Protein Markers for Somatic Embryogenesis

Tchorbadjieva, M.

doi:10.1007/7089_029

Cited by 19 publications

(18 citation statements)

References 79 publications

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“…Suspension cultures provide the opportunity to study the role of extracellular molecules secreted into the culture media in the processes of embryo development as well as to identify molecular markers for somatic embryogenesis among these molecules [42].…”

Section: Discussionmentioning

confidence: 99%

The role of chitinases and glucanases in somatic embryogenesis of black pine and hybrid firs

et al. 2013

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Glucanase and chitinase enzymes play an important role in different plant processes including defense against pathogens and morphogenesis. Moreover, their role in the processes of somatic embryogenesis has been demonstrated. It has been suggested, that the presence of this type of proteins might be a marker for embryogenic potential of callus cultures. In this work we screened for the presence of glucanases and chitinases in liquid growth media of a set of conifer embryogenic cell lines in order to find correlation with their embryogenic potential. We have found that none of the 12 chitinase isoforms detected in culture media of Pinus nigra Arn. or the nine chitinases detected in media with Abies alba × A. cephalonica and Abies alba × A. numidica embryogenic tissues could be linked to their embryogenic capacity. Similarly, none of the six glucanase isoforms detected in the extracellular fluid of Pinus nigra Arn. cultures can be assigned as a marker of embryogenic potential. Thus, our data indicate the large variability and doubtless importance of glucanases and chitinases for cell growth and development of somatic embryos, however, do not support the premise that they are markers of embryogenesis.

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Section: Discussionmentioning

confidence: 99%

The role of chitinases and glucanases in somatic embryogenesis of black pine and hybrid firs

et al. 2013

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“…Studies on the role of secreted proteins and their use as markers for somatic embryogenesis in monocots are relatively scarce (Nielsen and Hansen, 1992;Stirn et al, 1995;Tchorbadjieva et al, 2005;Tchorbadjieva, 2006). In search for embryogenic markers, we identified 11/12 kDa proteins constitutively secreted in the medium of embryogenic suspension cultures as acidic nsLTP-like proteins with as yet unknown function.…”

Section: Introductionmentioning

confidence: 99%

Generation of Recombinant Antibodies against Orchardgrass Acidic nsLTP-Like Proteins

Rakleova

Tsacheva

Petkova

et al. 2008

Zeitschrift Für Naturforschung C

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Embryogenic and non-embryogenic suspension cultures of orchardgrass (Dactylis glomerata L.) secreted into the culture medium a set of proteins, among which low molecular mass (11/12 kDa) proteins were found. However, only the 11/12 kDa proteins from the embryogenic suspension cultures reacted specifically with an antiserum raised against the carrot EP2 non-specific lipid transfer protein (nsLTP). Two-dimensional (2-D) electrophoretic analysis revealed that the extracellular nsLTP-like proteins from the embryogenic lines were acidic proteins, with pI values ranging between 4.3 and 6.4, and the 11/12 kDa proteins of the nonembryogenic lines were basic ones (pI 8Ð9.3). This is only the second case to report on the accumulation of extracellular acidic nsLTP-like proteins in the culture medium during somatic embryogenesis. A naïve phage display Griffin1. library was used to select single-chain phage antibodies, which specifically bind to acidic nsLTP-like proteins. Nine phage clones were selected after four rounds of biopanning of the target proteins blotted on a nitrocellulose membrane. Three soluble monoclonal single-chain phage antibodies, expressed in the non-suppressor E. coli strain HB2151, were purified by metal affinity chromatography and found to be highly specific for the acidic nsLTP-like proteins from the embryogenic suspension cultures. The application of the selected monoclonal antibodies for localization and elucidation of the role of the acidic nsLTP-like proteins in vivo is discussed.

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“…Različiti eksperimentalni pristupi korišćeni su za izolaciju i karakterizaciju markera somatske embriogeneze. U većini slučajeva rađena je analiza ukupnih proteina iz embriogenih i neembriogenih ćelija, pa je njihovim upoređivanjem dobijena delimična slika proteina koji su uključeni samo u proces somatske embriogeneze (Tchorbadjieva, 2005). Na taj način dobija se ogroman broj različitih proteina koji se ne mogu svi nazvati markerima somatske embriogeneze (Hahne i sar., 1988; Hilbert i sar., 1992).…”

Section: Biohemijski Aspekti Somatske Embriogenezeunclassified

“…Tokom indukcije somatske embriogeneze, i tokom samog procesa, dešavaju se specifične promene u pogledu sadržaja ukupnih proteina. Određeni proteini i njihove izoforme se sintetišu i mogu se detektovati, dok neke druge nestaju (Tchorbadjieva, 2005).…”

Section: Biohemijski Aspekti Somatske Embriogenezeunclassified

“…Povećanje koncentracije AGP na samom početku procesa somatske embriogeneze primećeno je i kod šargarepe (Thompson i Knox, 1998) kao i kod cikorije (Chapman i sar., 2000). AGP iz hranljive podloge mogu stimulisati formiranje somatskih embriona (Egertsdotter i Van Arnold, 1995;Tchorbadjieva, 2005;Zagorchev i sar., 2008).…”

Section: Sadržaj Arabinogalaktanskih Proteina Tokom Indukcije Morfogeunclassified

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Physiological and biochemical aspects of regeneration of snake?s head fritillary (Fritillaria meleagris L.) in vitro

Beogradu¹,

Petrić²

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Protein Markers for Somatic Embryogenesis

Cited by 19 publications

References 79 publications

The role of chitinases and glucanases in somatic embryogenesis of black pine and hybrid firs

The role of chitinases and glucanases in somatic embryogenesis of black pine and hybrid firs

Generation of Recombinant Antibodies against Orchardgrass Acidic nsLTP-Like Proteins

Physiological and biochemical aspects of regeneration of snake?s head fritillary (Fritillaria meleagris L.) in vitro

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