Protein kinase C epsilon in cell division: Control of abscission

Abstract

Cell division requires the separation and partitioning of sister chromatids to opposite ends of the cell before an actomyosin ring contracts the membrane in between during cytokinesis. The final irreversible step occurs during abscission when the ring breaks down and the membrane is sealed in its place. The physical mechanics of contraction depend on RhoA, which is stimulated by a centralspindlin complex around the cell equator. However exactly how these events are reversed to allow actomyosin breakdown and abscission were not well understood. Here we will discuss new findings that implicate Protein Kinase C epsilon (PKCepsilon) as a regulator of RhoA signalling required for abscission.

The Multifunctional Protein Kinase C-ε in Cancer Development and Progression

“…Phosphorylation of PKCɛ at three sites (Ser350, Ser346 and Ser368) leads to its binding with 14-3-3 [117,118]. The PKCɛ-14-3-3 complex is required for the final abscission step in cytokinesis [119]. Rho A GTPase helps with the contraction of actomyosin ring that creates the furrow between the two poles of a dividing cell [119].…”

“…The PKCɛ-14-3-3 complex is required for the final abscission step in cytokinesis [119]. Rho A GTPase helps with the contraction of actomyosin ring that creates the furrow between the two poles of a dividing cell [119]. However, inhibition of Rho A activity is implicated in the final “pinching off” of two daughter cells.…”

“…However, inhibition of Rho A activity is implicated in the final “pinching off” of two daughter cells. It is suggested that PKCɛ-14-3-3 complex inhibits Rho A and thereby brings about the abscission step of cytokinesis [117,119]. Another study showed that PKCɛ-dependent phosphorylation of ZO-1 at Ser168 is required for the completion of cytokinesis [120].…”

PKCɛ switches Aurora B specificity to exit the abscission checkpoint

“…Protein kinase C (PKC) has been implicated in completion of the final stages of mitosis16171819. In particular, activity of the epsilon isoform (PKCɛ) has been shown to be required for successful exit from cytokinesis through a combination of signalling pathways involving RhoA (refs 17, 18) and ZO-1 (ref.…”

Αναστολή Της PKC-ε Και Μηχανισμοί Ογκογένεσης Στο ΚΝΣ

“…Πιο σημαντικά, απώλεια ή αναστολή αυτού του λειτουργικού συμπλέγματος μεταξύ των δύο πρωτεϊνών έχει ως αποτέλεσμα την καθυστέρηση της αποκοπής των θυγατρικών κυττάρων κατά την κυτοκίνηση [122,354].…”