Protein Kinase A Regulates Cholinergic Gene Expression in PC12 Cells: REST4 Silences the Silencing Activity of Neuron-Restrictive Silencer Factor/REST

Regulation of the NRSF/REST gene by methylation and CREB affects the cellular phenotype of small-cell lung cancer

“…In this investigation, the splice variant sNRSF was preferentially detected in SCLC and its appearance correlated with a reduction of the full-length transcript. The sNRSF splice variant is subject to controversial discussion (Magin et al, 2002;Gurrola-Diaz et al, 2003), as it may even activate neuronal transcripts (Shimojo et al, 1999). Possibly, binding of the splice variant that lacks the CoREST repressor domain does not serve all the functions described for the full-length NRSF/REST transcript (Palm et al, 1998).…”

Zebrafish Prickle1b mediates facial branchiomotor neuron migration via a farnesylation-dependent nuclear activity

“…We also generated a truncated version of mCherry-tagged REST (mCherryzhREST4), which is similar to the neuron-specific REST4 splice variant. In mammals, REST4 is thought to act as a natural dominant-negative by preventing full-length REST from binding to DNA (Palm et al, 1998;Shimojo et al, 1999). Injection of mRNA encoding mCherry-zhREST4 resulted in a partial disruption of FBMN migration in 47.6% of embryos (Fig.…”

Reduced expression of the neuron restrictive silencer factor permits transcription of glycine receptor α1 subunit in small-cell lung cancer cells

“…The NRSF gene encodes a protein with nine zinc-finger domains that is expressed at high levels in non-neural tissues (Chong et al, 1995;Schoenherr and Anderson, 1995;Scholl et al, 1996). It is regulated by alternative splicing generating isoforms with four, five or nine zincfinger motifs that have been extensively characterized in the rat (Palm et al, 1998(Palm et al, , 1999Shimojo et al, 1999Shimojo et al, , 2001. In humans, an isoform with four zinc-finger domains, hREST-1, has been described (Schoenherr and Anderson, 1995).…”