Protein-enriched pea flour extract protects stored milled rice against the rice weevil, Sitophilus oryzae

Abstract

Studies were conducted to evaluate the effect of a protein-enriched pea (Pisum sativum var. Bonneville) flour extract against the rice weevil, Sitophilus oryzae in its repellency, toxicity, effect on fecundity, stability and sensory properties. Milled rice admixed with pea flour extract at 1% concentration significantly repelled S. oryzae. Mortality of S. oryzae was found to increase and fecundity was markedly suppressed, in rice treated with 1% pea flour extract. The toxicity and reproductive effects of the pea protein-enriched rice were found to be stable for a period of 5 months. The sensory characteristics of stored rice when eaten were not affected by the treatment with pea flour extract. This study indicates that the protein-enriched flour extract obtained from the Bonneville pea may be feasible to protect stored milled rice from insect attack.

Bioactivity of Cereal- and Legume-Based Macaroni Pasta Volatiles to Adult Sitophilus granarius (L.)

“…Therefore, consistently with results of behavioural bioassays, susceptibility tests demonstrated the unsuitability of legume-based pastas as a food source for adult granary weevils. This is in fairly good agreement with previous studies reporting a significant reduction of survival and reproduction of conspecific S. oryzae adults fed with an admixture of yellow split-peas and wheat [ 30 , 31 ] or rice treated with 1% pea flour extract [ 51 ]. Similar results were obtained by Fields et al (2001) [ 25 ] with nine stored-grain beetles, including S. oryzae , S. granarius , and S. zeamais , reared on wheat kernels or flour treated with P. sativum fractions.…”

Cloning, expression, and purification of insect (Sitophilus oryzae) alpha-amylase, able to digest granular starch, in Yarrowia lipolytica host

“…Surprisingly, the highest decomposition degree was observed for the pea starch, followed by amaranth, waxy rice, and waxy corn (0.44 ± 0.004, 0.12 ± 0.004, 0.11 ± 0.01, and 0.01 ± 0.0001 μmol of reducing sugar equivalents, respectively; ±SD), with no detectable degradation of granular starch originating from potato, tapioca (both starches characterized by a high degree of amylose polymerization), wheat, or amylose-containing corn. It has been reported that pea flour is a strong repellent of S. oryzae , which makes it a promising protecting agent of stored rice and wheat grains (Pretheep-Kumar et al 2004 ). However, it has been observed that pure pea starch is not the repelling agent, but rather pea proteins, contained in the pea flour, exert the repelling effect.…”

Hexane extracts from Tephrosia vogelii Hook. f. protect stored maize against the weevil Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae)

“…Tephrosia vogelii is reportedly as repellent to insects as the neem plant (Boeke et al 2004). This might have contributed to fewer females being attracted to grains treated with the hexane extract, thus resulting in the observed drastic reduction in the number of eggs deposited, as suggested for the rice weevil S. oryzae exposed to protein-enriched pea flour-treated rice (Fields et al 2001;Pretheep-Kumar et al 2004). The two other extracts obtained using the more polar solvents, acetone and ethanol, were ineffective.…”