Protein Isoelectric Point and Helicobacter pylori

Xia, Xuhua

doi:10.1007/978-3-319-90684-3_17

Cited by 4 publications

(4 citation statements)

References 849 publications

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“…However, it should be remembered that this mechanism is not obligatory, but rather may help to facilitate adhesion. The interaction of OMVs with bacterial cells is conditioned by both the interaction of eDNA with many positively charged H. pylori proteins [ 113 ] and also directly by the presence of numerous adhesins incorporated into OMVs [ 101 , 102 ]. The processes described above take place in the early stages of biofilm formation (the microcolony formation), which seems to be confirmed by studies suggesting the importance of eDNA in the early but not late stages of H. pylori biofilm formation [ 24 ].…”

Section: Holistic Model Describing the Transition Of H mentioning

confidence: 99%

Biofilm Formation as a Complex Result of Virulence and Adaptive Responses of Helicobacter pylori

et al. 2020

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Helicobacter pylori is a bacterium that is capable of colonizing a host for many years, often for a lifetime. The survival in the gastric environment is enabled by the production of numerous virulence factors conditioning adhesion to the mucosa surface, acquisition of nutrients, and neutralization of the immune system activity. It is increasingly recognized, however, that the adaptive mechanisms of H. pylori in the stomach may also be linked to the ability of this pathogen to form biofilms. Initially, biofilms produced by H. pylori were strongly associated by scientists with water distribution systems and considered as a survival mechanism outside the host and a source of fecal-oral infections. In the course of the last 20 years, however, this trend has changed and now the most attention is focused on the biomedical aspect of this structure and its potential contribution to the therapeutic difficulties of H. pylori. Taking into account this fact, the aim of the current review is to discuss the phenomenon of H. pylori biofilm formation and present this mechanism as a resultant of the virulence and adaptive responses of H. pylori, including morphological transformation, membrane vesicles secretion, matrix production, efflux pump activity, and intermicrobial communication. These mechanisms will be considered in the context of transcriptomic and proteomic changes in H. pylori biofilms and their modulating effect on the development of this complex structure.

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Section: Holistic Model Describing the Transition Of H mentioning

confidence: 99%

Biofilm Formation as a Complex Result of Virulence and Adaptive Responses of Helicobacter pylori

et al. 2020

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“…To discuss whether these values predicted from the model fitting are reasonable, we estimated GLUT1′s charge based on its amino acid sequence. Protein charges primarily result from ionizable side chains of specific amino acids, , influenced by the surrounding environment pH. The amino acid sequences of GLUT1 from the Uniprot database and how we calculated the charge of the cytoplasmic, transmembrane, and extracellular regions of GLUT1 at different pH levels based on the p K a values of the ionizable amino acids are shown in the SI.…”

Section: Resultsmentioning

confidence: 99%

Rapid Enrichment of a Native Multipass Transmembrane Protein via Cell Membrane Electrophoresis through Buffer pH and Ionic Strength Adjustment

Liu,

Huang,

Chao

2024

J. Am. Chem. Soc.

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Supported membrane electrophoresis is a promising technique for collecting membrane proteins in native bilayer environments. However, the slow mobility of typical transmembrane proteins has impeded the technique's advancement. Here, we successfully applied cell membrane electrophoresis to rapidly enrich a 12-transmembrane helix protein, glucose transporter 1 with antibodies (GLUT1 complex), by tuning the buffer pH and ionic strength. The identified conditions allowed the separation of the GLUT1 complex and a lipid probe, Fast-DiO, within a native-like environment in a few minutes. A force model was developed to account for distinct electric and drag forces acting on the transmembrane and aqueous-exposed portion of a transmembrane protein as well as the electroosmotic force. This model not only elucidates the impact of size and charge properties of transmembrane proteins but also highlights the influence of pH and ionic strength on the driving forces and, consequently, electrophoretic mobility. Model predictions align well with experimentally measured electrophoretic mobilities of the GLUT1 complex and Fast-DiO at various pH and ionic strengths as well as with several lipid probes, lipid-anchored proteins, and reconstituted membrane proteins from previous studies. Force analyses revealed the substantial membrane drag of the GLUT1 complex, significantly slowing down electrophoretic mobility. Besides, the counterbalance of similar magnitudes of electroosmotic and electric forces results in a small net driving force and, consequently, reduced mobility under typical neutral pH conditions. Our results further highlight how the size and charge properties of transmembrane proteins influence the suitable range of operating conditions for effective movement, providing potential applications for concentrating and isolating membrane proteins within this platform.

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“…This method is not applicable in many instances because of the low penetration depth and geometrical obstacles. Other methods utilize an electric field, especially in the form of pulses (pulse electric field (PEF)) [ 124 ] and osmotic shock [ 125 , 126 ].…”

Section: Future Outlook: New Antiphagentsmentioning

confidence: 99%

Bacteriophage Challenges in Industrial Processes: A Historical Unveiling and Future Outlook

Kamiński,

Paczesny

2024

Pathogens

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Humans have used fermentation processes since the Neolithic period, mainly to produce beverages. The turning point occurred in the 1850s, when Louis Pasteur discovered that fermentation resulted from the metabolism of living microorganisms. This discovery led to the fast development of fermented food production. The importance of industrial processes based on fermentation significantly increased. Many branches of industry rely on the metabolisms of bacteria, for example, the dairy industry (cheese, milk, yogurts), pharmaceutical processes (insulin, vaccines, antibiotics), or the production of chemicals (acetone, butanol, acetic acid). These are the mass production processes involving a large financial outlay. That is why it is essential to minimize threats to production. One major threat affecting bacteria-based processes is bacteriophage infections, causing substantial economic losses. The first reported phage infections appeared in the 1930s, and companies still struggle to fight against phages. This review shows the cases of phage infections in industry and the most common methods used to prevent phage infections.

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Protein Isoelectric Point and Helicobacter pylori

Cited by 4 publications

References 849 publications

Biofilm Formation as a Complex Result of Virulence and Adaptive Responses of Helicobacter pylori

Biofilm Formation as a Complex Result of Virulence and Adaptive Responses of Helicobacter pylori

Rapid Enrichment of a Native Multipass Transmembrane Protein via Cell Membrane Electrophoresis through Buffer pH and Ionic Strength Adjustment

Bacteriophage Challenges in Industrial Processes: A Historical Unveiling and Future Outlook

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