Protein expression profiling in interstitial cystitis/painful bladder syndrome: A pilot study of proteins associated with inflammation, apoptosis, and angiogenesis

Shie, Jia-Heng; Liu, Hsin-Tzu; Kuo, Hann‐Chorng

doi:10.1016/j.urols.2012.10.003

Cited by 3 publications

(4 citation statements)

References 36 publications

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“…18,19 In preliminary studies, we found that the angiogenesis marker interleukin-8 and VEGF were suppressed under onabotulinumtoxinA therapy. 20 According to our preliminary results (protein array and western blotting), we found that the degree of glomerulations and angiogenic factors could be reduced because of the inflammatory suppression by repeated onabotulinumtoxinA injections in some patients with IC/BPS. 17 However, in this study, the glomerulaions after repeated onabotulinumtoxinA injections did not significantly change, although there was a change in VEGF.…”

Section: Commentmentioning

confidence: 77%

Down Regulation of Vascular Endothelial Growth Factor Is Associated With Decreased Inflammation After Intravesical OnabotulinumtoxinA Injections Combined With Hydrodistention for Patients With Interstitial Cystitis—Clinical Results and Immunohistochemistry Analysis

Peng

Jhang

Shie

et al. 2013

Urology

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Section: Commentmentioning

confidence: 77%

Down Regulation of Vascular Endothelial Growth Factor Is Associated With Decreased Inflammation After Intravesical OnabotulinumtoxinA Injections Combined With Hydrodistention for Patients With Interstitial Cystitis—Clinical Results and Immunohistochemistry Analysis

Peng

Jhang

Shie

et al. 2013

Urology

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“…The findings revealed the involvement of several signal transduction pathways in the pathophysiology of IC/BPS 24 . The elevated protein expressions in IC/BPS bladders showed a decrease after intravesical injection of botulinum toxin A, including VEGF, platelet factor 4, IL-1β, IL-8, CXCL16, and TIMP-4; these findings reflect the changes in bladder condition after effective treatment 24 . However, in this study, none of the functional proteins or cytokine levels (BDNF, TGFβ1, IL-2, I-6, IL-8, IL-1β, TNFα) showed a decrease after PRP treatment, with the exception of urinary NGF.…”

Section: Discussionmentioning

confidence: 91%

“…In our previous proteomic study, we investigated the markers of inflammation, angiogenesis, and urothelial cell apoptosis in IC/BPS bladder and urine. The findings revealed the involvement of several signal transduction pathways in the pathophysiology of IC/BPS 24 . The elevated protein expressions in IC/BPS bladders showed a decrease after intravesical injection of botulinum toxin A, including VEGF, platelet factor 4, IL-1β, IL-8, CXCL16, and TIMP-4; these findings reflect the changes in bladder condition after effective treatment 24 .…”

Section: Discussionmentioning

confidence: 91%

“…Urine samples were collected at baseline and at each study time-point for quantification of markers using a multiplexed immunoassay. A panel of 13 proteins including inflammatory markers, growth factors, cytokines/ chemokines, and neurotrophic factors were chosen based on our previous proteomics study on the protein expression profiling in IC/BPS 24 . Markers of inflammation, angiogeneis, growth factors, and cytokines were measured using a multiplex, magnetic bead-based immunoassay (MILLIPLEX® MAP; EMD Millipore, Billerica, MA, USA) according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

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Repeated intravesical injections of platelet-rich plasma improve symptoms and alter urinary functional proteins in patients with refractory interstitial cystitis

Jiang

Kuo

Jhang

et al. 2020

Sci Rep

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Repeated intravesical injections of autologous platelet-rich plasma (PRP) have been shown to improve symptoms in patients with interstitial cystitis/bladder pain syndrome (IC/BPS); however, there is a paucity of objective evidence of the effectiveness of this therapy. In this study, we investigated the changes in urinary markers after PRP treatment. Forty patients with IC/BPS who were refractory to conventional therapy received four injections of PRP at monthly intervals; 10 mL PRP solution with 2.5 times the peripheral blood platelet concentration was used. Urine levels of thirteen functional proteins, growth factors, and cytokines were assessed at baseline and at the 4th PRP injection. The clinical parameters included visual analog scale (VAS) pain score, daily urinary frequency, nocturia episodes, functional bladder capacity, and global response assessment (GRA). The GRA and symptom score significantly decreased post-treatment. In patients with GRA ≥ 2, the success rates at 1 month and at 3 months after the 4th PRP injection were 70.6% and 76.7%, respectively. The VAS pain score, frequency, and nocturia showed a significant decrease (all p < 0.05). Urinary levels of nerve growth factor, matrix metalloproteinase-13, and vascular endothelial growth factor significantly decreased post-treatment (p = 0.043, p = 0.02, and p = 0.000, respectively); platelet-derived growth factor-AB showed a significant increase (p = 0.004) at the 4th PRP treatment compared with baseline. In this study, repeated intravesical PRP injections provided significant symptom improvement in IC/BPS patients with concomitant changes in the related biomarker levels. Trial registration: ClinicalTrial.gov: NCT03104361; IRB: TCGH 105-48-A.

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Detrusor Myocyte Autophagy Protects the Bladder Function via Inhibiting the Inflammation in Cyclophosphamide-Induced Cystitis in Rats

et al. 2015

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Autophagy, a highly conserved homeostatic cellular process that removes and recycles damaged proteins and organelles in response to cellular stress, is believed to play a crucial role in the immune response and inflammation. The role of autophagy in bladder cystitis, however, has not well been clarified. Here we investigate the role of detrusor myocytes autophagy (DMA) in cyclophosphamide-induced cystitis animal model. 164 female Sprague-Dawley rats were randomized into three experimental groups and compared to three control groups, respectively. The expressions of microtubule-associated protein 1 light chain 3 (LC3), p-p70s6k (the phosphorylated form of ribosomal protein S6), SOD2 (superoxide dismutase 2) in the bladder muscular layer were measured using western blot. The co-location of LC3, alpha-smooth muscle actin (α-SMA), and autophagic vacuoles were investigated with double-labeled immunofluorescence and transmission electron microscopy (TEM). The expression of lL-1β, IL-6, IL-8, malondialdehyde (MDA), and glutathione (GSH) in the detrusor layer were analyzed using ELISA. The bladder inflammation and the number of mast cells in the muscular layer were analyzed by histology. The bladder function was evaluated using cystometry. In cyclophosphamide-induced cystitis, autophagy was detected in detrusor myocytes by increased LC3, p-p70s6k expression, and autophagosomes. However, the presence of enhanced inflammation and oxidative stress in the cyclophosphamide-treated group suggest autophagy of detrusor myocytes may not be sufficiently activated. Inflammation and oxidative stress were significantly decreased and the bladder histology and micturition function were significantly improved with rapamycin (RAPA, autophagy agonist) pre-treatment. In contrast, inflammation and oxidative stress were dramatically increased and the bladder histology and function were negatively affected with chloroquine (CQ, autophagy blocker) pre-treated. These findings preferentially provide evidence of the association between DMA and cyclophosphamide-induced cystitis in rats. The autophagy agonist RAPA significantly decreased the inflammation and protected the bladder function, which might be considered as a potential treatment for interstitial cystitis.

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Protein expression profiling in interstitial cystitis/painful bladder syndrome: A pilot study of proteins associated with inflammation, apoptosis, and angiogenesis

Cited by 3 publications

References 36 publications

Down Regulation of Vascular Endothelial Growth Factor Is Associated With Decreased Inflammation After Intravesical OnabotulinumtoxinA Injections Combined With Hydrodistention for Patients With Interstitial Cystitis—Clinical Results and Immunohistochemistry Analysis

Down Regulation of Vascular Endothelial Growth Factor Is Associated With Decreased Inflammation After Intravesical OnabotulinumtoxinA Injections Combined With Hydrodistention for Patients With Interstitial Cystitis—Clinical Results and Immunohistochemistry Analysis

Repeated intravesical injections of platelet-rich plasma improve symptoms and alter urinary functional proteins in patients with refractory interstitial cystitis

Detrusor Myocyte Autophagy Protects the Bladder Function via Inhibiting the Inflammation in Cyclophosphamide-Induced Cystitis in Rats

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