Protein-loaded PLGA microparticles engineered by flow focusing: Physicochemical characterization and protein detection by reversed-phase HPLC

Holgado, M.A.; Cózar-Bernal, M.J.; Salas, Soumya; Arias, José L.; Álvarez-Fuentes, Josefa; Fernández‐Arévalo, M.

doi:10.1016/j.ijpharm.2009.07.017

Cited by 28 publications

(14 citation statements)

References 38 publications

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“…[12][13][14] The chromatographic conditions 15,16 were determined using a column C18 (Spherisorb ® ). The limits of detection and quantification were 0.5 µg/mL and 1.25 µg/mL, respectively.…”

Section: Chromatographic Conditionsmentioning

confidence: 99%

Cannabinoid derivate-loaded PLGA nanocarriers for oral administration: formulation, characterization, and cytotoxicity studies

Martín-Banderas¹,

Álvarez-Fuentes²,

Durán‐Lobato³

et al. 2012

IJN

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CB13 (1-Naphthalenyl[4-(pentyloxy)-1-naphthalenyl]methanone)-loaded poly(lactic-co-glycolic acid) nanoparticles (NPs) were produced by nanoprecipitation and tested for their in vitro release behavior and in vitro cytotoxicity assays. The effects of several formulation parameters such as polymer type, surfactant concentration, and initial drug amount were studied. NPs had a particle size 90-300 nm in diameter. Results obtained show that the main influence on particle size was the type of polymer employed during the particle production: the greater the hydrophobicity, the smaller the particle size. In terms of encapsulation efficiency (%), high values were achieved (∼68%-90%) for all formulations prepared due to the poor solubility of CB13 in the external aqueous phase. Moreover, an inverse relationship between release rate and NP size was found. On the other hand, low molecular weight and low lactide content resulted in a less hydrophobic polymer with increased rates of water absorption, hydrolysis, and erosion. NPs showed no cytotoxicity and may be considered to be appropriate for drug-delivery purposes.

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Section: Chromatographic Conditionsmentioning

confidence: 99%

Cannabinoid derivate-loaded PLGA nanocarriers for oral administration: formulation, characterization, and cytotoxicity studies

Martín-Banderas¹,

Álvarez-Fuentes²,

Durán‐Lobato³

et al. 2012

IJN

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“…Gas flow focusing [27] and electrospray [28,29] can be used to fabricate PLGA microparticles with uniform sizes but these approaches have not been widely used to generate nanoparticles. Several solvent-based methods can be used to make polymeric nanoparticles including interfacial polymerization [30], the evaporation of emulsions [31] and nanoprecipitation [32].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of PLGA nanoparticles with a fluidic nanoprecipitation system

Xie

Smith

2010

J Nanobiotechnol

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Particle size is a key feature in determining performance of nanoparticles as drug carriers because it influences circulating half-life, cellular uptake and biodistribution. Because the size of particles has such a major impact on their performance, the uniformity of the particle population is also a significant factor. Particles comprised of the polymer poly(lactic-co-glycolic acid) (PLGA) are widely studied as therapeutic delivery vehicles because they are biodegradable and biocompatible. In fact, microparticles comprised of PLGA are already approved for drug delivery. Unfortunately, PLGA nanoparticles prepared by conventional methods usually lack uniformity. We developed a novel Fluidic NanoPrecipitation System (FNPS) to fabricate highly uniform PLGA particles. Several parameters can be fine-tuned to generate particles of various sizes.

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“…The content release from PLGA capsules depends on various parameters, including size, material composition, production technique, and applied surface modification of the capsules. Accordingly, previous studies have usually focused on the release studies of a model analyte from capsules that were obtained by individual strategies . However, a direct comparison of multiple release‐affecting parameters that are measured simultaneously is usually neglected.…”

Section: Resultsmentioning

confidence: 99%

“…The entrapment of chemical agents and their subsequent release from various carriers at the microscale and nanoscale has been widely studied over the past decades. Nowadays, (bio)degradable carriers consisting of polyurethane, poly(ε‐caprolactone), and poly( d,l ‐lactic‐ co ‐glycolic acid) (PLGA) copolymers can be produced by various techniques, including spray drying, nanoprecipitation, and solvent evaporation . Among these, the focus has especially been on capsules made of PLGA because of a long list of approved properties, including biocompatibility, that have high relevance in the field of medical treatment.…”

Section: Introductionmentioning

confidence: 99%

Multifactorial design of poly(d,l‐lactic‐co‐glycolic acid) capsules with various release properties for differently sized filling agents

Schmitz-Hertzberg

Mak

Lai

et al. 2013

J of Applied Polymer Sci

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The hydrolytic degradation and corresponding content release of capsules made of poly(D,L-lactic-co-glycolic acid) (PLGA) strongly depends on the composition and material properties of the initially applied copolymer. Consecutive or simultaneous release from capsule batches of combinable material compositions, therefore, offers high control over the bioavailability of an encapsulated drug. The keynote of this study was the creation of a superordinated database that addressed the correlation between the release kinetics of filling agents with different molecular weights from PLGA capsules of alternating composition. Fluorescein isothiocyanate (FITC)-dextran (with molecular weights of 4, 40, and 2000 kDa) was chosen as a model analyte, whereas the copolymers were taken from various 50:50 PLGA, 75:25 PLGA, and polylactide blends. With reference to recent publications, the capsule properties, such as the size, morphology, and encapsulation efficiency, were further modified during production. Hence, uniform microdisperse and polydisperse submicrometer nanocapsules were prepared by two different water-in-oil-in-water emulsification techniques, and additional effects on the size and morphology were achieved by capsule solidification in two different sodium salt buffers. The qualitative and quantitative examination of the physical capsule properties was performed by confocal laser scanning microscopy, scanning electron microscopy, and Coulter counting techniques to evaluate the capsule size distribution and the morphological appearance of the different batches. The corresponding agent release was quantified by fluorescence measurement of the FITC-dextran in the incubation media and by the direct measurement of the capsule brightness via fluorescence microscopy. In summary, the observed agent release showed a highly controllable flexibility depending on the PLGA blends, preparation methods, and molecular weight of the used filling substances.

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Protein-loaded PLGA microparticles engineered by flow focusing: Physicochemical characterization and protein detection by reversed-phase HPLC

Cited by 28 publications

References 38 publications

Cannabinoid derivate-loaded PLGA nanocarriers for oral administration: formulation, characterization, and cytotoxicity studies

Cannabinoid derivate-loaded PLGA nanocarriers for oral administration: formulation, characterization, and cytotoxicity studies

Fabrication of PLGA nanoparticles with a fluidic nanoprecipitation system

Multifactorial design of poly(d,l‐lactic‐co‐glycolic acid) capsules with various release properties for differently sized filling agents

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