Protein-mediated synthesis, pH-induced reversible agglomeration, toxicity and cellular interaction of silver nanoparticles

Ashraf, Sumaira; Abbasi, Azhar Z.; Pfeiffer, Christian; Hussain, Syed Zajif; Khalid, Zafar M.; Rivera-Gil, Pilar; Parak, Wolfgang J.; Hussain, Irshad

doi:10.1016/j.colsurfb.2012.09.032

Cited by 98 publications

(48 citation statements)

References 61 publications

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“…Addition of NaOH ensures that the amino acids of casein are deprotonated, providing the mono anion form in an aqueous solution making it an stronger complexing agent (Ghodake et al 2010). Ashraf et al (2013) explained that the tyrosine and histidine residues of casein may be responsible for the reduction of ionic silver in silver nanoparticle. The reduction potential of carboxyl groups increased at higher pH (Ashraf et al 2013) and so pH 11 and 13 was used to synthesis of casein-silver conjugated nanoparticle in the present investigation.…”

Section: Resultsmentioning

confidence: 99%

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Characterization of casein and casein-silver conjugated nanoparticle containing multifunctional (pectin–sodium alginate/casein) bilayer film

Bora

Mishra

2016

J Food Sci Technol

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Casein nano particles and casein-silver conjugated nano composite containing edible bilayer pouch was developed from a heat sealable casein layer laminated with sodium alginate-pectin layer. The physicochemical, mechanical, biodegradability and the toxicity of the film were evaluated. The synthesized casein nano particle was incorporated in the casein layer (inner layer) of the film, however, the casein-silver conjugated nano composite were incorporated in the sodium alginate-pectin (outer) layer of the film. The mechanical, barrier, physical and antimicrobial properties of the film were investigated. Addition of nano composite/nano particle reduced the water solubility (from 67 to 46 % at 80°C temperature) of the film improved the water barrier properties, light barrier properties, tensile strength and thermal properties of the film. The light barrier properties of the film increased and transparency reduced with increase in silver content in the conjugated nano composite film. The nano particles/nano composite containing film showed antibacterial activity against E. coli. The films were recorded safe for red blood cells as % haemolysis for all the tested samples were found to be well below the safe level.

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Section: Resultsmentioning

confidence: 99%

“…Casein-silver nano composite was prepared as per the method of Ashraf et al (2013). 50 mL of a warm suspension (at 37°C) of casein (1 %) in tris-buffer was prepared and the pH of the casein solution was adjusted to a predetermined level by using 5 M NaOH.…”

Section: Synthesis Of Casein-silver Conjugated Nano Particlesmentioning

confidence: 99%

Characterization of casein and casein-silver conjugated nanoparticle containing multifunctional (pectin–sodium alginate/casein) bilayer film

Bora

Mishra

2016

J Food Sci Technol

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“…Interestingly, they also observed that citrate coating may undergo chemical changes during drying process rendering them more cytotoxic, genotoxic and phototoxic (115). Protein ligations on the surface of AgNPs enhanced their cellular biocompatibility and kept them dispersed in cytoplasm of fibroblast cells (119). Yang et al determined toxicity of AgNPs with different ligands in order of Gum Arabic>PVP>citrate (28).…”

Section: Surface Chemistrymentioning

confidence: 99%

Nanotoxicity of Inert Materials: The Case of Gold, Silver and Iron

et al. 2016

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-Nanotechnology has opened a new horizon of research in various fields including applied physics, chemistry, electronics, optics, robotics, biotechnology and medicine. In the biomedical field, nanomaterials have shown remarkable potential as theranostic agents. Materials which are considered inert are often used in nanomedicine owning to their nontoxic profile. At nanoscale, these inert materials have shown unique properties that differ from bulk and dissolved counterparts. In the case of metals, this unique behavior not only imparts paramount advantages but also confers toxicity due to their unwanted interaction with different cellular processes. In the literature, the toxicity of nanoparticles made from inert materials has been investigated and many of these have revealed toxic potential under specific conditions. The surge to understand underlying mechanism of toxicity has increased and different means have been employed to overcome toxicity problems associated with these agents. In this review, we have focused nanoparticles of three inert metallic materials i.e. gold, silver and iron as these are regarded as biologically inert in the bulk and dissolved form. These materials have gained wider research interest and studies indicating the toxicity of these materials are also emerging. Oxidative stress, physical binding and interference with intracellular signaling are the major role player in nanotoxicity and their predominance is highly dependent upon size, surface coating and administered dose of nanoparticles. Current strategies to overcome toxicity have also been reviewed in the light of recent literature. The authors also suggested that uniform testing standards and well-designed studies are needed to evaluate nanotoxicity of these materials that are otherwise considered as inert.

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“…However, this conjugation method proved to be effective when used in the development of immunoassays employing metal nanoparticles, 13 and no aggregation was observed before addition of NaClO4. [42][43][44] Additionally, the effect of anti-hIgG concentration was investigated in the range of 150 and 750 ng mL -1 . The best performance was observed using 150 ng mL -1 anti-hIgG.…”

Section: Resultsmentioning

confidence: 99%

“…43 This pattern was also reported in other systems in which the pH was near the isoelectric point of the protein attached to the metal NP. 44 Moreover, the charge distribution of the biomolecules involved is a factor of great influence in the stabilization process. The anomalous stability of latex colloidal particles conjugated to immunoglobulins with increasing ionic strength is attributed to hydration forces.…”

Section: Resultsmentioning

confidence: 99%

Immunoassay for Human IgG Using Antibody-functionalized Silver Nanoparticles

2017

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A simple colorimetric immunoassay for quantification of human immunoglobulin G (hIgG) is herein described. The assay is based on the aggregation inhibition of silver nanoparticles (AgNP) functionalized with hIgG antibody (anti-hIgG) on the surface. The aggregation is measured in terms of attenuance values ratio at 400 and 530 nm (A400/A530). A linear response between A400/A530 and hIgG concentration is observed in the range 25 -200 ng mL -1 , and the detection limit is estimated as 11 ng mL -1 hIgG.

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Protein-mediated synthesis, pH-induced reversible agglomeration, toxicity and cellular interaction of silver nanoparticles

Cited by 98 publications

References 61 publications

Characterization of casein and casein-silver conjugated nanoparticle containing multifunctional (pectin–sodium alginate/casein) bilayer film

Characterization of casein and casein-silver conjugated nanoparticle containing multifunctional (pectin–sodium alginate/casein) bilayer film

Nanotoxicity of Inert Materials: The Case of Gold, Silver and Iron

Immunoassay for Human IgG Using Antibody-functionalized Silver Nanoparticles

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