Protein identification in mass-spectrometry imaging

Mascini, Nadine E.; Heeren, Ron M. A.

doi:10.1016/j.trac.2012.06.008

Cited by 25 publications

(32 citation statements)

References 62 publications

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“…Proteins can be detected and imaged directly from biological tissue typically up to a molecular mass of about 30 kDa [141][142][143] . This method has been widely applied in the past, but is lacking specificity and on-tissue-identification capability.…”

Section: Intact-protein Imagingmentioning

confidence: 99%

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Mass Spectrometry Imaging of Biomolecular Information

2014

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Section: Intact-protein Imagingmentioning

confidence: 99%

“…Applications of on-tissue protein digestion followed by MALDI MSI are manifold 142 . Human skin peptides were imaged by MALDI MSI after on-tissue digestion to differentiate the individual skin layers 150 .…”

Section: On-tissue Enzymatic Digestion For Peptide and Protein Imagingmentioning

confidence: 99%

Mass Spectrometry Imaging of Biomolecular Information

2014

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“…However, identification of species within an MS image can be labor intensive in some cases. Targeted in situ MALDI MS/MS analysis for the identification of lipids and small molecules is generally routine using current technology, but identification of proteins and peptides pose greater challenges [25]. …”

Section: Maldi Ims As a Robust Bioanalytical Toolmentioning

confidence: 99%

MALDI imaging mass spectrometry: Spatial molecular analysis to enable a new age of discovery

Gessel

Norris

Caprioli

2014

Journal of Proteomics

241

204

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Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) combines the sensitivity and selectivity of mass spectrometry with spatial analysis to provide a new dimension for histological analyses to provide unbiased visualization of the arrangement of biomolecules in tissue. As such, MALDI IMS has the capability to become a powerful new molecular technology for the biological and clinical sciences. In this review, we briefly describe several applications of MALDI IMS covering a range of molecular weights, from drugs to proteins. Current limitations and challenges are discussed along with recent developments to address these issues.

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“…The combination of MALDI with IM-MS enhances the reliability of identification in imaging applications [34] and allows utilization of DIA fragmentation with this ionization method.…”

Section: Ion-mobility Ms (Im-ms)mentioning

confidence: 99%