Protein engineering of a new recombinant peptide to increase the surface contact angle of stainless steel

Yang

Uche

et al. 2018

IJMS

Modification of metal surfaces with antimicrobial peptides is a promising approach to reduce bacterial adhesion. Here, cyclic peptides or cycloids, possessing remarkable stability and antimicrobial activities, were extracted and purified from Viola philippica Cav., and identified using mass spectrometry. Cyclotides were subsequently utilized to modify stainless steel surfaces via polydopamine-mediated coupling. The resulting cyclotide-modified surfaces were characterized by Fourier transform infrared (FTIR) spectroscopy and contact angle analysis. The antibacterial capacity of these cyclotides against Staphylococcus aureus was assessed by Alamar blue assay. The antibiofilm capacity of the modified surfaces was assessed by crystal violet assay, and scanning electron microscopy (SEM). A composite of Kalata b1, Varv A, Viba 15 and Viba 17 (P1); Varv E (P2); and Viphi G (P3) were isolated and identified. FTIR analysis of the modified surfaces demonstrated that cyclotides bound to the surfaces and induced reduction of contact angles. Antimicrobial effects showed an order P3 > P1 and P2, with P3-treated surfaces demonstrating the strongest antibiofilm capacity. SEM confirmed reduced biofilm formation for P3-treated surfaces. This study provides novel evidence for cyclotides as a new class for development of antibacterial and antibiofilm agents.

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Coupling Plant-Derived Cyclotides to Metal Surfaces: An Antibacterial and Antibiofilm Study

Yang

Uche

et al. 2018

IJMS

“…Davis et al yielded a new bioorganic metal by the reaction of 12 amino acid peptide and stainless steel and the surface properties of the new material altered compared with the original metal . An antibacterial peptide obtained from gene‐engineered has been used to react with 304 stainless steel for generating antibacterial materials . An antimicrobial peptide, magainin II (MAG II), was discovered and isolated from the granular gland of the skin of the African frog, Xenopus laevis .…”

Section: Introductionmentioning

confidence: 99%

“…22 An antibacterial peptide obtained from geneengineered has been used to react with 304 stainless steel for generating antibacterial materials. 23 An antimicrobial peptide, magainin II (MAG II), was discovered and isolated from the granular gland of the skin of the African frog, Xenopus laevis. 24 MAG II has a broad spectrum of antimicrobial activity against Gram-positive and Gram-negative bacteria.…”

Section: Introductionmentioning

confidence: 99%

A biofilm resistance surface yielded by grafting of antimicrobial peptides on stainless steel surface

Surface & Interface Analysis

Yuan

Xiao

et al. 2018

Anti‐biofilm formation on the surface is a severe issue in medical implants, hull surface, and food industry. Antimicrobial peptide, magainin II, was covalently bound to stainless steel surfaces through multi‐step modification. The untreated and modified samples were analyzed by SEM‐EDS, XPS, and contact angle, respectively, which indicated the peptide was immobilized on the surfaces. The antimicrobial tests of modified samples were conducted using Staphylococcus aureus and Escherichia coli, and the results revealed that peptide modified surface decreased the biofilm and bacteria quantity of stainless steel surface.

“…Many experiments indicated that peptides with disulphide bond were easy to react with stainless steel. Ren et al designed and produced a peptide from Bacillus subtilis which could prepare a new material with good hydrophobicity by reacting with stainless steel . Some short‐chain peptides obtained by biological chemical synthesis were also applied to the synthesis of new materials …”

Section: Introductionmentioning

confidence: 99%

Peptide‐modified stainless steel with resistance capacity of Staphylococcus aureus biofilm formation

Surface & Interface Analysis

Xiao

et al. 2018

Attachment of marine fouling organisms causes biofouling. The adhesion of destructive organisms can be reduced through surface modification with antibiofilm chemicals. In this study, a direct surface modification between peptide solution with different concentrations and stainless steel was performed, and the reaction mechanism was explained by simulation of the modification process. Results of surface water contact angle and surface hardness indicated the optimal modification concentration of peptide solution was 10 μg/mL. Under the optimal concentration, peptide-modified stainless steel was prepared through the reaction between peptide and 304 stainless steel. Results of scanning electron microscopy equipped with energy dispersive spectrometry, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy demonstrated that the peptide was successfully bound on stainless steel surface. Antimicrobial activity of samples surface was tested against Staphylococcus aureus. The results illustrated that the peptide treated sample surface possesses significant antimicrobial property. The findings presented valuable information on marine antifouling researches. KEYWORDSantibiofilm, bio-modified metallic material, optimal concentration, peptide, simulationIn aquatic environment, conditioning layer induced by microorganisms is easy to form rapidly on the solid surface. 1 Bacteria then adhere on the conditioning layer and secrete biofilms. The biofilms contain extracellular protein and sticky polysaccharide, which make biofilms easy to attach on almost all surfaces exposed to the non-sterile environment and difficult to remove according to the previous research studies. 2 A large number of fouling organisms will be attracted on the biofilms, resulting in biofouling on the surface of the metal. [3][4][5] Biofouling causes great loss in many fields, including medical implants, food industry, and ship transportation. 6,7 Many methods have been utilized to reduce marine biofouling.For example, coatings with heavy metal ions have been used to reduce the adhesion of biofouling organisms. However, the spreading of the ions into the sea water will cause marine environment pollution. Green techniques should be applied into the research on the antifouling in ship transportation. Previous results showed that the changes of surface energy were related with antifouling of metal surface. Biofouling organisms will be difficult to adhere or easy to detach on the materials surface to achieve antifouling if the surface energy is low or ultralow. [8][9][10][11][12][13] A new bioorganic material with lower surface energy which yielded by an unknown reaction of peptides and metals has been reported. 14,15 It revealed that a chemical reaction of the peptide coatings with metals occurred and changed the electronic state of the metal surface. [16][17][18] Wong et al obtained a material by the reaction between polypeptide and stainless steel; moreover, the extent of the reaction could be improved via dopamine addition. 19,20 Davis e...