Protein Functionalized Nanodiamond Arrays

Abstract

Various nanoscale elements are currently being explored for bio-applications, such as in bio-images, bio-detection, and bio-sensors. Among them, nanodiamonds possess remarkable features such as low bio-cytotoxicity, good optical property in fluorescent and Raman spectra, and good photostability for bio-applications. In this work, we devise techniques to position functionalized nanodiamonds on self-assembled monolayer (SAMs) arrays adsorbed on silicon and ITO substrates surface using electron beam lithography techniques. The nanodiamond arrays were functionalized with lysozyme to target a certain biomolecule or protein specifically. The optical properties of the nanodiamond-protein complex arrays were characterized by a high throughput confocal microscope. The synthesized nanodiamond-lysozyme complex arrays were found to still retain their functionality in interacting with E. coli.

Adsorption of influenza A and B viruses on detonation nanodiamonds materials

“…After reviewing publications on biological properties of DND, we conclude that DND actively interacts with nano-sized drugs and substances of protein nature such as enzymes and bacteria [2,9]. We have shown that DND, soot and their complexes with PANI are also able to interact with influenza viruses and the interaction doesn't vary for different antigenic structure of the surface proteins-hemagglutinins of influenza B or influenza A (H1,H3,H5) viruses.…”

Diamant‐Quantensensoren in der Biologie

“…Zusätzlich konnten verschiedene funktionelle Gruppen auf die Oberfläche von NDs gebunden werden, die wie bereits beschrieben eine kovalente Anbindung von Biomolekülen ermöglichen (Abbildung ). Eine derartige Oberflächenmodifikation ist derzeitig die einzige Methode, um NDs mit biologischen Funktionalitäten auszustatten, und sie wurde bereits angewendet, um Antikörper, DNA, Enzyme oder weitere funktionale Proteine anzubinden. Allerdings neigen die rohen, aus der direkten Synthese stammenden NDs leicht dazu, Aggregate zu bilden, was zum Teil auf ihr hohes Oberfläche‐zu‐Volumen‐Verhältnis zurückzuführen ist.…”

High-Frequency Electron Paramagnetic Resonance Spectroscopy of Nitroxide-Functionalized Nanodiamonds in Aqueous Solution

“…In many of those applications, functional biological molecules need to be tailored to the ND surfaces. Even though many approaches to attach biological molecules to ND surfaces have been investigated [5,8,12,[14][15][16], it remains challenging to monitor behaviors of the grafted molecules because the presence of ND hampers the employment of standard techniques (e.g., chromatography and optical spectroscopy).…”