Protein–Lipid Interactions Stabilize the Oligomeric State of BOR1p from Saccharomyces cerevisiae

Abstract

We perform an analysis of the three-dimensional cosmic matter density field traced by galaxies of the SDSS-III/BOSS galaxy sample. The systematic-free nature of this analysis is confirmed by two elements: the successful cross-correlation with the gravitational lensing observations derived from Planck 2018 data and the absence of bias at scales k 10 −3 − 10 −2 h Mpc −1 in the a posteriori power spectrum of recovered initial conditions. Our analysis builds upon our algorithm for Bayesian Origin Reconstruction from Galaxies (borg) and uses a physical model of cosmic structure formation to infer physically meaningful cosmic structures and their corresponding dynamics from deep galaxy observations. Our approach accounts for redshift-space distortions and light-cone effects inherent to deep observations. We also apply detailed corrections to account for known and unknown foreground contaminations, selection effects and galaxy biases. We obtain maps of residual, so far unexplained, systematic effects in the spectroscopic data of SDSS-III/BOSS. Our results show that unbiased and physically plausible models of the cosmic large scale structure can be obtained from present and next-generation galaxy surveys.

Strategies for successful isolation of a eukaryotic transporter

“…It has been suggested that this radical structural movement is facilitated by the stabilising interactions formed between two monomers and possibly involving interactions with membrane lipids [42]. In the case of the BOR homologue from S. cerevisiae, ScBOR1p, we now have evidence that the protein can function as a monomer [43], although we can't rule out that the dimer plays a role in function. It is not clear which oligomeric states is the functional form of OsBOR3, a feature that is challenging to definitively decipher even if a high-resolution structure is available.…”

Transfer of stabilising mutations between different secondary active transporter families

“…The different AtBOR1 constructs were individually transformed into the FGY217 ∆ bor1p knockout S. cerevisiae strain [23]. Transformants were inoculated in 10 mL of ‐URA media supplemented with 2% w/v glucose and incubated overnight at 30 °C, 300 r.p.m.…”

Native mass spectrometry—A valuable tool in structural biology

“…104,105 Importantly, native MS directly reveals the effects of lipid binding onto the stability and formation of protein complexes. [106][107][108] Due to several drawbacks of detergent micelles, other membrane mimetics that better resemble the lipid bilayer of biological membranes are favoured and explored. 109 However, these are usually more complex including phospholipids and scaffold proteins or polymers.…”

“…However, recent improvements enabled the analysis of cooperative binding of lipids, drugs or other ligands such as nucleotides or peptides 104,105 . Importantly, native MS directly reveals the effects of lipid binding onto the stability and formation of protein complexes 106–108 …”