Protein mass spectrometry detects multiple bloodmeals for enhanced Chagas disease vector ecology

Keller, Judith I.; Lima-Cordón, Raquel Asunción; Monroy, María Carlota; Schmoker, Anna M.; Zhang, Fan; Howard, Ann; Ballif, Bryan A.; Stevens, Lori

doi:10.1016/j.meegid.2019.103998

Cited by 4 publications

(15 citation statements)

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“…To cite just a few examples, proteomics has been used to characterize a victim's stomach contents upon autopsy, 60 identify trace evidence at a crime scene as vomit, 61 identify 3,500-year-old organic residue from tombs in northwestern China as a fermented dairy product, 62 and determine the host animal that was the source of a disease-vector tick's last blood meal. 63 All of the studies just mentioned used either database search or sequence-tag search to identify proteins. The first three all used a very large database that includes many species, such as UniProt (around 100 million sequences) or NCBI (around 20 million sequences in this case, representing a considerable down-selection of the 800 million currently listed).…”

Section: Forensics and Related Areasmentioning

confidence: 99%

“…In addition to traditional (criminal justice) forensics, other fields also face the problem of characterizing a sample when little or nothing is known about its identity or origin, such as archaeology and cultural heritage. To cite just a few examples, proteomics has been used to characterize a victim's stomach contents upon autopsy, 60 identify trace evidence at a crime scene as vomit, 61 identify 3,500‐year‐old organic residue from tombs in northwestern China as a fermented dairy product, 62 and determine the host animal that was the source of a disease‐vector tick's last blood meal 63 …”

Section: Application Areasmentioning

confidence: 99%

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Flying blind, or just flying under the radar? The underappreciated power of de novo methods of mass spectrometric peptide identification

2020

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Mass spectrometry‐based proteomics is a popular and powerful method for precise and highly multiplexed protein identification. The most common method of analyzing untargeted proteomics data is called database searching, where the database is simply a collection of protein sequences from the target organism, derived from genome sequencing. Experimental peptide tandem mass spectra are compared to simplified models of theoretical spectra calculated from the translated genomic sequences. However, in several interesting application areas, such as forensics, archaeology, venomics, and others, a genome sequence may not be available, or the correct genome sequence to use is not known. In these cases, de novo peptide identification can play an important role. De novo methods infer peptide sequence directly from the tandem mass spectrum without reference to a sequence database, usually using graph‐based or machine learning algorithms. In this review, we provide a basic overview of de novo peptide identification methods and applications, briefly covering de novo algorithms and tools, and focusing in more depth on recent applications from venomics, metaproteomics, forensics, and characterization of antibody drugs.

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Section: Forensics and Related Areasmentioning

confidence: 99%

Section: Application Areasmentioning

confidence: 99%

Flying blind, or just flying under the radar? The underappreciated power of de novo methods of mass spectrometric peptide identification

2020

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“…In particular, understanding vector peridomiciliary and intradomiciliary movement, colonization and blood meal sources are important for the success of vector control programs [ 8 , 12 , 13 ]. Various methods have been used to identify blood meal sources, including the Polymerase Chain Reaction (PCR) to target remnants of vertebrate DNA from traces of blood in the insect abdomen [ 8 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…Various methods have been used to identify blood meal sources, including the Polymerase Chain Reaction (PCR) to target remnants of vertebrate DNA from traces of blood in the insect abdomen [ 8 , 12 ]. However, the detection ability of DNA decreases rapidly after feeding [ 13 ] and for some communities where blood meal sources may be scarce, over 60% of peridomicile and intradomicile collected bugs had no blood source detected using DNA based methods [ 8 , 11 ]. A new method for blood meal detection in Chagas vectors is now available, using liquid chromatography tandem mass spectrometry (LC-MS/MS) based on variation in vertebrate hemoglobin peptides.…”

Section: Introductionmentioning

confidence: 99%

“…A new method for blood meal detection in Chagas vectors is now available, using liquid chromatography tandem mass spectrometry (LC-MS/MS) based on variation in vertebrate hemoglobin peptides. In a previous study, protein-based LC-MS/MS detected at least one blood source in 100% of the samples, and an average of 1.6 blood sources per bug, compared to 50% and 0.5 using DNA; however, the bioinformatic pipeline was not designed to analyze a large number of samples [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Assessing risk of vector transmission of Chagas disease through blood source analysis using LC-MS/MS for hemoglobin sequence identification

et al. 2022

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Chagas disease is mainly transmitted by triatomine insect vectors that feed on vertebrate blood. The disease has complex domiciliary infestation patterns and parasite transmission dynamics, influenced by biological, ecological, and socioeconomic factors. In this context, feeding patterns have been used to understand vector movement and transmission risk. Recently, a new technique using Liquid chromatography tandem mass spectrometry (LC-MS/MS) targeting hemoglobin peptides has showed excellent results for understanding triatomines’ feeding patterns. The aim of this study was to further develop the automated computational analysis pipeline for peptide sequence taxonomic identification, enhancing the ability to analyze large datasets data. We then used the enhanced pipeline to evaluate the feeding patterns of Triatoma dimidiata, along with domiciliary infestation risk variables, such as unkempt piles of firewood or construction material, cracks in bajareque and adobe walls and intradomiciliary animals. Our new python scripts were able to detect blood meal sources in 100% of the bugs analyzed and identified nine different species of blood meal sources. Human, chicken, and dog were the main blood sources found in 78.7%, 50.4% and 44.8% of the bugs, respectively. In addition, 14% of the bugs feeding on chicken and 15% of those feeding on dogs were captured in houses with no evidence of those animals being present. This suggests a high mobility among ecotopes and houses. Two of the three main blood sources, dog and chicken, were significantly (p < 0.05) affected by domiciliary infestation risk variables, including cracks in walls, construction material and birds sleeping in the intradomicile. This suggests that these variables are important for maintaining reproducing Triatoma dimidiata populations and that it is critical to mitigate these variables in all the houses of a village for effective control of these mobile vectors.

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Chagas Disease Ecology in the United States: Recent Advances in Understanding Trypanosoma cruzi Transmission Among Triatomines, Wildlife, and Domestic Animals and a Quantitative Synthesis of Vector–Host Interactions

Busselman

Hamer

2022

Annu. Rev. Anim. Biosci.

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Chagas disease, a neglected tropical disease present in the Americas, is caused by the parasite Trypanosoma cruzi and is transmitted by triatomine kissing bug vectors. Hundreds of vertebrate host species are involved in the ecology of Chagas disease. The sylvatic nature of most triatomines found in the United States accounts for high levels of animal infections but few reports of human infections. This review focuses on triatomine distributions and animal infections in the southern United States. A quantitative synthesis of available US data from triatomine bloodmeal analysis studies shows that dogs, humans, and rodents are key taxa for feeding triatomines. Imperfect and unvalidated diagnostic tools in wildlife complicate the study of animal T. cruzi infections, and integrated vector management approaches are needed to reduce parasite transmission in nature. The diversity of animal species involved in Chagas disease ecology underscores the importance of a One Health approach for disease research and management. Expected final online publication date for the Annual Review of Animal Biosciences, Volume 10 is February 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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Protein mass spectrometry detects multiple bloodmeals for enhanced Chagas disease vector ecology

Cited by 4 publications

References 40 publications

Flying blind, or just flying under the radar? The underappreciated power of de novo methods of mass spectrometric peptide identification

Flying blind, or just flying under the radar? The underappreciated power of de novo methods of mass spectrometric peptide identification

Assessing risk of vector transmission of Chagas disease through blood source analysis using LC-MS/MS for hemoglobin sequence identification

Chagas Disease Ecology in the United States: Recent Advances in Understanding Trypanosoma cruzi Transmission Among Triatomines, Wildlife, and Domestic Animals and a Quantitative Synthesis of Vector–Host Interactions

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