experimental animals. Additional sodium chloride and potassium (neocurtasal) 1 were given orally in capsules in large amounts at appropriate periods indicated in the accompanying graphs. Water was allowed ad libitum. During these periods complete balance studies of sodium, potassium, chloride, water, and protei...