Protein kinase CK2 is implicated in early steps of the differentiation of pre-adipocytes into adipocytes

Wilhelm, Nadine; Kostelnik, Katja B.; Götz, Claudia; Montenarh, Mathias

doi:10.1007/s11010-012-1241-y

Cited by 27 publications

(21 citation statements)

References 31 publications

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“…In another study, which analysed the differentiation of preadipocytes into adipocyctes it was shown that the CK2 kinase activity increased at the beginning of the differentiation process and decreased with ongoing differentiation. Moreover, inhibition of the CK2 kinase activity at the beginning of the differentiation for up to 6 days after start of differentiation prevents differentiation whereas after day 6 there was no inhibition of differentiation anymore [43]. Thus, these results are in a good agreement with the data presented here for neural differentiation.…”

Section: Discussionsupporting

confidence: 91%

Impact of protein kinase CK2 inhibitors on proliferation and differentiation of neural stem cells

Bender

Schwind

Grundmann

et al. 2017

Heliyon

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BackgroundProtein kinases play central roles in cell and tissue development. Protein kinase CK2, an ubiquitously expressed serine/threonine kinase has severe impacts on embryo- and spermatogenesis. Since its role in neurogenesis has so far only been investigated in very few studies, we analysed the role of CK2 in neural stem cells by using two specific inhibitors.MethodsNeural stem cells were isolated from the subventricular zone of neonatal mice, using a neurosphere approach. Proliferation of the neurospheres, as well as their differentiation was investigated with and without inhibition of CK2. Changes in proliferation were assessed by counting the number and measuring the diameter of the neurospheres. Furthermore, the absolute cell numbers within the neurospheres were estimated. Differentiation was induced by retinoic acid in single cells after dissociation of the neurospheres. CK2 was inhibited at consecutive time points after induction of the differentiation process.ResultsCK2 inhibition reduced the amount and size of proliferating neurospheres dose dependently. Adding the CK2 inhibitor CX–4945 at the start of differentiation we observed a dose-dependent effect of CX-4945 on cell viability and glia cell differentiation. Adding quinalizarin, a second CK2 inhibitor, at the start of differentiation led to an elevated level of apoptosis, which was accompanied by a reduced neural differentiation. Adding the CK2 inhibitors at 72 h after the start of differentiation had no effect on stem cell differentiation. Conclusion: Inhibition of CK2 influences early gliogenesis in a time point and concentration dependent manner.General significanceThe use of a CK2 inhibitor significantly affects the neural stem cell niche.

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Section: Discussionsupporting

confidence: 91%

Impact of protein kinase CK2 inhibitors on proliferation and differentiation of neural stem cells

Bender

Schwind

Grundmann

et al. 2017

Heliyon

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“…The consensus sequence contains serine or threonine residues surrounded by acidic amino acids [17]. This kinase is involved in various cellular processes, including proliferation, differentiation, apoptosis and angiogenesis [18][19][20][21]. However, the specific function of CK2 on leukocyte-endothelial cell interaction during TNF-α-induced inflammation remains elusive.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of protein kinase CK2 suppresses tumor necrosis factor (TNF)-α-induced leukocyte–endothelial cell interaction

Ampofo

Rudzitis‐Auth

Dahmke

et al. 2015

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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Inflammatory endothelial processes are regulated by the nuclear factor-κB (NF-κB) pathway, which involves phosphorylation of p65. Because p65 is a substrate of CK2, we herein investigated, whether this pleiotropic protein kinase may be a beneficial anti-inflammatory target. For this purpose, we analyzed in human dermal microvascular endothelial cells (HDMEC) the effect of CK2 inhibition by quinalizarin and CX-4945 on cell viability, adhesion molecule expression and NF-κB pathway activation. Leukocyte binding to HDMEC was assessed in an in vitro adhesion assay. Dorsal skinfold chambers in BALB/c mice were used to study leukocyte-endothelial cell interaction and leukocyte transmigration by means of repetitive intravital fluorescence microscopy and immunohistochemistry. We found that quinalizarin and CX-4945 effectively suppressed the activity of CK2 in HDMEC without affecting their viability. This was associated with a significant down-regulation of tumor necrosis factor (TNF)-α-induced E-selectin, intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 expression due to a reduction of shuttling, phosphorylation and transcriptional activity of the NF-κB complex. In consequence, leukocyte binding to quinalizarin- and CX-4945-treated HDMEC was diminished. Finally, CX-4945 treatment significantly decreased the numbers of adherent and transmigrated leukocytes in dorsal skinfold chambers exposed to TNF-α in vivo. These findings indicate that CK2 is a key regulator of leukocyte-endothelial cell interaction in inflammation by regulating the expression of E-selectin, ICAM-1 and VCAM-1 via affecting the transcriptional activity of the NF-κB complex. Accordingly, CK2 represents a promising target for the development of novel anti-inflammatory drugs.

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“…Furthermore, it was shown by several groups that CK2 seems to be an important protein kinase for a variety of different cellular processes in developing organs and organogenesis [5,6,26,27]. In the differentiation of pre-adipocytes into adipocytes, CK2 was found to be accompanied by a decrease in the protein level and in the kinase activity [28,29]. Inhibition of CK2 kinase activity at the beginning of the differentiation process prevented differentiation, whereas, inhibition of CK2 activity at later time points had no effect on the differentiation process, which indicates that CK2 only plays an early role in differentiation of pre-adipocytes into adipocytes [28].…”

mentioning

confidence: 99%

“…In the differentiation of pre-adipocytes into adipocytes, CK2 was found to be accompanied by a decrease in the protein level and in the kinase activity [28,29]. Inhibition of CK2 kinase activity at the beginning of the differentiation process prevented differentiation, whereas, inhibition of CK2 activity at later time points had no effect on the differentiation process, which indicates that CK2 only plays an early role in differentiation of pre-adipocytes into adipocytes [28]. There is another example where CK2 contributes to differentiation processes of cells.…”

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confidence: 99%

Protein Kinase CK2 and Angiogenesis

Montenarh¹

2014

Adv Clin Exp Med

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CK2 is an ubiquitously expressed protein kinase, which is composed of two catalytic a-and a'-and two noncatalytic b-subunits. CK2 protein levels and kinase activity is elevated in rapidly proliferating cells including cancer cells. There is increasing evidence that CK2 also plays an essential role in angiogenesis, either by interaction or phosphorylation of growth factors or by phosphorylation or binding to proteins in signalling cascades, which are implicated in angiogenesis. Over the last ten years a great number of inhibitors for CK2 were detected, two of them are now in clinical phase II trials for the treatment of cancer patients. Some of these inhibitors were also found to be active in the inhibition of angiogenesis. Thus, CK2 inhibitors probably together with inhibitors of other signalling molecules involved in angiogenesis might be powerful tools for the treatment of cancer and cancer connected angiogenesis (Adv Clin Exp Med 2014, 23, 2, 153-158).

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Protein kinase CK2 is implicated in early steps of the differentiation of pre-adipocytes into adipocytes

Cited by 27 publications

References 31 publications

Impact of protein kinase CK2 inhibitors on proliferation and differentiation of neural stem cells

Impact of protein kinase CK2 inhibitors on proliferation and differentiation of neural stem cells

Inhibition of protein kinase CK2 suppresses tumor necrosis factor (TNF)-α-induced leukocyte–endothelial cell interaction

Protein Kinase CK2 and Angiogenesis

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