Protein Kinase C α Associates with Phospholipase D1 and Enhances Basal Phospholipase D Activity in a Protein Phosphorylation-Independent Manner in Human Melanoma Cells

Oka, Masahiro; Kageshita, Toshiro; Ono, Tomomichi; Goto, Akinobu; Kuroki, Toshio; Ichihashi, Masamitsu

doi:10.1046/j.1523-1747.2003.12300.x

Cited by 17 publications

(15 citation statements)

References 58 publications

(56 reference statements)

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“…It is germane to notice some parallels with our AL-CAM positive transcription profile (Table 1), which shows overexpression of PKCα, RhoE and GEM (a raslike GTPase), and suppression of PKCε in metastatic melanoma cells. Upregulation of PKCα may be more generally related to melanoma progression [65][66][67]. Both RhoE and GEM apparently antagonize actin stress fiber formation catalyzed by RhoA [68][69][70][71][72].…”

Section: Alcam Function: Dependence On Extracellular and Intracellulamentioning

confidence: 99%

Activated leukocyte cell adhesion molecule (ALCAM/CD166): Signaling at the divide of melanoma cell clustering and cell migration?

Swart

Lunter

Kilsdonk

et al. 2005

Cancer Metastasis Rev

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Orchestrated modulation of cell adhesion is essential for development and homeostasis in multicellular organisms. It optimizes embedding of the cell in its dynamic environment and facilitates appropriate cell responses and intercellular communication. Chronic disturbance of this delicate equilibrium causes defects in tissue architecture and sometimes cancer. In tumor cell biology, dynamic control of adhesion molecules is important to proceed through the metastatic cascade and to allow cell release from the primary tumor, invasion of the surrounding matrix, intravasation and adhesion to vascular endothelial cells to facilitate extravasation. Intertwined and multiple adhesive interactions rather than individual interactions presumably play critical roles in neoplastic development. Yet, knowledge of the contribution of each individual adhesion molecule is essential to unravel this network of interactions. This review will focus on activated leukocyte cell adhesion molecule (ALCAM/CD166) and its role in human melanoma progression. It is hypothesized that ALCAM may function as a cell surface sensor to register local growth saturation and to regulate cellular signaling and dynamic responses.

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Section: Alcam Function: Dependence On Extracellular and Intracellulamentioning

confidence: 99%

Activated leukocyte cell adhesion molecule (ALCAM/CD166): Signaling at the divide of melanoma cell clustering and cell migration?

Swart

Lunter

Kilsdonk

et al. 2005

Cancer Metastasis Rev

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“…Expression of PKC isoforms in cultured human melanocytes and melanoma cell lines [5] was examined by immunoblot analysis as described previously [6] (Fig. 1a and b).…”

Section: To the Editormentioning

confidence: 99%

“…Four melanocytic nevus lesions, 14 primary (seven cases of acral lentiginous melanoma, five cases of superficial spreading melanoma, one case of lentigo maligna melanoma, one case of mucous melanoma) melanoma lesions and nine metastatic melanoma lesions were obtained from patients who underwent surgery in the Department of Dermatology at Kumamoto University School of Medicine, Kumamoto, Japan. Immunohistochemical staining for PKC isoforms was carried out as described previously [6]. described previously [7].…”

Section: To the Editormentioning

confidence: 99%

Expression of PKC isoforms in human melanocytic cells in situ

Oka

Kageshita

Hsu

et al. 2006

Journal of Dermatological Science

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“…On the other hand, the DGK inhibitor increases DAG levels, and the accumulation of DAG increases PKC activity (Chibalin et al, 2008). PKCα is known to enhance phospholipase D (PLD) activity in human melanoma cells (Oka et al, 2003). PKC inhibitors increase melanin content and tyrosinase protein levels in B16F10 cells.…”

Section: Discussionmentioning

confidence: 99%

Diacylglycerol kinase regulates tyrosinase expression and function in human melanocytes

Kawaguchi

Namiki

Valencia

et al. 2013

Journal of Dermatological Science

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Diacylglycerol increases the melanin content of human melanocytes in vitro and increases the pigmentation of guinea pig skin in vivo, but the mechanism(s) underlying those effects remain unknown. In this study, we characterized the role of diacylglycerol kinase (DGK), which phosphorylates diacylglycerol to generate phosphatidic acid, in the regulation of pigmentation. Ten isoforms of DGK have been identified, and we show that DGKζ is the most abundant isoform expressed by human melanocytic cells. Melanin content, tyrosinase activity and tyrosinase protein levels were significantly reduced by a DGK inhibitor, but tyrosinase and MITF mRNA levels were not changed by that inhibition, and there were no effects on the expression of other melanogenesis-related proteins. Isoform-specific siRNAs showed that knockdown of DGKζ decreased melanin content and tyrosinase expression in melanocytic cells. Over-expression of DGKζ increased tyrosinase protein levels, but did not increase tyrosinase mRNA levels. Glycosidase digestion revealed that inhibition of DGK reduced only the mature form of tyrosinase and the decrease of tyrosinase resulting from DGK inhibition could be blocked partially by protease inhibitors. These results suggest that DGK regulates melanogenesis via modulation of the post-translational processing of tyrosinase, which may be related with the protein degradation machinery.

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Protein Kinase C α Associates with Phospholipase D1 and Enhances Basal Phospholipase D Activity in a Protein Phosphorylation-Independent Manner in Human Melanoma Cells

Cited by 17 publications

References 58 publications

Activated leukocyte cell adhesion molecule (ALCAM/CD166): Signaling at the divide of melanoma cell clustering and cell migration?

Activated leukocyte cell adhesion molecule (ALCAM/CD166): Signaling at the divide of melanoma cell clustering and cell migration?

Expression of PKC isoforms in human melanocytic cells in situ

Diacylglycerol kinase regulates tyrosinase expression and function in human melanocytes

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