2010
DOI: 10.1007/s00216-010-3708-6
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Protein immobilization at gold–thiol surfaces and potential for biosensing

Abstract: Self-assembled monolayers (SAMs) provide a convenient, flexible and simple system to tailor the interfacial properties of metals, metal oxides and semiconductors. Monomolecular films prepared by self-assembly are attractive for several exciting applications because of the unique possibility of making the selection of different types of terminal functional groups and as emerging tools for nanoscale observation of biological interactions. The tenability of SAMs as platforms for preparing biosurfaces is reviewed … Show more

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Cited by 137 publications
(111 citation statements)
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“…To study the DET reaction of cyt c, 11-MUA was used because cyt c is electrostatically immobilized on a SAM of carboxylic acid-terminated alkanethiols via lysine residues. 13 A pair of redox peaks for cyt c were observed for all of the electrodes. The surface coverage (! )…”
Section: Direct Electron Transfer Of Immobilized Cyt Cmentioning
confidence: 93%
See 1 more Smart Citation
“…To study the DET reaction of cyt c, 11-MUA was used because cyt c is electrostatically immobilized on a SAM of carboxylic acid-terminated alkanethiols via lysine residues. 13 A pair of redox peaks for cyt c were observed for all of the electrodes. The surface coverage (! )…”
Section: Direct Electron Transfer Of Immobilized Cyt Cmentioning
confidence: 93%
“…SAM-modified electrodes can be used to immobilize proteins with the appropriate orientation on the electrode surface. 13 In our previous studies, the surface coverage of redox-active cytochrome c (cyt c) on AuNPmodified electrodes was 167 times higher than the roughly estimated monolayer coverage of 15 pmol cm ¹2 . The current density for the oxidation of D-fructose using D-fructose dehydrogenase (FDH) immobilized on AuNP-modified electrodes reached 14.3 mA cm ¹2 in the presence of 200 mM D-fructose.…”
Section: Introductionmentioning
confidence: 94%
“…SAM layers are a versatile coating as there is a wide variety of terminating functional groups which can be used to interact with a large number of molecules. This allows the SAM to 31 or a smart surface having the initial surface properties of the formed film which changes in response to an external stimulus, e.g., chemical, optical, electric, or thermal. 32 …”
Section: Fabrication Of the Transducersmentioning
confidence: 99%
“…Using alkanethiol molecules, highly organized and stable platforms with reproducibility and easy preparation can be developed [2][3][4]. Moreover, alkanethiol molecules containing terminal functional groups, such as carboxylic acid moieties, are suit-able to covalently immobilize proteins [5][6][7][8]. However, this method requires the incorporation of an activation step, usually performed with N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), to attach the protein to the SAM [8][9][10].…”
Section: Introductionmentioning
confidence: 99%