Protein loading, elution, and resolution behavior in a novel device that integrates ultrafiltration and chromatographic separation

Dai, Xiaoping; Majumdar, S.; Luo, Robert G.; Sirkar, Kamalesh K.

doi:10.1002/bit.10653

Cited by 5 publications

(4 citation statements)

References 29 publications

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“…To answer this question, a model is being developed to predict the device performance with the coated length as a parameter. The model is obviously much more complicated than that developed by Dai et al (20). It involves both development of a filtration model of a module having the partially coated fibers and then a chromatographic model using such a velocity profile in the coated fiber system.…”

Section: Resultsmentioning

confidence: 95%

“…Therefore, breakthrough will not happen until the beads in the coated zone are saturated. Consequently, the extent of protein leakage into the permeate solution observed in Dai et al (16,20) should be drastically reduced. Correspondingly, the overall protein loading capacity of the shell-side bed should be increased dramatically in the new device.…”

Section: Introductionmentioning

confidence: 97%

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A New Integrated Membrane Filtration and Chromatographic Device

Sirkar

Dai

et al. 2008

Biotechnol Progress

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To improve protein separation, a novel integrated device combining membrane filtration and chromatography has been developed. The device basically consists of a hollow fiber filtration module whose shell side is filled with chromatographic resin beads. However, there is an essentially impermeable coated zone near the hollow fiber module outlet. The integrated device enjoys the advantages of both membrane filtration and chromatography; it also allows one to load the chromatographic media directly from the fermentation broth or lysate and separate the adsorbed proteins through the subsequent elution step in a cyclic process. Interfacial polymerization was carried out to coat the bottom section of the hollow fiber membrane; the rest of the hollow fiber membrane remained unaffected. Myoglobin (Mb) and R-lactalbumin (R-LA) were primarily used as model proteins in a binary mixture; binary mixtures of Mb and bovine serum albumin (BSA) were also investigated. Separation behaviors of binary protein mixtures were studied in devices having either an ultrafiltration (UF) or a microfiltration (MF) membrane. Experimental results show that the breakthrough time and the protein loading capacities were dramatically improved after introducing the impermeable coating in both UF and MF modules. For a synthetic yeast fermentation broth feed, four loading-washing-elution-reequilibration-based cyclic runs for separation of Mb and R-LA were performed in the device using a MF membrane with a coated zone without cleaning in between. The Mb and R-LA elution profiles for the four consecutive runs were almost superimposable. Due to lower transmembrane flux in this device plus the periodical washing-elution during the chromatographic separation, fouling was not a problem, unlike in conventional microfiltration.

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Section: Resultsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 97%

A New Integrated Membrane Filtration and Chromatographic Device

Sirkar

Dai

et al. 2008

Biotechnol Progress

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“…A higher superficial velocity of the fluid phases will offer higher wall shear rate on the membrane surfaces, reducing gel layer formation and improve the flux [14]. Although the preceding feature is interesting from the industrial point of view, the increase in the flow rate must be balanced against the rise in the operating cost.…”

Section: Effect Of Superficial Velocitymentioning

confidence: 96%

Mass transfer mechanisms in fixed-bed adsorption of erythromycin

Sun

Zhu

Chen

et al. 2008

Front. Chem. Eng. China

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The equilibrium and kinetic characteristics of the adsorption of erythromycin to Sepabeads SP825 were determined. The equilibrium data in a batch system was well described by a Langmuir isotherm. The separation performance was investigated in a fixed-bed system with respect to the adsorption superficial velocity, ionic strength and pH. A mathematical model was used to simulate the mass transfer mechanism, taking film mass transfer, pore diffusion and axial dispersion into account. The model predictions were consistent with the experimental data and were consequently used to determine the mass transfer coefficients.

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“…Currently, hollow-fiber membrane units are used in water separations in large volume applications; they are well established in hemodialysis and the potential in gas separation is very large. Hollow fiber membranes and chromatographic resin beads are commonly employed in a variety of bioseparation processes (58). This application is a small volume, high price opportunity, but its uniqueness and value of the end-product allows for high profit margins.…”

Section: Future Prospectsmentioning

confidence: 99%

Membranes, Hollow‐Fiber

Moch¹

2005

Kirk-Othmer Encyclopedia of Chemical Technology

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Hollow‐fiber membranes were invented in the 1960s. In the ensuing decades much research and development effort has transpired with the result that these fibers have become exceedingly important to industry, academia, and government in such fields as desalination of water, wastewater reclamation, medicine, agriculture, gas separation, and pervaporation. Commercial opportunities annually exceed several hundred million dollars. The hollow‐fiber membranes are usually the base technology within a significantly larger system.This article briefly discusses the history and theory of hollow‐fiber membrane technology. Methods of manufacture are reviewed together with processing techniques employed to enhance various aspects of performance. Hollow‐fiber membranes are composed of many different materials of construction. The generic compositions are cited and specific end use applications noted. Lastly, the work to date has identified many areas where future prospects for hollow‐fiber membranes are the brightest. A number of these future growth areas are discussed together with which industrial corporations are in the forefront of the new developments.

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Protein loading, elution, and resolution behavior in a novel device that integrates ultrafiltration and chromatographic separation

Cited by 5 publications

References 29 publications

A New Integrated Membrane Filtration and Chromatographic Device

A New Integrated Membrane Filtration and Chromatographic Device

Mass transfer mechanisms in fixed-bed adsorption of erythromycin

Membranes, Hollow‐Fiber

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