Protein kinase-catalyzed membrane phosphorylation and its possible relationship to the role of calcium in the adrenergic regulation of cardiac contraction

Wollenberger, A; Will, Horst

doi:10.1016/0024-3205(78)90085-1

Cited by 71 publications

(11 citation statements)

References 55 publications

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“…cause premature return of electromechanical activity. 3 Endogenously released catecholamines present in the solution may increase basal myocardial metabolism and promote calcium entry into the cell, diminishing the beneficial effects of cardioplegia.22 23 The blood-containing solution (mean hematocrit 6.3%) contained significantly more oxygen than the clear crystalloid solution (3.35 vs 0.51 vol%, p = .001). If better myocardial protection were achieved by the blood-containing solution because of its greater oxygen content, then more oxygen should be available to the heart, taken up by the heart, and utilized by the myocardium.…”

Section: Resultsmentioning

confidence: 99%

A randomized comparison of crystalloid and blood-containing cardioplegic solutions in 60 patients.

Buttner¹,

Karp²,

Rêves³

et al. 1984

Circulation

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To determine whether adding blood to a cardioplegic solution affects myocardial preservation, a randomized prospective study was carried out in 60 patients undergoing coronary revascularization to compare the effects of crystalloid potassium cardioplegics (group C) and potassium cardioplegic solutions to which blood has been added ( 973-982, 1984. ALTHOUGH blood-containing cardioplegic solutions have been shown to provide adequate myocardial protection during periods of aortic occlusion,'-' their use in preference to blood-free crystalloid solutions is still controversial. The inclusion of blood in cardioplegic solutions was devised as a means to enhance their myocardial protective characteristics, mainly through increased oxygen-carrying capacity. However, the value of blood as a means of delivery of oxygen to the myocardium has been questioned.9-'' The benefit of blood as a constituent of the cardioplegic solution may vary depending on the temperature and method of administration.3' 10 To explore whether addition of blood to a cardioplegic solution could affect myocardial preservation, a randomized prospective study was conducted to compare the effects of blood-free crystalloid and blood-containing cardioplegic solutions on markers of myocardial metabolism, myocardial damage, MethodsSixty patients scheduled for elective coronary artery revascularization were studied in a protocol approved by the institutional review board of the University of Alabama in Birmingham.They were assigned to receive either a blood-containing (group B, n = 31) or a clear crystalloid (

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Section: Resultsmentioning

confidence: 99%

A randomized comparison of crystalloid and blood-containing cardioplegic solutions in 60 patients.

Buttner¹,

Karp²,

Rêves³

et al. 1984

Circulation

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“…The catecholamine-elicited activation of adenylate cyclase (Drummond and Duncan, 1970) which catalyzes the increase in cAMP (Dobson et al, 1976) and in turn leads to activation of cAMP-dependent protein kinase (Brown et al, 1978;Dobson, 1978a) appears to be important in both the activation of glycogen phosphorylase (Dobson, 1978a) and perhaps enhanced contractile state via mechanisms involving protein phosphorylation (Wollenberger and Will, 1978;Krebs and Beavo, 1979;Dobson, 1981).…”

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confidence: 99%

Mechanism of adenosine inhibition of catecholamine-induced responses in heart.

Dobson¹

1983

Circulation Research

194

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SUMMARY. The properties of adenosine inhibition of catecholamine-induced responses were investigated, using an isolated rat heart preparation. Perfusion of hearts with 0.1 (IM isoproterenol increased myocardial cAMP content 2.8-fold, activation of cAMP-dependent protein kinase 4.4-fold, phosphorylase a formation 3.4-fold, left ventricular pressure 1.8-fold, rate of ventricular pressure development 2.1-fold, and rate of ventricular relaxation 2.2-fold within 1 minute. When perfused with the isoproterenol, 10 /IM adenosine reduced the catecholamine-produced increase in cAMP, cAMP-dependent protein kinase, and phosphorylase by 30-40%, and the elevation in left ventricular pressure and rate of ventricular pressure development by 40-70% within 40 seconds. More than 2 minutes were required for the nucleoside to significantly reduce the isoproterenol-elicited increase in the rate of ventricular relaxation. Perfusion of adenosine alone at concentrations from 0.1 to 10 fjM were without effect on the above parameters. Theophylline at 50 [IM had no effect alone on the above parameters but blocked the inhibitory actions of adenosine on the isoproterenol-induced responses. In the presence of 15 mM Mg ++ adenosine reduced by approximately 56% the 2-fold increase in myocardial membrane adenylate cyclase activity produced by 1 fiM isoproterenol without affecting basal or fluoride-stimulated activity. Adenosine also reduced the isoproterenol-induced increase in enzyme activity assayed at 1-2 mM Mg ++ , a level that more closely approximates the intracellular activity of the ion. The results suggest that physiological concentrations of adenosine attenuate the catecholamine-induced increase in cAMP content, cAMP-dependent protein kinase activation, phosphorylase a formation, and contractile parameters in the working heart, via reducing the /?-adrenergic activation of adenylate cyclase. (Circ Res 52:151-160, 1983)

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“…AMP-dependent stimulation of the Cal+-pump in sarcolemmal preparations was reported in [4,7]. The limited degree of purity of these preparations did, however, not allow an unequivocal attribution of Ca2+ -dependent activities to sarcolemmal fragments.…”

Section: Cyclicmentioning

confidence: 99%

“…Notwithstanding a number of different properties both Ca*+-ATPases are expected to be stimulated upon exposure of the mammalian heart to /3-adrenergic agonists * Permanent address: Institute of Physiology, Department of Medicine (Charite), Humboldt University Berlin, Hessische Strasse 3-4, DDR-1040 Berlin, GDR Abbreviations: C subunit, catalytic subunit of the cyclic adenosine 3',5'-monophosphate-dependent protein kinase; cyclic AMP, cyclic adenosine 3',5'-monophosphate;. Hepes, 4-(2-hydroxyethyl)-l-piperazine-ethanesulfonic acid; EGTA, ethylene glycol bis@-aminoethylether) NJ'-tetraacetic acid; pNPP, p-nitrophenylphosphate; TFP, trifluoperazine hydrochloride; SL, sarcolemma; SR, sarcoplasmic reticulum and other cyclic AMP-raising agents [4,5]. Cyclic AMP-dependent stimulation of Ca*+-transport by membranes of the SR is well documented.…”

Section: Introductionmentioning

confidence: 99%

Potentiating effect of calmodulin and catalytic subunit of cyclic AMP‐dependent protein kinase on ATP‐dependent Ca²⁺‐transport by cardiac sarcolemma

Vetter¹,

Haase²,

Will³

1982

FEBS Letters

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Highly purified sarcolemmal membranes were prepared from pig heart homogenates by differential and density gradient centrifugations. The membrane fragments exhibit ATP-dependent Ca2+-transport and Na+/Cal+-exchange activities. ATP-dependent Ca2+-transport (K@" = 0.3 PM; Vmax = 4.6 nmol Ca2+.mg protein-' . min-') is not stimulated by oxalate. Ca 2+-uptake is also not supported by p-nitrophenylphosphate. Preincubation of sarcolemma with MgATP, calmodulin and catalytic subunit of cyclic AMP-dependent protein kinase stimulates active Ca 2+-transport 1.8-fold. The effects of calmodulin and catalytic subunit are potentiating rather than additive. A large portion of the Ca2+ additionally accumulated after prephosphorylation of membranes is exchangable for Na+ via the Na+/CaT+-exchange system.

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Protein kinase-catalyzed membrane phosphorylation and its possible relationship to the role of calcium in the adrenergic regulation of cardiac contraction

Cited by 71 publications

References 55 publications

A randomized comparison of crystalloid and blood-containing cardioplegic solutions in 60 patients.

A randomized comparison of crystalloid and blood-containing cardioplegic solutions in 60 patients.

Mechanism of adenosine inhibition of catecholamine-induced responses in heart.

Potentiating effect of calmodulin and catalytic subunit of cyclic AMP‐dependent protein kinase on ATP‐dependent Ca²⁺‐transport by cardiac sarcolemma

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Protein kinase-catalyzed membrane phosphorylation and its possible relationship to the role of calcium in the adrenergic regulation of cardiac contraction

Cited by 71 publications

References 55 publications

A randomized comparison of crystalloid and blood-containing cardioplegic solutions in 60 patients.

A randomized comparison of crystalloid and blood-containing cardioplegic solutions in 60 patients.

Mechanism of adenosine inhibition of catecholamine-induced responses in heart.

Potentiating effect of calmodulin and catalytic subunit of cyclic AMP‐dependent protein kinase on ATP‐dependent Ca2+‐transport by cardiac sarcolemma

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Potentiating effect of calmodulin and catalytic subunit of cyclic AMP‐dependent protein kinase on ATP‐dependent Ca²⁺‐transport by cardiac sarcolemma