Protein film formation on cell culture surfaces investigated by quartz crystal microbalance with dissipation monitoring and atomic force microscopy

Wargenau, Andreas; Fekete, Natalie; Béland, Ariane V.; Sabbatier, Gad; Bowden, O.; Boulanger, Mariève D.; Hoesli, Corinne A.

doi:10.1016/j.colsurfb.2019.110447

Cited by 8 publications

(3 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Monocytes cultured both in FEP bags or TCPS plates differentiated into professional DCs as judged by their comparable capacity to generate functionally-competent antigen-specific cytotoxic CD8 + T cells. Our functional findings are consistent with our observations when studying protein adsorption on different model surfaces: the abundance of albumin in our medium led to the formation of a secondary protein layer with very similar mechanical properties and topography on polystyrene and fluoropolymer surfaces [ 34 ]. These studies were conducted in serum-free media and protein adlayer properties may differ between TCPS and FEP with media that contain serum (e.g.…”

Section: Discussionsupporting

confidence: 90%

Closing the system: production of viral antigen-presenting dendritic cells eliciting specific CD8+ T cell activation in fluorinated ethylene propylene cell culture bags

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background A major obstacle to anti-viral and -tumor cell vaccination and T cell immunotherapy is the ability to produce dendritic cells (DCs) in a suitable clinical setting. It is imperative to develop closed cell culture systems to accelerate the translation of promising DC-based cell therapy products to the clinic. The objective of this study was to investigate whether viral antigen-loaded monocyte-derived DCs (Mo-DCs) capable of eliciting specific T cell activation can be manufactured in fluorinated ethylene propylene (FEP) bags. Methods Mo-DCs were generated through a protocol applying cytokine cocktails combined with lipopolysaccharide or with a CMV viral peptide antigen in conventional tissue culture polystyrene (TCPS) or FEP culture vessels. Research-scale (< 10 mL) FEP bags were implemented to increase R&D throughput. DC surface marker profiles, cytokine production, and ability to activate antigen-specific cytotoxic T cells were characterized. Results Monocyte differentiation into Mo-DCs led to the loss of CD14 expression with concomitant upregulation of CD80, CD83 and CD86. Significantly increased levels of IL-10 and IL-12 were observed after maturation on day 9. Antigen-pulsed Mo-DCs activated antigen-responsive CD8+ cytotoxic T cells. No significant differences in surface marker expression or tetramer-specific T cell activating potency of Mo-DCs were observed between TCPS and FEP culture vessels. Conclusions Our findings demonstrate that viral antigen-loaded Mo-DCs produced in downscaled FEP bags can elicit specific T cell responses. In view of the dire clinical need for closed system DC manufacturing, FEP bags represent an attractive option to accelerate the translation of promising emerging DC-based immunotherapies.

show abstract

Section: Discussionsupporting

confidence: 90%

Closing the system: production of viral antigen-presenting dendritic cells eliciting specific CD8+ T cell activation in fluorinated ethylene propylene cell culture bags

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Studies have shown that cells do not adhere directly to the surface but to proteins, in turn adsorbed on the first surface layer [52]. The protein layers formed within the first few minutes of contact with the culture medium consist almost exclusively of albumin [53]. This initial protein adsorption behavior that affect cell-adhesive protein such as fibronectin could subsequently regulate the cell adhesion via focal contacts [54,55].…”

Section: Protein Adsorptionmentioning

confidence: 99%

Ultrafast laser processing of nanostructured patterns for the control of cell adhesion and migration on titanium alloy

Khalil

Sedao

Papa

et al. 2021

2021 Conference on Lasers and Electro-Optics Europe &Amp; European Quantum Electronics Conference (CLEO/Europe-EQEC)

View full text Add to dashboard Cite

Femtosecond laser texturing is a promising surface functionalization technology to improve the integration and durability of dental and orthopedic implants. Four different surface topographies were obtained on titanium-6aluminum-4vanadium plates by varying laser processing parameters and strategies: surfaces presenting nanostructures such as laser-induced periodic surface structures (LIPSS) and 'spikes', associated or not with more complex multiscale geometries combining micro-pits, nanostructures and stretches of polished areas. After sterilization by heat treatment, LIPSS and spikes were characterized to be highly hydrophobic, whereas the original polished surfaces remained hydrophilic. Human mesenchymal stem cells (hMSCs) grown on simple nanostructured surfaces were found to spread less with an increased motility (velocity, acceleration, tortuosity), while on the complex surfaces, hMSCs decreased their migration when approaching the micro-pits and preferentially positioned their nucleus inside them. Moreover, focal adhesions of hMSCs were notably located on polished zones rather than on neighboring nanostructured areas where the protein adsorption was lower. All these observations indicated that hMSCs were spatially controlled and mechanically strained by the laser-induced topographies. The nanoscale structures influence surface wettability and protein adsorption and thus influence focal adhesions formation and finally induce shape-based mechanical constraints on cells, known to promote osteogenic differentiation.

show abstract

“…93 Another technique, quartz crystal microbalance (QCM), which can detect deposition on a surface fairly sensitively, is also underexploited in this regard. As QCM coupled with AFM has been available for some time, 94 we are likely to see its application in corona formation too. Similarly, small-angle Xray scattering (SAXS) analysis, which allows looking at kinetic changes during protein aggregation, may also be found useful.…”

Section: Molecular Pharmaceuticsmentioning

confidence: 99%

How Corona Formation Impacts Nanomaterials as Drug Carriers

Gupta

Roy

2020

Mol. Pharmaceutics

View full text Add to dashboard Cite

As drugs/drug carriers, upon encountering physiological fluids, nanoparticles adsorb biological molecules almost immediately to form a biocorona, which is often simply called a corona. Once the corona is formed, it dictates the subsequent fate of the drug nanoparticle as a therapeutic agent. Protein adsorption on micron-size or even bigger particles was originally described by the Vroman effect. It has served as a useful framework to understand the corona formation. Proteins that are irreversibly adsorbed on nanoparticles form what is called a hard corona. Beyond that is the exchangeable population of proteins, which constitute the dynamic structure called a soft corona. More than the abundance, the affinity of the proteins toward the nanoparticles decides which ones end up in the corona. For example, the more common serum albumin, which is deposited initially, is displaced by fibrinogen, which has a higher affinity for gold nanoparticles. The curvature of the particle is a crucial parameter with bigger particles generally able to bind a more diverse population of proteins from the physiological milieu. The earlier perception of the corona formation being a challenge for drug targeting, etc. has been turned into an opportunity by engineering corona to manipulate properties like circulating half-lives, capacity to evade the immune system, and targeting or even overcoming the blood−brain barrier. The most commonly used techniques for particle characterization, including dynamic light scattering (DLS), differential sedimentation centrifugation, transmission electron microscopy (TEM), and SDS-PAGE, have been adopted to study corona formation in the past. Many newer tools, for example, a combination of capillary electrophoresis with mass spectrometry, are being used to study the corona composition. The comparison of interlaboratory results is a problem because of the lack of standard protocols. This has hindered the ability to obtain more precise information about the corona composition. That, in turn, affects our prospects to use nanoparticles as drugs/drug carriers. This overview is an attempt to assess our understanding of corona formation critically and to outline the complexities involved in gaining precise information. The discussion is largely focused on findings of the last year or so.

show abstract

Protein film formation on cell culture surfaces investigated by quartz crystal microbalance with dissipation monitoring and atomic force microscopy

Cited by 8 publications

References 47 publications

Closing the system: production of viral antigen-presenting dendritic cells eliciting specific CD8+ T cell activation in fluorinated ethylene propylene cell culture bags

Closing the system: production of viral antigen-presenting dendritic cells eliciting specific CD8+ T cell activation in fluorinated ethylene propylene cell culture bags

Ultrafast laser processing of nanostructured patterns for the control of cell adhesion and migration on titanium alloy

How Corona Formation Impacts Nanomaterials as Drug Carriers

Contact Info

Product

Resources

About