Protein-Functionalized Hairy Diamond Nanoparticles

Abstract

Diazonium salt chemistry and atom transfer radical polymerization (ATRP) were combined in view of preparing new bioactive hairy diamond nanoparticles containing, or potentially containing, nitrogen-vacancy (NV) fluorescent centers (fluorescent nanodiamonds, or fNDs). fNDs were modified by ATRP initiators using the electroless reduction of the diazonium salt BF(4)(-),(+)N(2)-C(6)H(4)-CH(CH(3))-Br. The strongly bound aryl groups -C(6)H(4)-CH(CH(3))-Br efficiently initiated the ATRP of tert-butyl methacrylate (tBMA) at the surface of the nanodiamonds, which resulted in obtaining ND-PtBMA hybrids. The grafted chain thickness, estimated from X-ray photoelectron spectroscopy (XPS), was found to increase linearly with respect to time before reaching a plateau value of ca. 2 nm. These nanoobjects were further hydrolyzed into ND-PMAA (where PMAA is the poly(methacrylic acid) graft) and further decorated by bovine serum albumin through the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling procedure.

Electrografting of calix[4]arenediazonium salts to form versatile robust platforms for spatially controlled surface functionalization

“…This result agrees well with the height of calix [4] non-electroactive calixarene 2. On gold samples, the average calix [4]arene coverage α on the gold surface could be expressed from F 1s and Au 4f peak intensities related to calix [4]arenes layers and the gold substrate, respectively 49 . The calculation (details provided in the Supplementary Methods) leads to a surface coverage α of the gold substrate by the calix [4]arene 2 molecules of 79%.…”

Inhibition of type 1 fimbriae-mediated Escherichia coli adhesion and biofilm formation by trimeric cluster thiomannosides conjugated to diamond nanoparticles

“…[40][41][42]72 ND particles are completely inert, optically transparent, biocompatible and moreover, easily functionalizable via a variety of strategies depending on their intended application. [43][44][45][46][47][48][49][50] Although their in vivo toxicity depends in particular on their surface characteristics (as well as the nature of the ligands they carry on their surface), 51 ND particles have thus far been reported not to induce significant cytotoxicity in a variety of cell types. [51][52][53][54] The demonstration that our 1 st -generation sugar-conjugated NDs do show marked anti-adhesive activity in cell-based assays without displaying toxicity against eukaryotic cells conforted us in our choice of particle and convinced us that sugar-NDs should indeed be further pursued as biomaterials.…”

Antimicrobial activity of menthol modified nanodiamond particles

“…One of the advantages of NDs over other carbon-based materials such as fullerenes and carbon nanotubes is that they are completely inert, optically transparent, biocompatible and can be functionalized in many ways depending on their intended ultimate application [24], [25], [26], [27], [28], [29], [30] and [31]. Although their in vivo toxicity depends on their particular surface characteristics [32], ND particles do not induce significant cytotoxicity in a variety of cell types [32], [33], [34] and [35] and have been used in a variety of biomedical applications.…”