Protein kinase G regulates β-synuclein in response to repeated exposure to cocaine in the rat dorsal striatum in a Ca2+-dependent manner

Yang, Jialong; Choe, Eun Sang

doi:10.1016/j.neulet.2014.08.042

Cited by 2 publications

(3 citation statements)

References 25 publications

(27 reference statements)

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“…The dorsomedial striatum (DMS) is an important brain region for the transition to addiction from initial to compulsive use Caprioli et al, 2017). Within the dorsal striatum, cocaine exposure produces a number of changes to intracellular signaling cascades, in part by modulating regulation of calcium (Yang & Choe, 2014;Kim et al, 2015;Kim et al, 2009;Schierberl et al, 2012;Shen et al, 2018). In addition, intracellular calcium signaling in the striatum modulates cocaine reward and sensitization (Barr et al, 2015;Mizuno et al, 2013;Yasui and Su, 2016).…”

Section: Introductionmentioning

confidence: 99%

Intermittent but not continuous access to cocaine produces individual variability in addiction susceptibility in rats

García¹,

Webb²,

Yager³

et al. 2020

Psychopharmacology

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Drug addiction is a chronic disease defined by a complex set of characteristics, including loss of control over drug intake and persistent drug craving, which primarily affects a small percentage of people who try drugs. Although many models have been developed to study individual aspects of drug use, there is great translational value in having an animal model that encompasses multiple aspects of the human disease, including the variation in severity observed in humans. Here, we describe an intermittent access model of cocaine self-administration that produces a subset of rats that display many of the core features of addiction, including escalation of drug intake, a bingelike pattern of drug use, robust locomotor sensitization, and high levels of drug-seeking during cue-induced reinstatement. This group is compared to rats that have the same drug history but do not develop this pattern of drug-taking and drug-seeking, as well as rats that undergo a traditional continuous access paradigm. Finally, we observe that high levels of cocaine consumption produce long-term changes in intracellular calcium signaling in the dorsomedial striatum.Terms of use and reuse: academic research for non-commercial purposes, see here for full terms. http://www.springer.com/gb/openaccess/authors-rights/aam-terms-v1

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Section: Introductionmentioning

confidence: 99%

Intermittent but not continuous access to cocaine produces individual variability in addiction susceptibility in rats

García¹,

Webb²,

Yager³

et al. 2020

Psychopharmacology

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“…Since repeated cocaine exposure increases Ca 2+ influx via stimulation of glutamate receptors ( Ryu and Choe, 2014 ; Yang and Choe, 2014 ), we determined the contribution of Ca 2+ influx to the phosphorylation of DARPP32-Thr75. Intracaudate infusion of the Ca 2+ chelator, BAPTA-AM (50 nmol), significantly decreased the elevation of pDARPP32-Thr75 immunoreactivity after repeated cocaine administration ( Figure 2B ).…”

Section: Resultsmentioning

confidence: 99%

“…Cocaine was injected in a volume of 1mL in physiological saline (0.9% sodium chloride). The concentrations of drugs were determined from previous studies ( Ahn and Choe, 2009 , 2010 ; Oh et al, 2013 ; Yang and Choe, 2014 ) except for KN93 ( Anderson et al, 2008 ).…”

Section: Methodsmentioning

confidence: 99%

Activation of Protein Kinases and Phosphatases Coupled to Glutamate Receptors Regulates the Phosphorylation State of DARPP32 at Threonine 75 After Repeated Exposure to Cocaine in the Rat Dorsal Striatum in a Ca²⁺-Dependent Manner

Kim

Ryu

Seo

et al. 2015

IJNPPY

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Background:Phosphorylation state of dopamine- and cAMP-regulated phosphoprotein, molecular weight 32kDa (DARPP32) is crucial to understand drug-mediated synaptic plasticity. In this study, mechanisms underlying repeated cocaine-stimulated phosphorylation of DARPP32 at threonine 75 (pDARPP32-Thr75) were determined by investigating the hypothesis that activation of protein kinases and phosphatases coupled to glutamate signaling is necessary for the regulation of pDARPP32-Thr75 after repeated cocaine administration.Methods:Intracaudate drug infusions into the rat dorsal striatum followed by Western immunoblot analysis were mainly performed to test this hypothesis.Results:The results demonstrated that 7 repeated daily intraperitoneal injections of cocaine (20mg/kg) upregulated the expression of pDARPP32-Thr75. Increases in the cytosolic Ca2+ concentrations followed by Ca2+-dependent protein kinase activation through stimulation of Ca2+ channels in striatal neurons were necessary for the phosphorylation. Activation of protein phosphatases further regulated the phosphorylation state by deactivating pDARPP32-Thr75 and upstream protein kinases.Conclusion:These findings suggest that activation of protein kinases and phosphatases coupled to glutamate receptors controls the phosphorylation state of DARPP32-Thr75 after repeated exposure to cocaine in the dorsal striatum in a Ca2+-dependent manner.

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Protein kinase G regulates β-synuclein in response to repeated exposure to cocaine in the rat dorsal striatum in a Ca2+-dependent manner

Cited by 2 publications

References 25 publications

Intermittent but not continuous access to cocaine produces individual variability in addiction susceptibility in rats

Intermittent but not continuous access to cocaine produces individual variability in addiction susceptibility in rats

Activation of Protein Kinases and Phosphatases Coupled to Glutamate Receptors Regulates the Phosphorylation State of DARPP32 at Threonine 75 After Repeated Exposure to Cocaine in the Rat Dorsal Striatum in a Ca²⁺-Dependent Manner

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Protein kinase G regulates β-synuclein in response to repeated exposure to cocaine in the rat dorsal striatum in a Ca2+-dependent manner

Cited by 2 publications

References 25 publications

Intermittent but not continuous access to cocaine produces individual variability in addiction susceptibility in rats

Intermittent but not continuous access to cocaine produces individual variability in addiction susceptibility in rats

Activation of Protein Kinases and Phosphatases Coupled to Glutamate Receptors Regulates the Phosphorylation State of DARPP32 at Threonine 75 After Repeated Exposure to Cocaine in the Rat Dorsal Striatum in a Ca2+-Dependent Manner

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Activation of Protein Kinases and Phosphatases Coupled to Glutamate Receptors Regulates the Phosphorylation State of DARPP32 at Threonine 75 After Repeated Exposure to Cocaine in the Rat Dorsal Striatum in a Ca²⁺-Dependent Manner